Imperial College London
Portrait Picture

ProfessorGadFrankel

Faculty of Natural Sciences, Department of Life Sciences

Professor of Molecular Pathogenesis
 
 
 
//

Contact

 

+44 (0)20 7594 5253g.frankel

 
 
//

Location

 

1.46Flowers buildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Cepeda-Molero:2017:10.1371/journal.ppat.1006706,
author = {Cepeda-Molero, M and Berger, CN and Walsham, ADS and Ellis, SJ and Wemyss-Holden, S and Schueller, S and Frankel, G and Angel, Fernandez L},
doi = {10.1371/journal.ppat.1006706},
journal = {PLoS Pathogens},
title = {Attaching and effacing (A/E) lesion formation by enteropathogenic E. coli on human intestinal mucosa is dependent on non-LEE effectors},
url = {http://dx.doi.org/10.1371/journal.ppat.1006706},
volume = {13},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Enteropathogenic E. coli (EPEC) is a human pathogen that causes acute and chronic pediatric diarrhea. The hallmark of EPEC infection is the formation of attaching and effacing (A/E) lesions in the intestinal epithelium. Formation of A/E lesions is mediated by genes located on the pathogenicity island locus of enterocyte effacement (LEE), which encode the adhesin intimin, a type III secretion system (T3SS) and six effectors, including the essential translocated intimin receptor (Tir). Seventeen additional effectors are encoded by genes located outside the LEE, in insertion elements and prophages. Here, using a stepwise approach, we generated an EPEC mutant lacking the entire effector genes (EPEC0) and intermediate mutants. We show that EPEC0 contains a functional T3SS. An EPEC mutant expressing intimin but lacking all the LEE effectors but Tir (EPEC1) was able to trigger robust actin polymerization in HeLa cells and mucin-producing intestinal LS174T cells. However, EPEC1 was unable to form A/E lesions on human intestinal in vitro organ cultures (IVOC). Screening the intermediate mutants for genes involved in A/E lesion formation on IVOC revealed that strains lacking non-LEE effector/s have a marginal ability to form A/E lesions. Furthermore, we found that Efa1/LifA proteins are important for A/E lesion formation efficiency in EPEC strains lacking multiple effectors. Taken together, these results demonstrate the intricate relationships between T3SS effectors and the essential role non-LEE effectors play in A/E lesion formation on mucosal surfaces.
AU  - Cepeda-Molero,M
AU  - Berger,CN
AU  - Walsham,ADS
AU  - Ellis,SJ
AU  - Wemyss-Holden,S
AU  - Schueller,S
AU  - Frankel,G
AU  - Angel,Fernandez L
DO  - 10.1371/journal.ppat.1006706
PY  - 2017///
SN  - 1553-7366
TI  - Attaching and effacing (A/E) lesion formation by enteropathogenic E. coli on human intestinal mucosa is dependent on non-LEE effectors
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1006706
UR  - http://hdl.handle.net/10044/1/56472
VL  - 13
ER  -
Download