Imperial College London
Portrait Picture

Professor Guido Franzoso

Faculty of Medicine, Department of Immunology and Inflammation

Chair in Inflammation and Signal Transduction
 
 
 
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Contact

 

+44 (0)20 3313 8421g.franzoso Website

 
 
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Assistant

 

Miss Anjli Jagpal +44 (0)20 3313 3152

 
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Location

 

5N1Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Luo:2011:10.1002/art.30497,
author = {Luo, Y and Boyle, DL and Hammaker, D and Edgar, M and Franzoso, G and Firestein, GS},
doi = {10.1002/art.30497},
journal = {Arthritis and Rheumatism},
pages = {2949--2955},
title = {Suppression of collagen-induced arthritis in growth arrest and DNA damage-inducible protein 45 beta-deficient mice},
url = {http://dx.doi.org/10.1002/art.30497},
volume = {63},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ObjectiveGrowth arrest and DNA damage–inducible protein 45β (GADD45β) is involved in stress responses, cell cycle regulation, and oncogenesis. Previous studies demonstrated that GADD45β deficiency exacerbates K/BxN serum–induced arthritis and experimental allergic encephalomyelitis (EAE) in mice, indicating that GADD45β plays a suppressive role in innate and adaptive immune responses. To further understand how GADD45β regulates autoimmunity, we evaluated collagen-induced arthritis in GADD45β–/– mice.MethodsWild-type (WT) and GADD45β–/– DBA/1 mice were immunized with bovine type II collagen (CII). Serum anticollagen antibody levels were quantified by enzyme-linked immunosorbent assay. Expression of cytokines and matrix metalloproteinases in the joint and spleen was determined by quantitative polymerase chain reaction. The in vitro T cell cytokine response to CII was measured by multiplex analysis. CD4+CD25+ Treg cells and Th17 cells were quantified using flow cytometry.ResultsGADD45β–/– mice showed significantly lower arthritis severity and joint destruction compared with WT mice. MMP-3 and MMP-13 expression was also markedly reduced in GADD45β–/– mice. However, serum anti-CII antibody levels were similar in both groups. FoxP3 and interleukin-10 (IL-10) expression was increased 2–3-fold in splenocytes from arthritic GADD45β–/– mice compared with those from WT mice. Flow cytometric analysis showed greater numbers of CD4+CD25+ Treg cells in the spleen of GADD45β–/– mice than in the spleen of WT mice. In vitro studies showed that interferon-γ and IL-17 production by T cells was significantly decreased in GADD45β–/– mice.ConclusionUnlike passive K/BxN arthritis and EAE, GADD45β deficiency in CIA was associated with lower arthritis severity, elevated IL-10 expression, decreased IL-17 production, and in
AU  - Luo,Y
AU  - Boyle,DL
AU  - Hammaker,D
AU  - Edgar,M
AU  - Franzoso,G
AU  - Firestein,GS
DO  - 10.1002/art.30497
EP  - 2955
PY  - 2011///
SN  - 0004-3591
SP  - 2949
TI  - Suppression of collagen-induced arthritis in growth arrest and DNA damage-inducible protein 45 beta-deficient mice
T2  - Arthritis and Rheumatism
UR  - http://dx.doi.org/10.1002/art.30497
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000295293000013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/10.1002/art.30497
UR  - http://hdl.handle.net/10044/1/99769
VL  - 63
ER  -
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