Imperial College London
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ProfessorGrahamCooke

Faculty of Medicine, Department of Infectious Disease

Vice Dean (Research); Professor of Infectious Diseases
 
 
 
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Contact

 

g.cooke

 
 
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Location

 

Infectious Diseases SectionMedical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Khan:2022:10.1038/s41598-022-05640-x,
author = {Khan, M and Rosadas, C and Katsanovskaja, K and Weber, ID and Shute, J and Ijaz, S and Marchesin, F and McClure, E and Elias, S and Flower, B and Gao, H and Quinlan, R and Short, C and Rosa, A and Roustan, C and Moshe, M and Taylor, GP and Elliott, P and Cooke, GS and Cherepanov, P and Parker, E and McClure, MO and Tedder, RS},
doi = {10.1038/s41598-022-05640-x},
journal = {Scientific Reports},
title = {Simple, sensitive, specific self-sampling assay secures SARS-CoV-2 antibody signals in sero-prevalence and post-vaccine studies},
url = {http://dx.doi.org/10.1038/s41598-022-05640-x},
volume = {12},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - At-home sampling is key to large scale seroprevalence studies. Dried blood spot (DBS) self-sampling removes the need for medical personnel for specimen collection but facilitates specimen referral to an appropriately accredited laboratory for accurate sample analysis. To establish a highly sensitive and specific antibody assay that would facilitate self-sampling for prevalence and vaccine-response studies. Paired sera and DBS eluates collected from 439 sero-positive, 382 sero-negative individuals and DBS from 34 vaccine recipients were assayed by capture ELISAs for IgG and IgM antibody to SARS-CoV-2. IgG and IgM combined on DBS eluates achieved a diagnostic sensitivity of 97.9% (95%CI 96.6 to 99.3) and a specificity of 99.2% (95% CI 98.4 to 100) compared to serum, displaying limits of detection equivalent to 23 and 10 WHO IU/ml, respectively. A strong correlation (r = 0.81) was observed between serum and DBS reactivities. Reactivity remained stable with samples deliberately rendered inadequate, (p = 0.234) and when samples were accidentally damaged or 'invalid'. All vaccine recipients were sero-positive. This assay provides a secure method for self-sampling by DBS with a sensitivity comparable to serum. The feasibility of DBS testing in sero-prevalence studies and in monitoring post-vaccine responses was confirmed, offering a robust and reliable tool for serological monitoring at a population level.
AU  - Khan,M
AU  - Rosadas,C
AU  - Katsanovskaja,K
AU  - Weber,ID
AU  - Shute,J
AU  - Ijaz,S
AU  - Marchesin,F
AU  - McClure,E
AU  - Elias,S
AU  - Flower,B
AU  - Gao,H
AU  - Quinlan,R
AU  - Short,C
AU  - Rosa,A
AU  - Roustan,C
AU  - Moshe,M
AU  - Taylor,GP
AU  - Elliott,P
AU  - Cooke,GS
AU  - Cherepanov,P
AU  - Parker,E
AU  - McClure,MO
AU  - Tedder,RS
DO  - 10.1038/s41598-022-05640-x
PY  - 2022///
SN  - 2045-2322
TI  - Simple, sensitive, specific self-sampling assay secures SARS-CoV-2 antibody signals in sero-prevalence and post-vaccine studies
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-022-05640-x
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35115570
UR  - http://hdl.handle.net/10044/1/94503
VL  - 12
ER  -
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