Imperial College London
Alternate

Professor George K. Christophides

Faculty of Natural Sciences, Department of Life Sciences

Professor of Infectious Diseases & Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5342g.christophides

 
 
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Location

 

6165Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ruiz:2019:10.1371/journal.pone.0214753,
author = {Ruiz, VMR and Sousa, GL and Sneed, SD and Farrant, KV and Christophides, GK and Povelones, M},
doi = {10.1371/journal.pone.0214753},
journal = {PLoS One},
pages = {1--14},
title = {Stimulation of a protease targeting the LRIM1/APL1C complex reveals specificity in complement-like pathway activation in Anopheles gambiae},
url = {http://dx.doi.org/10.1371/journal.pone.0214753},
volume = {14},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The complement-like pathway of the African malaria mosquito Anopheles gambiae provides protection against infection by diverse pathogens. A functional requirement for a core set of proteins during infections by rodent and human malaria parasites, bacteria, and fungi suggests a similar mechanism operates against different pathogens. However, the extent to which the molecular mechanisms are conserved is unknown. In this study we probed the biochemical responses of complement-like pathway to challenge by the Gram-positive bacterium Staphyloccocus aureus. Western blot analysis of the hemolymph revealed that S. aureus challenge activates a TEP1 convertase-like activity and promotes the depletion of the protein SPCLIP1. S. aureus challenge did not lead to an apparent change in the abundance of the LRIM1/APL1C complex compared to challenge by the Gram-negative bacterium, Escherichia coli. Following up on this observation using a panel of LRIM1 and APL1C antibodies, we found that E. coli challenge, but not S. aureus, specifically activates a protease that cleaves the C-terminus of APL1C. Inhibitor studies in vivo and in vitro protease assays suggest that a serine protease is responsible for APL1C cleavage. This study reveals that despite different challenges converging on activation of a TEP1 convertase-like activity, the mosquito complement-like pathway also includes pathogen-specific reactions.
AU  - Ruiz,VMR
AU  - Sousa,GL
AU  - Sneed,SD
AU  - Farrant,KV
AU  - Christophides,GK
AU  - Povelones,M
DO  - 10.1371/journal.pone.0214753
EP  - 14
PY  - 2019///
SN  - 1932-6203
SP  - 1
TI  - Stimulation of a protease targeting the LRIM1/APL1C complex reveals specificity in complement-like pathway activation in Anopheles gambiae
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0214753
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000463695900020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214753
UR  - http://hdl.handle.net/10044/1/91805
VL  - 14
ER  -
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