Imperial College London
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Dr Chun Fui Lai

Faculty of Medicine, Department of Surgery & Cancer

Research Associate
 
 
 
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Contact

 

f.lai

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kramer:2015:nar/gkv948,
author = {Kramer, H and Lai, C and Dattani, H and Periyasamy, M and Lin, M and Ali, S and Buluwela, L},
doi = {nar/gkv948},
journal = {Nucleic Acids Research},
pages = {582--594},
title = {LRH-1 drives colon cancer cell growth by repressing the expression of the CDKN1A gene in a p53-dependent manner},
url = {http://dx.doi.org/10.1093/nar/gkv948},
volume = {44},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Liver receptor homologue 1 (LRH-1) is an orphan nuclearreceptor that has been implicated in the progressionof breast, pancreatic and colorectal cancer(CRC). To determine mechanisms underlying growthpromotion by LRH-1 in CRC, we undertook global expressionprofiling following siRNA-mediated LRH-1knockdown in HCT116 cells, which require LRH-1 forgrowth and in HT29 cells, in which LRH-1 does notregulate growth. Interestingly, expression of the cellcycle inhibitor p21 (CDKN1A) was regulated by LRH-1 in HCT116 cells. p21 regulation was not observedin HT29 cells, where p53 is mutated. p53 dependencefor the regulation of p21 by LRH-1 was confirmed byp53 knockdown with siRNA, while LRH-1-regulationof p21 was not evident in HCT116 cells where p53 hadbeen deleted. We demonstrate that LRH-1-mediatedp21 regulation in HCT116 cells does not involve alteredp53 protein or phosphorylation, and we showthat LRH-1 inhibits p53 recruitment to the p21 promoter,likely through a mechanism involving chromatinremodelling. Our study suggests an importantrole for LRH-1 in the growth of CRC cells that retainwild-type p53.
AU  - Kramer,H
AU  - Lai,C
AU  - Dattani,H
AU  - Periyasamy,M
AU  - Lin,M
AU  - Ali,S
AU  - Buluwela,L
DO  - nar/gkv948
EP  - 594
PY  - 2015///
SN  - 1362-4962
SP  - 582
TI  - LRH-1 drives colon cancer cell growth by repressing the expression of the CDKN1A gene in a p53-dependent manner
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gkv948
UR  - http://hdl.handle.net/10044/1/26516
VL  - 44
ER  -
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