Imperial College London
Alternate

ProfessorGeoffBaldwin

Faculty of Natural Sciences, Department of Life Sciences

Professor of Synthetic and Molecular Biology
 
 
 
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Contact

 

+44 (0)20 7594 5288g.baldwin

 
 
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Location

 

508Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Storch:2020:synbio/ysaa010,
author = {Storch, M and Haines, MC and Baldwin, GS},
doi = {synbio/ysaa010},
journal = {Synthetic Biology},
pages = {ysaa010--ysaa010},
title = {DNA-BOT: a low-cost, automated DNA assembly platform for synthetic biology},
url = {http://dx.doi.org/10.1093/synbio/ysaa010},
volume = {5},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Multi-part DNA assembly is the physical starting point for many projects in Synthetic and Molecular Biology. The ability to explore a genetic design space by building extensive libraries of DNA constructs is essential for creating programmed biological systems. With multiple DNA assembly methods and standards adopted in the Synthetic Biology community, automation of the DNA assembly process is now receiving serious attention. Automation will enable larger builds using less researcher time, while increasing the accessible design space. However, these benefits currently incur high costs for both equipment and consumables. Here, we address this limitation by introducing low-cost DNA assembly with BASIC on OpenTrons (DNA-BOT). For this purpose, we developed an open-source software package and demonstrated the performance of DNA-BOT by simultaneously assembling 88 constructs composed of 10 genetic parts, evaluating the promoter, ribosome binding site and gene order design space for a three-gene operon. All 88 constructs were assembled with high accuracy, at a consumables cost of $1.50-$5.50 per construct. This illustrates the efficiency, accuracy and affordability of DNA-BOT, making it accessible for most labs and democratizing automated DNA assembly.
AU  - Storch,M
AU  - Haines,MC
AU  - Baldwin,GS
DO  - synbio/ysaa010
EP  - 010
PY  - 2020///
SN  - 2397-7000
SP  - 010
TI  - DNA-BOT: a low-cost, automated DNA assembly platform for synthetic biology
T2  - Synthetic Biology
UR  - http://dx.doi.org/10.1093/synbio/ysaa010
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32995552
UR  - http://hdl.handle.net/10044/1/84417
VL  - 5
ER  -
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