Imperial College London
Alternate

ProfessorGeorgiosKassiotis

Faculty of Medicine, Department of Infectious Disease

Professor of Retrovirology
 
 
 
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Contact

 

g.kassiotis Website

 
 
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Location

 

Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ng:2020:10.1126/science.abe1107,
author = {Ng, KW and Faulkner, N and Cornish, GH and Rosa, A and Harvey, R and Hussain, S and Ufferts, R and Earl, C and Wrobel, AG and Benton, DJ and Roustan, C and Bolland, W and Thompson, R and Agua-Doce, A and Hobson, P and Heaney, J and Rickman, H and Paraskevopoulou, S and Houlihan, CF and Thomson, K and Sanchez, E and Shin, GY and Spyer, MJ and Joshi, D and O'Reilly, N and Walker, PA and Kjaer, S and Riddell, A and Moore, C and Jebson, BR and Wilkinson, M and Marshall, LR and Rosser, EC and Radziszewska, A and Peckham, H and Ciurtin, C and Wedderburn, LR and Beale, R and Swanton, C and Gandhi, S and Stockinger, B and McCauley, J and Gambill, SJ and McCoy, LE and Cherepanov, P and Nastouli, E and Kassiotis, G},
doi = {10.1126/science.abe1107},
journal = {Science},
pages = {1339--1343},
title = {Preexisting and de novo humoral immunity to SARS-CoV-2 in humans},
url = {http://dx.doi.org/10.1126/science.abe1107},
volume = {370},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)–reactive antibodies were detectable using a flow cytometry–based method in SARS-CoV-2–uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S–reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2–uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.
AU  - Ng,KW
AU  - Faulkner,N
AU  - Cornish,GH
AU  - Rosa,A
AU  - Harvey,R
AU  - Hussain,S
AU  - Ufferts,R
AU  - Earl,C
AU  - Wrobel,AG
AU  - Benton,DJ
AU  - Roustan,C
AU  - Bolland,W
AU  - Thompson,R
AU  - Agua-Doce,A
AU  - Hobson,P
AU  - Heaney,J
AU  - Rickman,H
AU  - Paraskevopoulou,S
AU  - Houlihan,CF
AU  - Thomson,K
AU  - Sanchez,E
AU  - Shin,GY
AU  - Spyer,MJ
AU  - Joshi,D
AU  - O'Reilly,N
AU  - Walker,PA
AU  - Kjaer,S
AU  - Riddell,A
AU  - Moore,C
AU  - Jebson,BR
AU  - Wilkinson,M
AU  - Marshall,LR
AU  - Rosser,EC
AU  - Radziszewska,A
AU  - Peckham,H
AU  - Ciurtin,C
AU  - Wedderburn,LR
AU  - Beale,R
AU  - Swanton,C
AU  - Gandhi,S
AU  - Stockinger,B
AU  - McCauley,J
AU  - Gambill,SJ
AU  - McCoy,LE
AU  - Cherepanov,P
AU  - Nastouli,E
AU  - Kassiotis,G
DO  - 10.1126/science.abe1107
EP  - 1343
PY  - 2020///
SN  - 0036-8075
SP  - 1339
TI  - Preexisting and de novo humoral immunity to SARS-CoV-2 in humans
T2  - Science
UR  - http://dx.doi.org/10.1126/science.abe1107
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000597271300046&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/86269
VL  - 370
ER  -
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