Imperial College London
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DrFrancescaCeroni

Faculty of Engineering, Department of Chemical Engineering

Senior Lecturer
 
 
 
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Contact

 

f.ceroni

 
 
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Location

 

510AACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Di:2021:10.1016/j.tibtech.2020.12.007,
author = {Di, Blasi R and Zouein, A and Ellis, T and Ceroni, F},
doi = {10.1016/j.tibtech.2020.12.007},
journal = {Trends in Biotechnology},
pages = {1004--1018},
title = {Genetic toolkits to design and build mammalian synthetic systems},
url = {http://dx.doi.org/10.1016/j.tibtech.2020.12.007},
volume = {39},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Construction of DNA-encoded programs is central to synthetic biology and the chosen method oftendetermines the time required to design and build constructs for testing. Here we describe and summarisekey features of the available toolkits for DNA construction for mammalian cells. We compare the differentcloning strategies based on their complexity and the time needed to generate constructs of different sizes,and we reflect on why Golden Gate toolkits now dominate due to their modular design. We look forward tofuture advances, including accessory packs to cloning toolkits that can facilitating editing, orthogonality,advanced regulation, and integration into synthetic chromosome construction.
AU  - Di,Blasi R
AU  - Zouein,A
AU  - Ellis,T
AU  - Ceroni,F
DO  - 10.1016/j.tibtech.2020.12.007
EP  - 1018
PY  - 2021///
SN  - 0167-7799
SP  - 1004
TI  - Genetic toolkits to design and build mammalian synthetic systems
T2  - Trends in Biotechnology
UR  - http://dx.doi.org/10.1016/j.tibtech.2020.12.007
UR  - https://www.sciencedirect.com/science/article/pii/S0167779920303322?via%3Dihub
UR  - http://hdl.handle.net/10044/1/85052
VL  - 39
ER  -
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