Imperial College London
Alternate

ProfessorGeoffBaldwin

Faculty of Natural Sciences, Department of Life Sciences

Professor of Synthetic and Molecular Biology
 
 
 
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Contact

 

+44 (0)20 7594 5288g.baldwin

 
 
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Location

 

508Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dwijayanti:2022:nar/gkab1301,
author = {Dwijayanti, A and Storch, M and Stan, G-B and Baldwin, GS},
doi = {nar/gkab1301},
journal = {Nucleic Acids Research},
title = {A modular RNA interference system for multiplexed gene regulation},
url = {http://dx.doi.org/10.1093/nar/gkab1301},
volume = {50},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The rational design and realisation of simple-to-use genetic control elements that are modular, orthogonal and robust is essential to the construction of predictable and reliable biological systems of increasing complexity. To this effect, we introduce modular Artificial RNA interference (mARi), a rational, modular and extensible design framework that enables robust, portable and multiplexed post-transcriptional regulation of gene expression in Escherichia coli. The regulatory function of mARi was characterised in a range of relevant genetic contexts, demonstrating its independence from other genetic control elements and the gene of interest, and providing new insight into the design rules of RNA based regulation in E. coli, while a range of cellular contexts also demonstrated it to be independent of growth-phase and strain type. Importantly, the extensibility and orthogonality of mARi enables the simultaneous post-transcriptional regulation of multi-gene systems as both single-gene cassettes and poly-cistronic operons. To facilitate adoption, mARi was designed to be directly integrated into the modular BASIC DNA assembly framework. We anticipate that mARi-based genetic control within an extensible DNA assembly framework will facilitate metabolic engineering, layered genetic control, and advanced genetic circuit applications.
AU  - Dwijayanti,A
AU  - Storch,M
AU  - Stan,G-B
AU  - Baldwin,GS
DO  - nar/gkab1301
PY  - 2022///
SN  - 0305-1048
TI  - A modular RNA interference system for multiplexed gene regulation
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gkab1301
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35061908
UR  - https://academic.oup.com/nar/article/50/3/1783/6513569
UR  - http://hdl.handle.net/10044/1/93984
VL  - 50
ER  -
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