Imperial College London
Alternate

Professor Francis Drobniewski

Faculty of Medicine, Department of Infectious Disease

Chair in Global Health and Tuberculosis
 
 
 
//

Contact

 

f.drobniewski

 
 
//

Location

 

Commonwealth BuildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Larrouy-Maumus:2021:10.3389/fchem.2021.715890,
author = {Larrouy-Maumus, G and Broda, A and Drobniewski, F and Khor, MJ and kostrzewa},
doi = {10.3389/fchem.2021.715890},
journal = {Frontiers in Chemistry},
pages = {1--7},
title = {An improved method for rapid detection of Mycobacterium abscessus complex based on species-specific lipids fingerprint by routine MALDI-TOF},
url = {http://dx.doi.org/10.3389/fchem.2021.715890},
volume = {9},
year = {2021}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Rapid diagnostics of bacterial infection is the key to successful recovery and eradication of the disease. Currently, identification of bacteria is based on the detection of highly abundant proteins, mainly ribosomal proteins, by routine MALDI-TOF mass spectrometry. However, relying solely on proteins is limited in subspecies typing for some pathogens. This is the case for, for example, the mycobacteria belonging to the Mycobacterium abscessus (MABS) complex, which is classified into three subspecies, namely, M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense. Being able to detect bacteria accurately and rapidly at the subspecies level could not only reliably identify the pathogen causing the disease but also enable better antibiotic stewardship. For instance, M. abscessus subsp. abscessus and M. abscessus subsp. bolletii possess a functional erm41 (erythromycin ribosomal methylation gene 41) gene, whilst M. abscessus subsp. massiliense does not, resulting in differences in macrolide antibiotic (e.g., clarithromycin and azithromycin) susceptibilities. This presents a challenge for physicians when designing an appropriate treatment regimen. To address this challenge, in addition to proteins, species-specific lipids have now been considered as a game changer in clinical microbiology diagnostics. However, their extraction can be time-consuming, and analysis requires the use of apolar toxic organic solvents (e.g., chloroform). Here, we present a new method to accurately detect species and subspecies, allowing the discrimination of the mycobacteria within the MABS complex and relying on the use of ethanol. We found that a combination of the matrix named super-DHB with 25% ethanol with a bacterial suspension at McFarland 20 gave robust and reproducible data, allowing the discrimination of the bacteria within the MABS complex strains tested in this study (n = 9). Further investigations have to be conducted to validate the metho
AU  - Larrouy-Maumus,G
AU  - Broda,A
AU  - Drobniewski,F
AU  - Khor,MJ
AU  - kostrzewa
DO  - 10.3389/fchem.2021.715890
EP  - 7
PY  - 2021///
SN  - 2296-2646
SP  - 1
TI  - An improved method for rapid detection of Mycobacterium abscessus complex based on species-specific lipids fingerprint by routine MALDI-TOF
T2  - Frontiers in Chemistry
UR  - http://dx.doi.org/10.3389/fchem.2021.715890
UR  - https://www.frontiersin.org/articles/10.3389/fchem.2021.715890/full
UR  - http://hdl.handle.net/10044/1/90695
VL  - 9
ER  -
Download