Publications
76 results found
Shah AV, Birdsey GM, Reynolds LE, et al., 2014, The endothelial transcription factor ERG promotes vascular stability and growth through Wnt/β-catenin signaling, ANGIOGENESIS, Vol: 17, Pages: 715-715, ISSN: 0969-6970
Dufton N, Almagro LO, Birdsey G, et al., 2014, THE ENDOTHELIAL TRANSCRIPTION FACTOR ERG INHIBITS VASCULAR INFLAMMATION, HEART, Vol: 100, Pages: A115-A116, ISSN: 1355-6037
Thornton C, Alrashed F, Calay D, et al., 2014, METHOTREXATE: A NOVEL MECHANISM FOR VASCULOPROTECTION IN CHRONIC SYSTEMIC INFLAMMATION, 15th Annual European Congress of Rheumatology (EULAR), Publisher: BMJ PUBLISHING GROUP, Pages: 363-364, ISSN: 0003-4967
Thornton C, Al-Rashed F, Calay D, et al., 2014, METHOTREXATE: A NOVEL MECHANISM FOR VASCULOPROTECTION IN CHRONIC SYSTEMIC INFLAMMATION, Annual Meeting of the British-Society-for-Rheumatology and British-Health-Professionals-in-Rheumatology, Publisher: OXFORD UNIV PRESS, Pages: 35-35, ISSN: 1462-0324
Amsellem V, Dryden NH, Martinelli R, et al., 2014, ICAM-2 regulates vascular permeability and N-cadherin localization through ezrin-radixin-moesin (ERM) proteins and Rac-1 signalling, CELL COMMUNICATION AND SIGNALING, Vol: 12, ISSN: 1478-811X
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- Citations: 18
Shapiro SE, Nowak AA, Wooding C, et al., 2014, The von Willebrand factor predicted unpaired cysteines are essential for secretion, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 12, Pages: 246-254, ISSN: 1538-7933
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- Citations: 14
Birdsey GM, Shah A, Reynolds L, et al., 2014, Regulation of vascular development and angiogenesis by the ETS transcription factor Erg through canonical Wnt signalling, ANGIOGENESIS, Vol: 17, Pages: 276-277, ISSN: 0969-6970
Kostourou V, Lechertier T, Reynolds LE, et al., 2013, FAK-heterozygous mice display enhanced tumour angiogenesis, Nature Communications, Vol: 4, ISSN: 2041-1723
Genetic ablation of endothelial focal adhesion kinase (FAK) can inhibit pathological angiogenesis, suggesting that loss of endothelial FAK is sufficient to reduce neovascularization. Here we show that reduced stromal FAK expression in FAK-heterozygous mice unexpectedly enhances both B16F0 and CMT19T tumour growth and angiogenesis. We further demonstrate that cell proliferation and microvessel sprouting, but not migration, are increased in serum-stimulated FAK-heterozygous endothelial cells. FAK-heterozygous endothelial cells display an imbalance in FAK phosphorylation at pY397 and pY861 without changes in Pyk2 or Erk1/2 activity. By contrast, serum-stimulated phosphorylation of Akt is enhanced in FAK-heterozygous endothelial cells and these cells are more sensitive to Akt inhibition. Additionally, low doses of a pharmacological FAK inhibitor, although too low to affect FAK autophosphorylation in vitro, can enhance angiogenesis ex vivo and tumour growth in vivo. Our results highlight a potential novel role for FAK as a nonlinear, dose-dependent regulator of angiogenesis where heterozygous levels of FAK enhance angiogenesis.
Bauer A, Thornton CC, Mylroie H, et al., 2012, Investigation of the regulatory role of heme oxygenase-1 and its products in VEGF-mediated angiogenesis, 6th European Meeting on Vascular Biology and Medicine (EMVBM), Publisher: ELSEVIER SCIENCE INC, Pages: 345-345, ISSN: 1537-1891
Birdsey GM, Dryden NH, Shah AV, et al., 2012, The transcription factor Erg regulates expression of HDAC6 and multiple pathways involved in endothelial cell migration and angiogenesis, 6th European Meeting on Vascular Biology and Medicine (EMVBM), Publisher: ELSEVIER SCIENCE INC, Pages: 348-349, ISSN: 1537-1891
Dryden NH, Sperone A, Martin-Almedina S, et al., 2012, The transcription factor erg controls endothelial cell quiescence by repressing activity of Nuclear Factor (NF)-kappa B p65, Journal of Biological Chemistry, Vol: 287, Pages: 12331-12342, ISSN: 0021-9258
The interaction of transcription factors with specific DNA sequences is critical for activation of gene expression programs. In endothelial cells (EC), the transcription factor NF-κB is important in the switch from quiescence to activation, and is tightly controlled to avoid excessive inflammation and organ damage. Here we describe a novel mechanism that controls the activation of NF-κB in EC. The transcription factor Erg, the most highly expressed ETS member in resting EC, controls quiescence by repressing proinflammatory gene expression. Focusing on intercellular adhesion molecule 1(ICAM)-1 as a model, we identify two ETS binding sites (EBS −118 and −181) within the ICAM-1 promoter required for Erg-mediated repression. We show that Erg binds to both EBS −118 and EBS −181, the latter located within the NF-κB binding site. Interestingly, inhibition of Erg expression in quiescent EC results in increased NF-κB-dependent ICAM-1 expression, indicating that Erg represses basal NF-κB activity. Erg prevents NF-κB p65 from binding to the ICAM-1 promoter, suggesting a direct mechanism of interference. Gene set enrichment analysis of transcriptome profiles of Erg and NF-κB-dependent genes, together with chromatin immunoprecipitation (ChIP) studies, reveals that this mechanism is common to other proinflammatory genes, including cIAP-2 and IL-8. These results identify a role for Erg as a gatekeeper controlling vascular inflammation, thus providing an important barrier to protect against inappropriate endothelial activation.
Birdsey GM, Dryden NH, Shah AV, et al., 2012, The transcription factor Erg regulates expression of histone deacetylase 6 and multiple pathways involved in endothelial cell migration and angiogenesis, BLOOD, Vol: 119, Pages: 894-903, ISSN: 0006-4971
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- Citations: 57
Garonna E, Botham KM, Birdsey GM, et al., 2011, Vascular Endothelial Growth Factor Receptor-2 Couples Cyclo-Oxygenase-2 with Pro-Angiogenic Actions of Leptin on Human Endothelial Cells, PLOS ONE, Vol: 6, ISSN: 1932-6203
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- Citations: 81
Sperone A, Dryden NH, Birdsey GM, et al., 2011, The Transcription Factor Erg Inhibits Vascular Inflammation by Repressing NF-κB Activation and Proinflammatory Gene Expression in Endothelial Cells, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 31, Pages: 142-150, ISSN: 1079-5642
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- Citations: 50
Sperone A, Birdsey GM, Dryden N, et al., 2010, The Transcription Factor Erg Represses ICAM-1 Expression and Vascular Inflammation, Scientific Sessions on Arteriosclerosis, Thrombosis and Vascular Biology, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: E228-E228, ISSN: 1079-5642
Sperone A, Dryden N, Birdsey GM, et al., 2010, The transcription factor Erg represses ICAM-1 expression and vascular inflammation, Publisher: ELSEVIER IRELAND LTD, Pages: E17-E17, ISSN: 0021-9150
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- Citations: 1
McKinnon TA, Goode EC, Birdsey GM, et al., 2010, Specific N-linked glycosylation sites modulate synthesis and secretion of von Willebrand factor, Blood
Hamdulay SS, Wang B, Birdsey GM, et al., 2010, Celecoxib activates PI-3K/Akt and mitochondrial redox signaling to enhance heme oxygenase-1-mediated anti-inflammatory activity in vascular endothelium, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 48, Pages: 1013-1023, ISSN: 0891-5849
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- Citations: 59
Shovlin CL, Angus G, Manning RA, et al., 2010, Endothelial cell processing and alternatively spliced transcripts of factor VIII: potential implications for coagulation cascades and pulmonary hypertension., PLoS One, Vol: 5
BACKGROUND: Coagulation factor VIII (FVIII) deficiency leads to haemophilia A. Conversely, elevated plasma levels are a strong predictor of recurrent venous thromboemboli and pulmonary hypertension phenotypes in which in situ thromboses are implicated. Extrahepatic sources of plasma FVIII are implicated, but have remained elusive. METHODOLOGY/PRINCIPAL FINDINGS: Immunohistochemistry of normal human lung tissue, and confocal microscopy, flow cytometry, and ELISA quantification of conditioned media from normal primary endothelial cells were used to examine endothelial expression of FVIII and coexpression with von Willebrand Factor (vWF), which protects secreted FVIII heavy chain from rapid proteloysis. FVIII transcripts predicted from database mining were identified by RT-PCR and sequencing. FVIII mAb-reactive material was demonstrated in CD31+ endothelial cells in normal human lung tissue, and in primary pulmonary artery, pulmonary microvascular, and dermal microvascular endothelial cells. In pulmonary endothelial cells, this protein occasionally colocalized with vWF, centered on Weibel Palade bodies. Pulmonary artery and pulmonary microvascular endothelial cells secreted low levels of FVIII and vWF to conditioned media, and demonstrated cell surface expression of FVIII and vWF Ab-reacting proteins compared to an isotype control. Four endothelial splice isoforms were identified. Two utilize transcription start sites in alternate 5' exons within the int22h-1 repeat responsible for intron 22 inversions in 40% of severe haemophiliacs. A reciprocal relationship between the presence of short isoforms and full-length FVIII transcript suggested potential splice-switching mechanisms. CONCLUSIONS/SIGNIFICANCE: The pulmonary endothelium is confirmed as a site of FVIII secretion, with evidence of synthesis, cell surface expression, and coexpression with vWF. There is complex alternate transcription initiation from the FVIII gene. These findings provide a framework for future re
Randi AM, Sperone A, Dryden NH, et al., 2009, Regulation of angiogenesis by ETS transcription factors, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 37, Pages: 1248-1253, ISSN: 0300-5127
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- Citations: 59
Sperone A, Birdsey GM, Dryden N, et al., 2009, THE TRANSCRIPTION FACTOR ERG PREVENTS VASCULAR INFLAMMATION BY REPRESSING ICAM-1 EXPRESSION, Spring Meeting of the British-Society-for-Cardiovascular-Research, Publisher: B M J PUBLISHING GROUP, ISSN: 1355-6037
Shovlin CL, Manning R, Angus G, et al., 2008, PULMONARY ENDOTHELIAL CELL SECRETION OF FACTOR VIII, Winter Meeting of the British-Thoracic-Society, Publisher: B M J PUBLISHING GROUP, Pages: A42-A42, ISSN: 0040-6376
Birdsey GM, Dryden NH, Arnsellem V, et al., 2008, The transcription factor Erg regulates angiogenesis and endothelial apoptosis through VE-cadherin, Spring Meeting of the British-Atherosclerosis-Society on Angiogenesis and Atherosclerosis, Publisher: ELSEVIER IRELAND LTD, Pages: 465-465, ISSN: 0021-9150
Birdsey GM, Dryden NH, Amsellem V, et al., 2008, Transcription factor Erg regulates angiogenesis and endothelial apoptosis through VE-cadherin, BLOOD, Vol: 111, Pages: 3498-3506, ISSN: 0006-4971
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- Citations: 195
Randi AM, Birdsey G, Dryden N, et al., 2008, The transcription factor Erg regulates angiogenesis and endothelial apoptosis, FASEB JOURNAL, Vol: 22, ISSN: 0892-6638
Randi AM, Amsellem V, Dryden N, et al., 2008, The adhesion molecule ICAM-2 regulates contact inhibition in endothelial cells, FASEB JOURNAL, Vol: 22, ISSN: 0892-6638
Birdsey GM, Amsellem V, Mason JC, et al., 2007, The transcription factor ERG regulates angiogenesis and apoptosis, Joint Autumn Meeting of the British-Society-for-Cardiovascular-Research/British-Atherosclerosis-Society, Publisher: B M J PUBLISHING GROUP, ISSN: 1355-6037
Garonna E, Birdsey GM, Botham KM, et al., 2006, Leptin-memated proliferation and migration of human endothelial depends on cyclooxygenase-2 [COX-2] activity and requires activation of vascular endothelial growth factor receptor 2 [VEGFR2], 24th Conference of the European-Society-for-Microcirculation, Publisher: KARGER, Pages: 58-58, ISSN: 1018-1172
Huang MT, Mason JC, Birdsey GM, et al., 2005, Endothelial intercellular adhesion molecule (ICAM)-2 regulates angiogenesis, BLOOD, Vol: 106, Pages: 1636-1643, ISSN: 0006-4971
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- Citations: 65
Huber PAJ, Birdsey GM, Lumb MJ, et al., 2005, Peroxisomal import of human alanine:glyoxylate aminotransferase requires ancillary targeting information remote from its C terminus, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 280, Pages: 27111-27120
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- Citations: 32
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