Imperial College London
Alternate

DrGeraldLarrouy-Maumus

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lobato-Marquez:2018:10.1002/cm.21453,
author = {Lobato-Marquez, D and Krokowski, S and Sirianni, A and Larrouy-Maumus, G and Mostowy, S},
doi = {10.1002/cm.21453},
journal = {Cytoskeleton},
pages = {163--172},
title = {A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella},
url = {http://dx.doi.org/10.1002/cm.21453},
volume = {76},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Shigella flexneri, a Gramnegative enteroinvasive pathogen, causes inflammatory destruction of the human intestinal epithelium. During infection of epithelial cells, Shigella escape from the phagosome to the cytosol, where they reroute host cell glycolysis to obtain nutrients for proliferation. Septins, a poorly understood component of the cytoskeleton, can entrap cytosolic Shigella targeted to autophagy in cagelike structures to restrict bacterial proliferation. Although bacterial entrapment by septin caging has been the subject of intense investigation, the role of septins and the autophagy machinery in the proliferation of noncaged Shigella is mostly unknown. Here, we found that intracellular Shigella fail to efficiently proliferate in SEPT2, SEPT7 or p62/SQSTM1depleted cells. Consistent with a failure to proliferate, single cell analysis of bacteria not entrapped in septin cages showed that the number of metabolically active Shigella in septin or p62depleted cells is reduced. Targeted metabolomic analysis revealed that host cell glycolysis is dysregulated in septindepleted cells, suggesting a key role for septins in modulation of glycolysis. Together, these results suggest that septins and the autophagy machinery may regulate metabolic pathways that promote the proliferation intracellular Shigella not entrapped in septin cages.
AU  - Lobato-Marquez,D
AU  - Krokowski,S
AU  - Sirianni,A
AU  - Larrouy-Maumus,G
AU  - Mostowy,S
DO  - 10.1002/cm.21453
EP  - 172
PY  - 2018///
SN  - 1949-3584
SP  - 163
TI  - A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella
T2  - Cytoskeleton
UR  - http://dx.doi.org/10.1002/cm.21453
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/cm.21453
UR  - http://hdl.handle.net/10044/1/60046
VL  - 76
ER  -
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