Imperial College London

Professor George K. Christophides

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Infectious Diseases & Immunity



+44 (0)20 7594 5342g.christophides




6167Sir Alexander Fleming BuildingSouth Kensington Campus






BibTex format

author = {Akorli, J and Gendrin, M and Pels, NAP and Yeboah-Manu, D and Christophides, GK and Wilson, MD},
doi = {10.1371/journal.pone.0157529},
journal = {PLOS One},
title = {Seasonality and Locality Affect the Diversity of Anopheles gambiae and Anopheles coluzzii Midgut Microbiota from Ghana},
url = {},
volume = {11},
year = {2016}

RIS format (EndNote, RefMan)

AB - Symbiotic bacteria can have important implications in the development and competence of disease vectors. In Anopheles mosquitoes, the composition of the midgut microbiota is largely influenced by the larval breeding site, but the exact factors shaping this composition are currently unknown. Here, we examined whether the proximity to urban areas and seasons have an impact on the midgut microbial community of the two major malaria vectors in Africa, An. coluzzii and An. gambiae. Larvae and pupae were collected from selected habitats in two districts of Ghana during the dry and rainy season periods. The midgut microbiota of adults that emerged from these collections was determined by 454-pyrosequencing of the 16S ribosomal DNA. We show that in both mosquito species, Shewanellaceae constituted on average of 54% and 73% of the midgut microbiota from each site in the dry and rainy season, respectively. Enterobacteriaceae was found in comparatively low abundance below 1% in 22/30 samples in the dry season, and in 25/38 samples in the rainy season. Our data indicate that seasonality and locality significantly affect both the diversity of microbiota and the relative abundance of bacterial families with a positive impact of dry season and peri-urban settings.
AU - Akorli,J
AU - Gendrin,M
AU - Pels,NAP
AU - Yeboah-Manu,D
AU - Christophides,GK
AU - Wilson,MD
DO - 10.1371/journal.pone.0157529
PY - 2016///
SN - 1932-6203
TI - Seasonality and Locality Affect the Diversity of Anopheles gambiae and Anopheles coluzzii Midgut Microbiota from Ghana
T2 - PLOS One
UR -
UR -
VL - 11
ER -