Imperial College London
Alternate

ProfessorGeoffBaldwin

Faculty of Natural Sciences, Department of Life Sciences

Professor of Synthetic and Molecular Biology
 
 
 
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Contact

 

+44 (0)20 7594 5288g.baldwin

 
 
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Location

 

508Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Beal:2021:10.1371/journal.pone.0252263,
author = {Beal, J and Baldwin, GS and Farny, NG and Gershater, M and Haddock-Angelli, T and Buckley-Taylor, R and Dwijayanti, A and Kiga, D and Lizarazo, M and Marken, J and de, Mora K and Rettberg, R and Sanchania, V and Selvarajah, V and Sison, A and Storch, M and Workman, CT},
doi = {10.1371/journal.pone.0252263},
journal = {PLoS One},
title = {Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs},
url = {http://dx.doi.org/10.1371/journal.pone.0252263},
volume = {16},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Reproducibility is a key challenge of synthetic biology, but the foundation of reproducibility is only as solid as the reference materials it is built upon. Here we focus on the reproducibility of fluorescence measurements from bacteria transformed with engineered genetic constructs. This comparative analysis comprises three large interlaboratory studies using flow cytometry and plate readers, identical genetic constructs, and compatible unit calibration protocols. Across all three studies, we find similarly high precision in the calibrants used for plate readers. We also find that fluorescence measurements agree closely across the flow cytometry results and two years of plate reader results, with an average standard deviation of 1.52-fold, while the third year of plate reader results are consistently shifted by more than an order of magnitude, with an average shift of 28.9-fold. Analyzing possible sources of error indicates this shift is due to incorrect preparation of the fluorescein calibrant. These findings suggest that measuring fluorescence from engineered constructs is highly reproducible, but also that there is a critical need for access to quality controlled fluorescent calibrants for plate readers.
AU  - Beal,J
AU  - Baldwin,GS
AU  - Farny,NG
AU  - Gershater,M
AU  - Haddock-Angelli,T
AU  - Buckley-Taylor,R
AU  - Dwijayanti,A
AU  - Kiga,D
AU  - Lizarazo,M
AU  - Marken,J
AU  - de,Mora K
AU  - Rettberg,R
AU  - Sanchania,V
AU  - Selvarajah,V
AU  - Sison,A
AU  - Storch,M
AU  - Workman,CT
DO  - 10.1371/journal.pone.0252263
PY  - 2021///
SN  - 1932-6203
TI  - Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0252263
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000664640700006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252263
UR  - http://hdl.handle.net/10044/1/94119
VL  - 16
ER  -
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