Imperial College London
Alternate

Professor George K. Christophides

Faculty of Natural Sciences, Department of Life Sciences

Professor of Infectious Diseases & Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5342g.christophides

 
 
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Location

 

6165Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Debalke:2020:10.1186/s13071-020-04078-2,
author = {Debalke, S and Habtewold, T and Christophides, GK and Duchateau, L},
doi = {10.1186/s13071-020-04078-2},
journal = {Parasites and Vectors},
pages = {1--9},
title = {Stability of the effect of silencing fibronectin type III domain-protein 1 (FN3D1) gene on Anopheles arabiensis reared under different breeding site conditions},
url = {http://dx.doi.org/10.1186/s13071-020-04078-2},
volume = {13},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundMalaria vector mosquitoes acquire midgut microbiota primarily from their habitat. The homeostasis of these microbial communities plays an essential role in the mosquito longevity, the most essential factor in the mosquito vectorial capacity. Our recent study revealed that silencing genes involved in regulation of the midgut homeostasis including FN3D1, FN3D3 and GPRGr9 reduced the survival of female adult Anopheles arabiensis mosquitoes. In the present study, we investigate the stability of the gene silencing efficiency of mosquitoes reared in three different breeding conditions representing distinct larval habitat types: town brick pits in Jimma, flood pools in the rural land of Asendabo and roadside pools in Wolkite.MethodsFirst-instar larvae of An. arabiensis mosquitoes were reared separately using water collected from the three breeding sites. The resulting adult females were micro-injected with dsRNA targeting the FN3D1 gene (AARA003032) and their survival was monitored. Control mosquitoes were injected with dsRNA Lacz. In addition, the load of midgut microbiota of these mosquitoes was determined using flow cytometry.ResultsSurvival of naïve adult female mosquitoes differed between the three sites. Mosquitoes reared using water collected from brick pits and flood pools survived longer than mosquitoes reared using water collected from roadside. However, the FN3D1 gene silencing effect on survival did not differ between the three sites.ConclusionsThe present study revealed that the efficacy of FN3D1 gene silencing is not affected by variation in the larval habitat. Thus, silencing this gene has potential for application throughout sub-Saharan Africa.
AU  - Debalke,S
AU  - Habtewold,T
AU  - Christophides,GK
AU  - Duchateau,L
DO  - 10.1186/s13071-020-04078-2
EP  - 9
PY  - 2020///
SN  - 1756-3305
SP  - 1
TI  - Stability of the effect of silencing fibronectin type III domain-protein 1 (FN3D1) gene on Anopheles arabiensis reared under different breeding site conditions
T2  - Parasites and Vectors
UR  - http://dx.doi.org/10.1186/s13071-020-04078-2
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000529211900003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04078-2
UR  - http://hdl.handle.net/10044/1/91687
VL  - 13
ER  -
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