Imperial College London

Professor George K. Christophides

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Infectious Diseases & Immunity
 
 
 
//

Contact

 

+44 (0)20 7594 5342g.christophides

 
 
//

Location

 

6167Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Debalke:2019:10.1186/s13071-019-3414-y,
author = {Debalke, S and Habtewold, T and Duchateau, L and Christophides, G},
doi = {10.1186/s13071-019-3414-y},
journal = {Parasites & Vectors},
title = {The effect of silencing immunity related genes on longevity in a naturally occurring Anopheles arabiensis mosquito population from southwest Ethiopia},
url = {http://dx.doi.org/10.1186/s13071-019-3414-y},
volume = {12},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - BackgroundVector control remains the most important tool to prevent malaria transmission. However, it is now severely constrained by the appearance of physiological and behavioral insecticide resistance. Therefore, the development of new vector control tools is warranted. Such tools could include immunization of blood hosts of vector mosquitoes with mosquito proteins involved in midgut homeostasis (anti-mosquito vaccines) or genetic engineering of mosquitoes that can drive population-wide knockout of genes producing such proteins to reduce mosquito lifespan and malaria transmission probability.MethodsTo achieve this, candidate genes related to midgut homeostasis regulation need to be assessed for their effect on mosquito survival. Here, different such candidate genes were silenced through dsRNA injection in the naturally occurring Anopheles arabiensis mosquitoes and the effect on mosquito survival was evaluated.ResultsSignificantly higher mortality rates were observed in the mosquitoes silenced for FN3D1 (AARA003032), FN3D3 (AARA007751) and GPRGr9 (AARA003963) genes as compared to the control group injected with dsRNA against a non-related bacterial gene (LacZ). This observed difference in mortality rate between the candidate genes and the control disappeared when gene-silenced mosquitoes were treated with antibiotic mixtures, suggesting that gut microbiota play a key role in the observed reduction of mosquito survival.ConclusionsWe demonstrated that interference with the expression of the FN3D1, FN3D3 or GPRGr9 genes causes a significant reduction of the longevity of An. arabiensis mosquito in the wild.
AU - Debalke,S
AU - Habtewold,T
AU - Duchateau,L
AU - Christophides,G
DO - 10.1186/s13071-019-3414-y
PY - 2019///
SN - 1756-3305
TI - The effect of silencing immunity related genes on longevity in a naturally occurring Anopheles arabiensis mosquito population from southwest Ethiopia
T2 - Parasites & Vectors
UR - http://dx.doi.org/10.1186/s13071-019-3414-y
UR - http://hdl.handle.net/10044/1/69701
VL - 12
ER -