Imperial College London

Professor George K. Christophides

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Infectious Diseases & Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5342g.christophides

 
 
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Location

 

6167Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Angrisano:2019:10.1038/s41598-019-54613-0,
author = {Angrisano, F and Sala, K and Tapanelli, S and Christophides, G and Blagborough, A},
doi = {10.1038/s41598-019-54613-0},
journal = {Scientific Reports},
title = {Male-specific protein disulphide isomerase function is essential for plasmodium transmission and a vulnerable target for intervention},
url = {http://dx.doi.org/10.1038/s41598-019-54613-0},
volume = {9},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Inhibiting transmission of Plasmodium is an essential strategy in malaria eradication, and the biological process of gamete fusion during fertilization is a proven target for this approach. Lack of knowledge of the mechanisms underlying fertilization have been a hindrance in the development of transmission-blocking interventions. Here we describe a protein disulphide isomerase essential for malarial transmission (PDI-Trans/PBANKA_0820300) to the mosquito. We show that PDI-Trans activity is male-specific, surface-expressed, essential for fertilization/transmission, and exhibits disulphide isomerase activity which is up-regulated post-gamete activation. We demonstrate that PDI-Trans is a viable anti-malarial drug and vaccine target blocking malarial transmission with the use of PDI inhibitor bacitracin (98.21%/92.48% reduction in intensity/prevalence), and anti-PDI-Trans antibodies (66.22%/33.16% reduction in intensity/prevalence). To our knowledge, these results provide the first evidence that PDI function is essential for malarial transmission, and emphasize the potential of anti-PDI agents to act as anti-malarials, facilitating the future development of novel transmission-blocking interventions.
AU - Angrisano,F
AU - Sala,K
AU - Tapanelli,S
AU - Christophides,G
AU - Blagborough,A
DO - 10.1038/s41598-019-54613-0
PY - 2019///
SN - 2045-2322
TI - Male-specific protein disulphide isomerase function is essential for plasmodium transmission and a vulnerable target for intervention
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/s41598-019-54613-0
UR - http://hdl.handle.net/10044/1/74891
VL - 9
ER -