Imperial College London
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DrFrancescaCeroni

Faculty of Engineering, Department of Chemical Engineering

Senior Lecturer
 
 
 
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Contact

 

f.ceroni

 
 
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Location

 

510AACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Haynes:2012:10.1021/sb3000035,
author = {Haynes, KA and Ceroni, F and Flicker, D and Younger, A and Silver, PA},
doi = {10.1021/sb3000035},
journal = {ACS Synthetic Biology},
pages = {99--106},
title = {A sensitive switch for visualizing natural gene silencing in single cells},
url = {http://dx.doi.org/10.1021/sb3000035},
volume = {1},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - RNA interference is a natural gene expression silencing system that appears throughout the tree of life. As the list of cellular processes linked to RNAi grows, so does the demand for tools to accurately measure RNAi dynamics in living cells. We engineered a synthetic RNAi sensor that converts this negative regulatory signal into a positive output in living mammalian cells, thereby allowing increased sensitivity and activation. Furthermore, the circuit’s modular design allows potentially any microRNA of interest to be detected. We demonstrated that the circuit responds to an artificial microRNA and becomes activated when the RNAi target is replaced by a natural microRNA target (miR-34) in U2OS osteosarcoma cells. Our studies extend the application of rationally designed synthetic switches to RNAi, providing a sensitive way to visualize the dynamics of RNAi activity rather than just the presence of miRNA molecules.
AU  - Haynes,KA
AU  - Ceroni,F
AU  - Flicker,D
AU  - Younger,A
AU  - Silver,PA
DO  - 10.1021/sb3000035
EP  - 106
PY  - 2012///
SN  - 2161-5063
SP  - 99
TI  - A sensitive switch for visualizing natural gene silencing in single cells
T2  - ACS Synthetic Biology
UR  - http://dx.doi.org/10.1021/sb3000035
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305490200004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/61085
VL  - 1
ER  -
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