Imperial College London

Giorgio F. Gilestro

Faculty of Natural SciencesDepartment of Life Sciences

Senior Lecturer
 
 
 
//

Contact

 

+44 (0)20 7594 5443g.gilestro Website

 
 
//

Location

 

408Sir Ernst Chain BuildingSouth Kensington Campus

//

Summary

 

Publications

Publication Type
Year
to

15 results found

Geissmann Q, Beckwith E, Gilestro G, 2019, Most sleep does not serve a vital function. Evidence from Drosophila melanogaster, Science Advances, Vol: 5, ISSN: 2375-2548

Sleep appears to be a universally conserved phenomenon among the animal kingdom but whether this striking evolutionary conservation underlies a basic vital function is still an open question. Using a machine-learning based video-tracking technology, we conducted a detailed high-throughput analysis of sleep in the fruit fly Drosophila melanogaster,coupled with a life-long chronic and specific sleep restriction. Our results show that some wild-type flies are virtually sleepless in baseline conditions and that complete, forced sleep restriction is not necessarily a lethal treatment in wild-type Drosophila melanogaster. We also show that circadian drive, and not homeostatic regulation, is the main contributor to sleep pressure in flies. These results offer a new perspective on the biological role of sleep in Drosophilaand, potentially, in other species.

Journal article

Geissmann Q, Garcia Rrodriguez L, Beckwith E, Gilestro Get al., 2019, Rethomics: an R framework to analyse high-throughput behavioural data, PLoS ONE, Vol: 14, ISSN: 1932-6203

The recent development of automatised methods to score various behaviours on a large number of animals provides biologists with an unprecedented set of tools to decipher these complex phenotypes. Analysing such data comes with several challenges that are largely shared across acquisition platform and paradigms. Here, we present rethomics, a set of R packages that unifies the analysis of behavioural datasets in an efficient and flexible manner. rethomics offers a computational solution to storing, manipulating and visualising large amounts of behavioural data. We propose it as a tool to bridge the gap between behavioural biology and data sciences, thus connecting computational and behavioural scientists. rethomics comes with a extensive documentation as well as a set of both practical and theoretical tutorials (available at https://rethomics.github.io).

Journal article

Geissmann Q, Garcia Rodriguez L, Beckwith EJ, French AS, Jamasb AR, Gilestro GFet al., 2017, Ethoscopes: An open platform for high-throughput ethomics., PLoS Biology, Vol: 15, ISSN: 1544-9173

Here, we present the use of ethoscopes, which are machines for high-throughput analysis of behavior in Drosophila and other animals. Ethoscopes provide a software and hardware solution that is reproducible and easily scalable. They perform, in real-time, tracking and profiling of behavior by using a supervised machine learning algorithm, are able to deliver behaviorally triggered stimuli to flies in a feedback-loop mode, and are highly customizable and open source. Ethoscopes can be built easily by using 3D printing technology and rely on Raspberry Pi microcomputers and Arduino boards to provide affordable and flexible hardware. All software and construction specifications are available at http://lab.gilest.ro/ethoscope.

Journal article

Beckwith EJ, Geissmann Q, French AS, Gilestro GFet al., 2017, Regulation of sleep homeostasis by sexual arousal., eLife, Vol: 6, ISSN: 2050-084X

In all animals, sleep pressure is under continuous tight regulation. It is universally accepted that this regulation arises from a two-process model, integrating both a circadian and a homeostatic controller. Here we explore the role of environmental social signals as a third, parallel controller of sleep homeostasis and sleep pressure. We show that, in Drosophila melanogaster males, sleep pressure after sleep deprivation can be counteracted by raising their sexual arousal, either by engaging the flies with prolonged courtship activity or merely by exposing them to female pheromones.

Journal article

Gilestro GF, 2012, Video tracking and analysis of sleep in Drosophila melanogaster, NATURE PROTOCOLS, Vol: 7, Pages: 995-1007, ISSN: 1754-2189

Journal article

Gilestro GF, Cirelli C, 2010, pySolo

pySolo is a multiplatform software for analysis of sleep and locomotor activity in Drosophila melanogaster. pySolo provides a user-friendly graphic interface and it has been developed with the specific aim of being accessible, portable, fast and easily expandable through an intuitive plug-in structure. Support for development of additional plug-ins is provided through a community website. AVAILABILITY: Software and documentation are located at (http://www.pysolo.net). pySolo is a free software released under the GNU General Public License.

Software

Gilestro GF, Cirelli C, 2009, pySolo: a complete suite for sleep analysis in Drosophila, BIOINFORMATICS, Vol: 25, Pages: 1466-1467, ISSN: 1367-4803

Journal article

Gilestro GF, Tononi G, Cirelli C, 2009, Widespread Changes in Synaptic Markers as a Function of Sleep and Wakefulness in Drosophila, SCIENCE, Vol: 324, Pages: 109-112, ISSN: 0036-8075

Journal article

Gilestro GF, 2008, Redundant Mechanisms for Regulation of Midline Crossing in Drosophila, PLOS ONE, Vol: 3, ISSN: 1932-6203

Journal article

Dickson BJ, Gilestro GF, 2006, Regulation of commissural axon pathfinding by slit and its robo receptors, ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, Vol: 22, Pages: 651-675, ISSN: 1081-0706

Journal article

Christensen C, Ambartsumian N, Gilestro G, Thomsen B, Comoglio P, Tamagnone L, Guldberg P, Lukanidin Eet al., 2005, Proteolytic processing converts the repelling signal Sema3E into an inducer of invasive growth and lung metastasis, CANCER RESEARCH, Vol: 65, Pages: 6167-6177, ISSN: 0008-5472

Journal article

Artigiani S, Conrotto P, Fazzari P, Gilestro GF, Barberis D, Giordano S, Comoglio PM, Tamagnone Let al., 2004, Plexin-B3 is a functional receptor for semaphorin 5A, EMBO REPORTS, Vol: 5, Pages: 710-714, ISSN: 1469-221X

Journal article

Giordano S, Corso S, Conrotto P, Artigiani S, Gilestro G, Barberis D, Tamagnone L, Comoglio PMet al., 2002, The Semaphorin 4D receptor controls invasive growth by coupling with Met, NATURE CELL BIOLOGY, Vol: 4, Pages: 720-724, ISSN: 1465-7392

Journal article

Petrelli A, Gilestro GF, Lanzardo S, Comoglio PM, Migone N, Giordano Set al., 2002, The endophilin-CIN85-Cbl complex mediates ligand-dependent downregulation of c-Met, NATURE, Vol: 416, Pages: 187-190, ISSN: 0028-0836

Journal article

Geissmann Q, Beckwith EJ, Gilestro GF, Most sleep does not serve a vital function. Evidence from Drosophila melanogaster, Publisher: Cold Spring Harbor Laboratory

<jats:title>Abstract</jats:title><jats:p>Sleep appears to be a universally conserved phenomenon among the animal kingdom but whether this striking evolutionary conservation underlies a basic vital function is still an open question. Using novel technologies, we conducted an unprecedentedly detailed high-throughput analysis of sleep in the fruit fly <jats:italic>Drosophila melanogaster</jats:italic>, coupled with a life-long chronic and specific sleep restriction. Our results show that some wild-type flies are virtually sleepless in baseline conditions and that complete, forced sleep restriction is not necessarily a lethal treatment in wild-type <jats:italic>Drosophila melanogaster</jats:italic>. We also show that circadian drive, and not homeostatic regulation, is the main contributor to sleep pressure in flies. We propose a three-partite model framework of sleep function, according to which, total sleep accounts for three components: a vital component, a useful component, and an accessory component.</jats:p>

Working paper

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.

Request URL: http://wlsprd.imperial.ac.uk:80/respub/WEB-INF/jsp/search-html.jsp Request URI: /respub/WEB-INF/jsp/search-html.jsp Query String: respub-action=search.html&id=00668960&limit=30&person=true