Imperial College London

ProfessorGadFrankel

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Molecular Pathogenesis
 
 
 
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Contact

 

+44 (0)20 7594 5253g.frankel

 
 
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Location

 

1.46Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

244 results found

Younis R, Bingle LEH, Rollauer S, Munera D, Busby SJ, Johnson S, Deane JE, Lea SM, Frankel G, Pallen MJet al., 2010, SepL Resembles an Aberrant Effector in Binding to a Class 1 Type III Secretion Chaperone and Carrying an N-Terminal Secretion Signal, JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 6093-6098, ISSN: 0021-9193

Journal article

Vossenkaemper A, Marches O, Fairclough PD, Warnes G, Stagg AJ, Lindsay JO, Evans PC, Luong LA, Croft NM, Naik S, Frankel G, MacDonald TTet al., 2010, Inhibition of NF-kappa B Signaling in Human Dendritic Cells by the Enteropathogenic Escherichia coli Effector Protein NleE, JOURNAL OF IMMUNOLOGY, Vol: 185, Pages: 4118-4127, ISSN: 0022-1767

Journal article

Berger CN, Sodha SV, Shaw RK, Griffin PM, Pink D, Hand P, Frankel Get al., 2010, Fresh fruit and vegetables as vehicles for the transmission of human pathogens, ENVIRONMENTAL MICROBIOLOGY, Vol: 12, Pages: 2385-2397, ISSN: 1462-2912

Journal article

Deacon V, Dziva F, van Diemen PM, Frankel G, Stevens MPet al., 2010, Efa-1/LifA mediates intestinal colonization of calves by enterohaemorrhagic Escherichia coli 026: H- in a manner independent of glycosyltransferase and cysteine protease motifs or effects on type III secretion, MICROBIOLOGY-SGM, Vol: 156, Pages: 2527-2536, ISSN: 1350-0872

Journal article

Munera D, Crepin VF, Marches O, Frankel Get al., 2010, N-Terminal Type III Secretion Signal of Enteropathogenic Escherichia coli Translocator Proteins, JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 3534-3539, ISSN: 0021-9193

Journal article

Mitomo K, Griesenbach U, Inoue M, Somerton L, Meng C, Akiba E, Tabata T, Ueda Y, Frankel GM, Farley R, Singh C, Chan M, Munkonge F, Brum A, Xenariou S, Escudero-Garcia S, Hasegawa M, Alton EWFWet al., 2010, Toward Gene Therapy for Cystic Fibrosis Using a Lentivirus Pseudotyped With Sendai Virus Envelopes, MOLECULAR THERAPY, Vol: 18, Pages: 1173-1182, ISSN: 1525-0016

Journal article

Robinson KS, Mousnier A, Hemrajani C, Fairweather N, Berger CN, Frankel Get al., 2010, The enteropathogenic Escherichia coli effector NleH inhibits apoptosis induced by Clostridium difficile toxin B, MICROBIOLOGY-SGM, Vol: 156, Pages: 1815-1823, ISSN: 1350-0872

Journal article

Newton HJ, Pearson JS, Badea L, Kelly M, Lucas M, Holloway G, Wagstaff KM, Dunstone MA, Sloan J, Whisstock JC, Kaper JB, Robins-Browne RM, Jans DA, Frankel G, Phillips AD, Coulson BS, Hartland ELet al., 2010, The Type III Effectors NleE and NleB from Enteropathogenic E. coli and OspZ from Shigella Block Nuclear Translocation of NF-kappa B p65, PLOS PATHOGENS, Vol: 6, ISSN: 1553-7366

Journal article

Arbeloa A, Garnett J, Lillington J, Bulgin RR, Berger CN, Lea SM, Matthews S, Frankel Get al., 2010, EspM2 is a RhoA guanine nucleotide exchange factor, Cellular Microbiology, Vol: 12, Pages: 654-664, ISSN: 1462-5814

We investigated how the type III secretion system WxxxE effectors EspM2 of enterohaemorrhagic Escherichia coli, which triggers stress fibre formation, and SifA of Salmonella enterica serovar Typhimurium, which is involved in intracellular survival, modulate Rho GTPases. We identified a direct interaction between EspM2 or SifA and nucleotide‐free RhoA. Nuclear Magnetic Resonance Spectroscopy revealed that EspM2 has a similar fold to SifA and the guanine nucleotide exchange factor (GEF) effector SopE. EspM2 induced nucleotide exchange in RhoA but not in Rac1 or H‐Ras, while SifA induced nucleotide exchange in none of them. Mutating W70 of the WxxxE motif or L118 and I127 residues, which surround the catalytic loop, affected the stability of EspM2. Substitution of Q124, located within the catalytic loop of EspM2, with alanine, greatly attenuated the RhoA GEF activity in vitro and the ability of EspM2 to induce stress fibres upon ectopic expression. These results suggest that binding of SifA to RhoA does not trigger nucleotide exchange while EspM2 is a unique Rho GTPase GEF.

Journal article

Bulgin R, Raymond B, Garnett JA, Frankel G, Crepin VF, Berger CN, Arbeloa Aet al., 2010, Bacterial Guanine Nucleotide Exchange Factors SopE-Like and WxxxE Effectors, INFECTION AND IMMUNITY, Vol: 78, Pages: 1417-1425, ISSN: 0019-9567

Journal article

Hemrajani C, Berger CN, Robinson KS, Marches O, Mousnier A, Frankel Get al., 2010, NleH effectors interact with Bax inhibitor-1 to block apoptosis during enteropathogenic Escherichia coli infection, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 3129-3134, ISSN: 0027-8424

Journal article

Chaudhuri RR, Sebaihia M, Hobman JL, Webber MA, Leyton DL, Goldberg MD, Cunningham AF, Scott-Tucker A, Ferguson PR, Thomas CM, Frankel G, Tang CM, Dudley EG, Roberts IS, Rasko DA, Pallen MJ, Parkhill J, Nataro JP, Thomson NR, Henderson IRet al., 2010, Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042, PLOS ONE, Vol: 5, ISSN: 1932-6203

Journal article

Crepin VF, Girard F, Schueller S, Phillips AD, Mousnier A, Frankel Get al., 2010, Dissecting the role of the Tir:Nck and Tir:IRTKS/IRSp53 signalling pathways in vivo, MOLECULAR MICROBIOLOGY, Vol: 75, Pages: 308-323, ISSN: 0950-382X

Journal article

Bulgin R, Arbeloa A, Goulding D, Dougan G, Crepin VF, Raymond B, Frankel Get al., 2009, The T3SS Effector EspT Defines a New Category of Invasive Enteropathogenic E. coli (EPEC) Which Form Intracellular Actin Pedestals, PLOS PATHOGENS, Vol: 5, ISSN: 1553-7366

Journal article

Petty NK, Bulgin R, Crepin VF, Cerdeno-Tarraga AM, Schroeder GN, Quail MA, Lennard N, Corton C, Barron A, Clark L, Toribio AL, Parkhill J, Dougan G, Frankel G, Thomson NRet al., 2009, The Citrobacter rodentium Genome Sequence Reveals Convergent Evolution with Human Pathogenic Escherichia coli, Journal of Bacteriology, Vol: 192, Pages: 525-538, ISSN: 1098-5530

Citrobacter rodentium (formally Citrobacter freundii biotype 4280) is a highly infectious pathogen that causes colitis and transmissible colonic hyperplasia in mice. In common with enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), C. rodentium exploits a type III secretion system (T3SS) to induce attaching and effacing (A/E) lesions that are essential for virulence. Here, we report the fully annotated genome sequence of the 5.3-Mb chromosome and four plasmids harbored by C. rodentium strain ICC168. The genome sequence revealed key information about the phylogeny of C. rodentium and identified 1,585 C. rodentium-specific (without orthologues in EPEC or EHEC) coding sequences, 10 prophage-like regions, and 17 genomic islands, including the locus for enterocyte effacement (LEE) region, which encodes a T3SS and effector proteins. Among the 29 T3SS effectors found in C. rodentium are all 22 of the core effectors of EPEC strain E2348/69. In addition, we identified a novel C. rodentium effector, named EspS. C. rodentium harbors two type VI secretion systems (T6SS) (CTS1 and CTS2), while EHEC contains only one T6SS (EHS). Our analysis suggests that C. rodentium and EPEC/EHEC have converged on a common host infection strategy through access to a common pool of mobile DNA and that C. rodentium has lost gene functions associated with a previous pathogenic niche.

Journal article

Frankel G, Shaw RK, Pink D, Berger CN, Hand Pet al., 2009, Fresh produce as a potential vector for bacterial human pathogens, MICROBIAL BIOTECHNOLOGY, Vol: 2, Pages: 595-597, ISSN: 1751-7915

Journal article

Girard F, Dziva F, Stevens MP, Frankel Get al., 2009, Interactions of Typical and Atypical Enteropathogenic Escherichia coli Strains with the Calf Intestinal Mucosa Ex Vivo, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol: 75, Pages: 5991-5995, ISSN: 0099-2240

Journal article

Arbeloa A, Blanco M, Moreira FC, Bulgin R, Lopez C, Dahbi G, Blanco JE, Mora A, Pilar Alonso M, Ceferina Mamani R, Gomes TAT, Blanco J, Frankel Get al., 2009, Distribution of espM and espT among enteropathogenic and enterohaemorrhagic Escherichia coli, JOURNAL OF MEDICAL MICROBIOLOGY, Vol: 58, Pages: 988-995, ISSN: 0022-2615

Journal article

Berger CN, Shaw RK, Ruiz-Perez F, Nataro JP, Henderson IR, Pallen MJ, Frankel Get al., 2009, Interaction of enteroaggregative Escherichia coli with salad leaves, ENVIRONMENTAL MICROBIOLOGY REPORTS, Vol: 1, Pages: 234-239, ISSN: 1758-2229

Journal article

Griesenbach U, Meng C, Farley R, Gardner A, Brake MA, Frankel GM, Gruenert DC, Cheng SH, Scheule RK, Alton EWFWet al., 2009, The role of doxorubicin in non-viral gene transfer in the lung, BIOMATERIALS, Vol: 30, Pages: 1971-1977, ISSN: 0142-9612

Journal article

Girard F, Crepin VF, Frankel G, 2009, Modelling of Infection by Enteropathogenic Escherichia coli Strains in Lineages 2 and 4 Ex Vivo and In Vivo by Using Citrobacter rodentium Expressing TccP, INFECTION AND IMMUNITY, Vol: 77, Pages: 1304-1314, ISSN: 0019-9567

Journal article

Berger CN, Shaw RK, Brown DJ, Mather H, Clare S, Dougan G, Pallen MJ, Frankel Get al., 2009, Interaction of Salmonella enterica with basil and other salad leaves, ISME JOURNAL, Vol: 3, Pages: 261-265, ISSN: 1751-7362

Journal article

Bulgin RR, Arbeloa A, Chung JCS, Frankel Get al., 2009, EspT triggers formation of lamellipodia and membrane ruffles through activation of Rac-1 and Cdc42, CELLULAR MICROBIOLOGY, Vol: 11, Pages: 217-229, ISSN: 1462-5814

Journal article

Berger CN, Crepin VF, Jepson MA, Arbeloa A, Frankel Get al., 2009, The mechanisms used by enteropathogenic Escherichia coli to control filopodia dynamics, CELLULAR MICROBIOLOGY, Vol: 11, Pages: 309-322, ISSN: 1462-5814

Journal article

Iguchi A, Thomson NR, Ogura Y, Saunders D, Ooka T, Henderson IR, Harris D, Asadulghani M, Kurokawa K, Dean P, Kenny B, Quail MA, Thurston S, Dougan G, Hayashi T, Parkhill J, Frankel Get al., 2009, Complete Genome Sequence and Comparative Genome Analysis of Enteropathogenic Escherichia coli O127:H6 Strain E2348/69, JOURNAL OF BACTERIOLOGY, Vol: 191, Pages: 347-354, ISSN: 0021-9193

Journal article

Wiles S, Robertson BD, Frankel G, Kerton Aet al., 2009, Bioluminescent monitoring of in vivo colonization and clearance dynamics by light-emitting bacteria., Methods Mol Biol, Vol: 574, Pages: 137-153

Bioluminescence is an excellent reporter system for analysing bacterial colonization and clearance dynamics in vivo. Many bacterial species have been rendered bioluminescent, allowing the sensitive detection of bacterial burden and metabolic activity in real-time and in situ in living animals. In this chapter we describe the protocols for characterizing in vivo infection models using bioluminescent bacteria: from real-time imaging in living animals by bioluminescence imaging (BLI) to ex vivo BLI of harvested organs and tissues and, finally, to quantification of bacterial numbers in organ and tissue homogenates by luminometry and viable counts. While the lux operon from Photorhabdus luminescens is ideally suited for use in such models, there may be times when alternative luciferases, such as those from the firefly (luc) or marine copepods (Gluc), may be more appropriate. Here we describe the protocols required to monitor colonization and clearance dynamics using bioluminescent bacteria that are lux-, luc-, or Gluc-positive.

Journal article

Dennis A, Kudo T, Kruidenier L, Girard F, Crepin VF, MacDonald TT, Frankel G, Wiles Set al., 2008, The p50 Subunit of NF-kappa B Is Critical for In Vivo Clearance of the Noninvasive Enteric Pathogen Citrobacter rodentium, INFECTION AND IMMUNITY, Vol: 76, Pages: 4978-4988, ISSN: 0019-9567

Journal article

Hart E, Yang J, Tauschek M, Kelly M, Wakefield MJ, Frankel G, Hartland EL, Robins-Browne RMet al., 2008, RegA, an AraC-Like Protein, Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in Citrobacter rodentium, INFECTION AND IMMUNITY, Vol: 76, Pages: 5247-5256, ISSN: 0019-9567

Journal article

Hemrajani C, Marches O, Wiles S, Girard F, Dennis A, Dziva F, Best A, Phillips AD, Berger CN, Mousnier A, Crepin VF, Kruidenier L, Woodward MJ, Stevens MP, La Ragione RM, MacDonald TT, Frankel Get al., 2008, Role of NleH, a Type III Secreted Effector from Attaching and Effacing Pathogens, in Colonization of the Bovine, Ovine, and Murine Gut, INFECTION AND IMMUNITY, Vol: 76, Pages: 4804-4813, ISSN: 0019-9567

Journal article

Mousnier A, Whale AD, Schuller S, Leong JM, Phillips AD, Frankel Get al., 2008, Cortactin recruitment by enterohemorrhagic Escherichia coli O157 : H7 during infection in vitro and ex vivo, INFECTION AND IMMUNITY, Vol: 76, Pages: 4669-4676, ISSN: 0019-9567

Journal article

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