Imperial College London
Alternate

Professor George K. Christophides

Faculty of Natural Sciences, Department of Life Sciences

Professor of Infectious Diseases & Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5342g.christophides

 
 
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Location

 

6165Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ukegbu:2023:10.1101/2023.03.14.532540,
author = {Ukegbu, CV and Gomes, AR and Giorgalli, M and Campos, M and Bailey, AJ and Besson, TRB and Billker, O and Vlachou, D and Christophides, GK},
doi = {10.1101/2023.03.14.532540},
title = {Reverse genetic screen identifies malaria parasite genes required for gametocyte-to-sporozoite development in its mosquito host},
url = {http://dx.doi.org/10.1101/2023.03.14.532540},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Summary</jats:title><jats:p>Malaria remains one of the most devastating infectious diseases. Reverse genetic screens offer a powerful approach to identify genes and molecular processes governing malaria parasite biology. However, sexual reproduction and complex regulation of gene expression and genotype-phenotype associations in the mosquito have hampered the development of screens in this key part of the parasite lifecycle. We designed a genetic approach in the rodent parasite<jats:italic>Plasmodium berghei</jats:italic>, which in conjunction with barcode sequencing allowed us to overcome the fertilization roadblock and screen for gametocyte-expressed genes required for parasite infection of the mosquito<jats:italic>Anopheles coluzzii</jats:italic>. The results confirmed previous findings, validating our approach for scaling up, and identified new genes required for ookinete motility and mosquito midgut infection and for sporozoite development and oocyst egress and salivary gland infection. Our findings can assist efforts to study malaria transmission biology and develop new interventions to control disease transmission.</jats:p>
AU  - Ukegbu,CV
AU  - Gomes,AR
AU  - Giorgalli,M
AU  - Campos,M
AU  - Bailey,AJ
AU  - Besson,TRB
AU  - Billker,O
AU  - Vlachou,D
AU  - Christophides,GK
DO  - 10.1101/2023.03.14.532540
PY  - 2023///
TI  - Reverse genetic screen identifies malaria parasite genes required for gametocyte-to-sporozoite development in its mosquito host
UR  - http://dx.doi.org/10.1101/2023.03.14.532540
ER  -
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