Imperial College London

Professor George K. Christophides

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Infectious Diseases & Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5342g.christophides

 
 
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Location

 

6167Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Witmer:2020:10.1038/s41598-020-63121-5,
author = {Witmer, K and Fraschka, S and Vlachou, D and Bartfai, R and Christophides, G},
doi = {10.1038/s41598-020-63121-5},
journal = {Scientific Reports},
title = {An epigenetic map of malaria parasite development from host to vector},
url = {http://dx.doi.org/10.1038/s41598-020-63121-5},
volume = {10},
year = {2020}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The malaria parasite replicates asexually in the red blood cells of its vertebrate host employing epigenetic mechanisms to regulate gene expression in response to changes in its environment. We used chromatin immunoprecipitation followed by sequencing in conjunction with RNA sequencing to create an epigenomic and transcriptomic map of the developmental transition from asexual blood stages to male and female gametocytes and to ookinetes in the rodent malaria parasite Plasmodium berghei. Across the developmental stages examined, heterochromatin protein 1 associates with variantly expressed gene families localised at subtelomeric regions and variant gene expression based on heterochromatic silencing is observed only in some genes. Conversely, the euchromatin mark histone 3 lysine 9 acetylation (H3K9ac) is abundant in non-heterochromatic regions across all developmental stages. H3K9ac presents a distinct pattern of enrichment around the start codon of ribosomal protein genes in all stages but male gametocytes. Additionally, H3K9ac occupancy positively correlates with transcript abundance in all stages but female gametocytes suggesting that transcription in this stage is independent of H3K9ac levels. This finding together with known mRNA repression in female gametocytes suggests a multilayered mechanism operating in female gametocytes in preparation for fertilization and zygote development, coinciding with parasite transition from host to vector.
AU - Witmer,K
AU - Fraschka,S
AU - Vlachou,D
AU - Bartfai,R
AU - Christophides,G
DO - 10.1038/s41598-020-63121-5
PY - 2020///
SN - 2045-2322
TI - An epigenetic map of malaria parasite development from host to vector
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/s41598-020-63121-5
UR - http://hdl.handle.net/10044/1/79012
VL - 10
ER -