Imperial College London
Alternate

DrGeraldLarrouy-Maumus

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dortet:2018:jac/dky330,
author = {Dortet, L and Bonnin, RA and Pennisi, I and Gauthier, L and Jousset, AB and Dabos, L and Furniss, RCD and Mavridou, DAI and Bogaerts, P and Glupczynski, Y and Potron, A and Plesiat, P and Beyrouthy, R and Robin, F and Bonnet, R and Naas, T and Filloux, A and Larrouy-Maumus, G},
doi = {jac/dky330},
journal = {Journal of Antimicrobial Chemotherapy},
pages = {3359--3367},
title = {Rapid detection and discrimination of chromosome-and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test},
url = {http://dx.doi.org/10.1093/jac/dky330},
volume = {73},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundPolymyxins are currently considered a last-resort treatment for infections caused by MDR Gram-negative bacteria. Recently, the emergence of carbapenemase-producing Enterobacteriaceae has accelerated the use of polymyxins in the clinic, resulting in an increase in polymyxin-resistant bacteria. Polymyxin resistance arises through modification of lipid A, such as the addition of phosphoethanolamine (pETN). The underlying mechanisms involve numerous chromosome-encoded genes or, more worryingly, a plasmid-encoded pETN transferase named MCR. Currently, detection of polymyxin resistance is difficult and time consuming.ObjectivesTo develop a rapid diagnostic test that can identify polymyxin resistance and at the same time differentiate between chromosome- and plasmid-encoded resistances.MethodsWe developed a MALDI-TOF MS-based method, named the MALDIxin test, which allows the detection of polymyxin resistance-related modifications to lipid A (i.e. pETN addition), on intact bacteria, in <15 min.ResultsUsing a characterized collection of polymyxin-susceptible and -resistant Escherichia coli, we demonstrated that our method is able to identify polymyxin-resistant isolates in 15 min whilst simultaneously discriminating between chromosome- and plasmid-encoded resistance. We validated the MALDIxin test on different media, using fresh and aged colonies and show that it successfully detects all MCR-1 producers in a blindly analysed set of carbapenemase-producing E. coli strains.ConclusionsThe MALDIxin test is an accurate, rapid, cost-effective and scalable method that represents a major advance in the diagnosis of polymyxin resistance by directly assessing lipid A modifications in intact bacteria.
AU  - Dortet,L
AU  - Bonnin,RA
AU  - Pennisi,I
AU  - Gauthier,L
AU  - Jousset,AB
AU  - Dabos,L
AU  - Furniss,RCD
AU  - Mavridou,DAI
AU  - Bogaerts,P
AU  - Glupczynski,Y
AU  - Potron,A
AU  - Plesiat,P
AU  - Beyrouthy,R
AU  - Robin,F
AU  - Bonnet,R
AU  - Naas,T
AU  - Filloux,A
AU  - Larrouy-Maumus,G
DO  - jac/dky330
EP  - 3367
PY  - 2018///
SN  - 0305-7453
SP  - 3359
TI  - Rapid detection and discrimination of chromosome-and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test
T2  - Journal of Antimicrobial Chemotherapy
UR  - http://dx.doi.org/10.1093/jac/dky330
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000452916600019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
VL  - 73
ER  -
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