Publications
35 results found
Keshavarz M, Wales DJ, Seichepine F, et al., 2023, Induced neural stem cell differentiation on a drawn fiber scaffold-toward peripheral nerve regeneration (vol 15, 055011, 2020), BIOMEDICAL MATERIALS, Vol: 18, ISSN: 1748-6041
Power M, Barbot A, Seichepine F, et al., 2023, Bistable, Pneumatically Actuated Microgripper Fabricated Using Two-Photon Polymerization and Oxygen Plasma Etching, ADVANCED INTELLIGENT SYSTEMS, Vol: 5
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- Citations: 2
Zhang D, Ren Y, Barbot A, et al., 2022, Fabrication and optical manipulation of micro-robots for biomedical applications, MATTER, Vol: 5, Pages: 3135-3160, ISSN: 2590-2393
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- Citations: 2
Petrou L, Latvanen E, Seichepine F, et al., 2022, Lateral Flow Test (LFT) detects cell-free microRNAs predictive of preterm birth directly from human plasma, Advanced NanoBiomed Research, Vol: 2, ISSN: 2699-9307
Despite extensive research toward the development of point-of-care nucleic acid tests (POC NATs) for the detection of microRNAs (miRs) from liquid biopsies, major hurdles remain including the strict requirement for extensive off-chip sample preprocessing. Herein, a nucleic acid lateral flow test (NALFT) is reported on that enables the direct detection of endogenous miRs from as little as 3 μL of plasma without the requirement for any enzyme-catalyzed target amplification or complex miR extraction steps. This is achieved through integration of a denaturing hydrogel composite material onto the LFT, allowing for near-instantaneous on-chip release of miRs from their carriers (extracellular vesicles or transport proteins) prior to detection. This next-generation LFT is sensitive enough to detect endogenous concentrations of miR-150-5p, a predictive biomarker for preterm birth (PTB) found deregulated in maternal blood from as early as 12th week of pregnancy. Herein, a key step is represented toward a first bedside test for risk-stratification during pregnancy by predicting true outcome at a very early stage. More generally, the universal and versatile nature of this novel sample preprocessing platform can further improve the robustness of existing NALFTs and facilitate their application at the POC.
Sessi V, Ibarlucea B, Seichepine F, et al., 2022, Multisite Dopamine Sensing With Femtomolar Resolution Using a CMOS Enabled Aptasensor Chip, FRONTIERS IN NEUROSCIENCE, Vol: 16
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- Citations: 5
Zhang D, Barbot A, Seichepine F, et al., 2022, Micro-object pose estimation with sim-to-real transfer learning using small dataset, Communications Physics, Vol: 5, ISSN: 2399-3650
Kassanos P, Seichepine F, Yang G-Z, 2021, A comparison of front-end amplifiers for tetrapolar bioimpedance measurements, IEEE Transactions on Instrumentation and Measurement, Vol: 70, Pages: 1-14, ISSN: 0018-9456
Many commercial benchtop impedance analyzers are incapable of acquiring accurate tetrapolar measurements, when large electrode contact impedances are present, as in bioimpedance measurements using electrodes with micrometer-sized features. External front-end amplifiers can help overcome this issue and provide high common-mode rejection ratio (CMRR) and input impedance. Several discrete component-based topologies are proposed in the literature. In this article, these are compared with new alternatives with regard to their performance in measuring known loads in the presence of electrode contact impedance models, to emulate tetrapolar bioimpedance measurements. These models are derived from bipolar impedance measurements taken from the electrodes of a tetrapolar bioimpedance sensor. Comparison with other electrode models used in the literature established that this is a good and challenging model for bioimpedance front-end amplifier evaluation. Among the examined amplifiers, one of the best performances is achieved with one of the proposed topologies based on a custom front-end with no external resistors (AD8066/AD8130). Under the specific testing conditions, it achieved an uncalibrated worst-case absolute measurement deviation of 4.4% magnitude and 4° at 20 Hz, and 2.2% and 7° at 1 MHz accordingly with loads between 10 Ω and 10 kg. Finally, the practical use of the front-end with the impedance analyzer is demonstrated in the characterization of the bioimpedance sensor, in saline solutions of varying conductivities (2.5-20 mS/cm) to obtain its cell constant. This article serves as a guide for evaluating and choosing front-end amplifiers for tetrapolar bioimpedance measurements both with and without impedance analyzers for practical/clinical applications and material/sensor characterization.
Lee H-T, Seichepine F, Yang G-Z, 2020, Microtentacle Actuators Based on Shape Memory Alloy Smart Soft Composite, ADVANCED FUNCTIONAL MATERIALS, Vol: 30, ISSN: 1616-301X
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- Citations: 20
Keshavarz M, Wales DJ, Seichepine F, et al., 2020, Induced neural stem cell differentiation on a drawn fiber scaffold-toward peripheral nerve regeneration, Biomedical Materials, Vol: 15, ISSN: 1748-6041
To achieve regeneration of long sections of damaged nerves, restoration methods such as direct suturing or autologous grafting can be inefficient. Solutions involving biohybrid implants, where neural stem cells are grown in vitro on an active support before implantation, have attracted attention. Using such an approach, combined with recent advancements in microfabrication technology, the chemical and physical environment of cells can be tailored in order to control their behaviors. Herein, a neural stem cell polycarbonate fiber scaffold, fabricated by 3D printing and thermal drawing, is presented. The combined effect of surface microstructure and chemical functionalization using poly-ʟ-ornithine (PLO) and double-walled carbon nanotubes (DWCNTs) on the biocompatibility of the scaffold, induced differentiation of the neural stem cells (NSCs) and channeling of the neural cells was investigated. Upon treatment of the fiber scaffold with a suspension of DWCNTs in PLO (0.039 gL-1) and without recombinants a high degree of differentiation of NSCs into neuronal cells was confirmed by using nestin, galactocerebroside (GalC) and doublecortin (Dcx) immunoassays. These findings illuminate the potential use of this biohybrid approach for the realization of future nerve regenerative implants.
Kassanos P, Berthelot M, Kim JA, et al., 2020, Smart sensing for surgery from tethered devices to wearables and implantables, IEEE Systems Man and Cybernetics Magazine, Vol: 6, Pages: 39-48, ISSN: 2333-942X
Recent developments in wearable electronics have fueled research into new materials, sensors, and microelectronic technologies for the realization of devices that have increased functionality and performance. This is further enhanced by advances in fabr ication methods and printing techniques, stimulating research on implantables and the advancement of existing medical devices. This article provides an overview of new designs, embodiments, fabrication methods, instrumentation, and informatics as well as the challenges in developing and deploying such devices and clinical applications that can benefit from them. The need for and use of these technologies across the perioperative surgical-care pathway are highlighted, along with a vision for the future and how these tools can be adopted by potential end users and health-care systems.
Kassanos P, Seichepine F, Kassanos I, et al., 2020, Development and Characterization of a PCB-Based Microfluidic YChannel*, 2020 42nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) in conjunction with the 43rd Annual Conference of the Canadian Medical and Biological Engineering Society, Publisher: IEEE
Barbot A, Power M, Seichepine F, et al., 2020, Liquid seal for compact micro-piston actuation at capillary tip, Science Advances, Vol: 6, ISSN: 2375-2548
Actuators at the tip of a sub-millimetric catheter could facilitatein vivointer-ventional procedures at cellular scales by enabling tissue biopsy, manipulationor supporting active micro-optics. However the dominance of frictional forcesat this scale makes classical mechanism problematic. In this paper, we reportthe design of a micro-scale piston, with a maximum dimension of 150μm,fabricated with two-photon lithography onto the tip of 140μm diameter cap-illaries. An oil drop method is used to create a seal between the piston andthe cylinder which prevents any leakage below 185 mbar pressure differencewhile providing lubricated friction between moving parts. This piston gener-ates forces that increase linearly with pressure up to 130μN without breakingthe liquid seal. The practical value of the design is demonstrated with its inte-gration with a micro-gripper that can grasp, move and release 50μm micro-spheres. Such a mechanism opens the way to micron-size catheter actuation.
Barbot A, Power M, Seichepine F, et al., 2020, Liquid seal for compact micropiston actuation at the capillary tip., Sci Adv, Vol: 6
Actuators at the tip of a submillimetric catheter could facilitate in vivo interventional procedures at cellular scales by enabling tissue biopsy and manipulation or supporting active micro-optics. However, the dominance of frictional forces at this scale makes classical mechanism problematic. Here, we report the design of a microscale piston, with a maximum dimension of 150 μm, fabricated with two-photon lithography onto the tip of 140-μm-diameter capillaries. An oil drop method is used to create a seal between the piston and the cylinder that prevents any leakage below 185-mbar pressure difference while providing lubricated friction between moving parts. This piston generates forces that increase linearly with pressure up to 130 μN without breaking the liquid seal. The practical value of the design is demonstrated with its integration with a microgripper that can grasp, move, and release 50-μm microspheres. Such a mechanism opens the way to micrometer-size catheter actuation.
Barbot A, Tan H, Power M, et al., 2019, Floating magnetic microrobots for fiber functionalization, SCIENCE ROBOTICS, Vol: 4, ISSN: 2470-9476
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- Citations: 38
Dudina A, Seichepine F, Chen Y, et al., 2019, Monolithic CMOS sensor platform featuring an array of 9 ' 216 carbon-nanotube-sensor elements and low-noise, wide-bandwidth and wide-dynamic-range readout circuitry, SENSORS AND ACTUATORS B-CHEMICAL, Vol: 279, Pages: 255-266, ISSN: 0925-4005
Kassanos P, Seichepine F, Keshavarz M, et al., 2019, Towards a Flexible Wrist-Worn Thermotherapy and Thermoregulation Device, 19th Annual IEEE International Conference on Bioinformatics and Bioengineering (BIBE), Publisher: IEEE, Pages: 644-648, ISSN: 2471-7819
Li B, Tan H, Anastasova-Ivanova S, et al., 2019, A bioinspired 3D micro-structure for graphene-based bacteria sensing, Biosensors and Bioelectronics, Vol: 123, Pages: 77-84, ISSN: 0956-5663
Nature is a great source of inspiration for the development of solutions for biomedical problems. We present a novel biosensor design utilizing two-photon polymerisation and graphene to fabricate an enhanced biosensing platform for the detection of motile bacteria. A cage comprising venous valve-inspired directional micro-structure is fabricated around graphene-based sensing electronics. The asymmetric 3D micro-structure promotes motile cells to swim from outside the cage towards the inner-most chamber, resulting in concentrated bacteria surrounding the central sensing region, thus enhancing the sensing signal. The concentrating effect is proved across a range of cell cultures - from 101 CFU/ml to 109 CFU/ml. Fluorescence analysis shows a 3.38–3.5 times enhanced signal. pH sensor presents a 2.14–3.08 times enhancement via the detection of cellar metabolite. Electrical measurements demonstrate an 8.8–26.7 times enhanced current. The proposed platform provides a new way of leveraging bio-inspired 3D printing and 2D materials for the development of sensing devices for biomedical applications.
Kassanos P, Seichepine F, Yang G-Z, 2019, Characterization and Modeling of a Flexible Tetrapolar Bioimpedance Sensor and Measurements of Intestinal Tissues, 19th Annual IEEE International Conference on Bioinformatics and Bioengineering (BIBE), Publisher: IEEE, Pages: 686-690, ISSN: 2471-7819
Kassanos P, Seichepine F, Wales D, et al., 2019, Towards a Flexible/Stretchable Multiparametric Sensing Device for Surgical and Wearable Applications, IEEE Biomedical Circuits and Systems Conference (BioCAS), Publisher: IEEE, ISSN: 2163-4025
Seichepine F, Rothe J, Dudina A, et al., 2017, Dielectrophoresis-assisted integration of 1024 carbon nanotube sensors into a CMOS microsystem, Advanced Materials, Vol: 29, ISSN: 1521-4095
Carbon-nanotube (CNT)-based sensors offer the potential to detect single-molecule events and picomolar analyte concentrations. An important step toward applications of such nanosensors is their integration in large arrays. The availability of large arrays would enable multiplexed and parallel sensing, and the simultaneously obtained sensor signals would facilitate statistical analysis. A reliable method to fabricate an array of 1024 CNT-based sensors on a fully processed complementary-metal-oxide-semiconductor microsystem is presented. A high-yield process for the deposition of CNTs from a suspension by means of liquid-coupled floating-electrode dielectrophoresis (DEP), which yielded 80% of the sensor devices featuring between one and five CNTs, is developed. The mechanism of floating-electrode DEP on full arrays and individual devices to understand its self-limiting behavior is studied. The resistance distributions across the array of CNT devices with respect to different DEP parameters are characterized. The CNT devices are then operated as liquid-gated CNT field-effect-transistors (LG-CNTFET) in liquid environment. Current dependency to the gate voltage of up to two orders of magnitude is recorded. Finally, the sensors are validated by studying the pH dependency of the LG-CNTFET conductance and it is demonstrated that 73% of the CNT sensors of a given microsystem show a resistance decrease upon increasing the pH value.
Sessi V, Seichepine F, Pregl S, et al., 2016, Integrating bottom-up grown silicon nanowires on a CMOS chip to realize high-density transistor arrays for chemical sensing, Pages: 1118-1119
We report on a new sensor platform for chemical sensing featuring 1024 individually addressable field-effect transistors (FETs), based on small bundles of nominally undoped Si nanowires (SiNWs). The SiNW devices are integrated directly on top of control and readout circuits fabricated in complementary metal-oxide-semiconductor (CMOS) technology. The sensing mechanism is based on electrical field tuning of the conductivity through Schottky junctions at the metal/Si nanowire interfaces [1]. We show integration, electrical measurements and pH-sensing of a high-density array (≥100 devices/mm2) with a sensing area of 1 × 5μm2 per sensor element.
Dudina A, Seichepine F, Chen Y, et al., 2016, Switch-matrix-based Monolithic CMOS Platform Featuring a Large Array of Carbon Nanotube Sensor Elements and a 96-channel Readout Circuitry, 30th Eurosensors Conference, Publisher: ELSEVIER SCIENCE BV, Pages: 916-919, ISSN: 1877-7058
Collet M, Salomon S, Klein NY, et al., 2015, Large-Scale Assembly of Single Nanowires through Capillary-Assisted Dielectrophoresis, ADVANCED MATERIALS, Vol: 27, Pages: 1268-1273, ISSN: 0935-9648
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- Citations: 69
Collet M, Salomon S, Klein NY, et al., 2015, Nanowires: Large‐Scale Assembly of Single Nanowires through Capillary‐Assisted Dielectrophoresis (Adv. Mater. 7/2015), Advanced Materials, Vol: 27, Pages: 1304-1304, ISSN: 0935-9648
Seichepine F, Rothe J, Dudina A, et al., 2014, CMOS-integrated high-density arrays of carbon nanotube sensors, Pages: 1835-1837
We report on the integration of single and small bundles of carbon nanotube (CNT) biosensors on a CMOS-integrated array featuring 1024 devices. The system has been designed to allow for direct and versatile readout of each nanosensor. A parallel integration of the CNT nanosensors has been achieved by using semi-sacrificial electrodes and dielectrophoresis (DEP). The developed sensing platform will be used for high-resolution chemical monitoring of complex biological systems. Here, we present the on-chip CNT integration process and statistical analysis of the fabricated devices.
Beduer A, Seichepine F, Flahaut E, et al., 2012, Elucidation of the Role of Carbon Nanotube Patterns on the Development of Cultured Neuronal Cells, LANGMUIR, Vol: 28, Pages: 17363-17371, ISSN: 0743-7463
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- Citations: 37
Salomon S, Leichle T, Dezest D, et al., 2012, Arrays of nanoelectromechanical biosensors functionalized by microcontact printing, NANOTECHNOLOGY, Vol: 23, ISSN: 0957-4484
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- Citations: 16
Loubinoux I, Vaysse L, Béduer A, et al., 2012, Cellules souches et récupération motrice après AVC, Annals of Physical and Rehabilitation Medicine, Vol: 55, Pages: e150-e151, ISSN: 1877-0657
Loubinoux I, Vaysse L, Béduer A, et al., 2012, Non-invasive brain stimulations and post-stroke motor recovery, Annals of Physical and Rehabilitation Medicine, Vol: 55, Pages: e151-e152, ISSN: 1877-0657
Beduer A, Seichepine F, Flahaut E, et al., 2012, A simple and versatile micro contact printing method for generating carbon nanotubes patterns on various substrates, MICROELECTRONIC ENGINEERING, Vol: 97, Pages: 301-305, ISSN: 0167-9317
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- Citations: 8
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