Imperial College London
Alternate

ProfessorGeorgiosKassiotis

Faculty of Medicine, Department of Infectious Disease

Professor of Retrovirology
 
 
 
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Contact

 

g.kassiotis Website

 
 
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Location

 

Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Fernandes:2022:10.1126/sciadv.abp8085,
author = {Fernandes, LP and Enriquez-Gasca, R and Gould, PA and Holt, JH and Conde, L and Ecco, G and Herrero, J and Gifford, R and Trono, D and Kassiotis, G and Rowe, HM},
doi = {10.1126/sciadv.abp8085},
journal = {Sci Adv},
title = {A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819.},
url = {http://dx.doi.org/10.1126/sciadv.abp8085},
volume = {8},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Mammalian genomes are a battleground for genetic conflict between repetitive elements and KRAB-zinc finger proteins (KZFPs). We asked whether KZFPs can regulate cell fate by using ZFP819, which targets a satellite DNA array, ZP3AR. ZP3AR coats megabase regions of chromosome 7 encompassing genes encoding ZSCAN4, a master transcription factor of totipotency. Depleting ZFP819 in mouse embryonic stem cells (mESCs) causes them to transition to a 2-cell (2C)-like state, whereby the ZP3AR array switches from a poised to an active enhancer state. This is accompanied by a global erosion of heterochromatin roadblocks, which we link to decreased SETDB1 stability. These events result in transcription of active LINE-1 elements and impaired differentiation. In summary, ZFP819 and TRIM28 partner up to close chromatin across Zscan4, to promote exit from totipotency. We propose that satellite DNAs may control developmental fate transitions by barcoding and switching off master transcription factor genes.
AU  - Fernandes,LP
AU  - Enriquez-Gasca,R
AU  - Gould,PA
AU  - Holt,JH
AU  - Conde,L
AU  - Ecco,G
AU  - Herrero,J
AU  - Gifford,R
AU  - Trono,D
AU  - Kassiotis,G
AU  - Rowe,HM
DO  - 10.1126/sciadv.abp8085
PY  - 2022///
TI  - A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819.
T2  - Sci Adv
UR  - http://dx.doi.org/10.1126/sciadv.abp8085
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36306355
VL  - 8
ER  -
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