Imperial College London
Alternate

DrGaryMcLean

Faculty of Medicine, National Heart & Lung Institute

Honorary Senior Research Fellow
 
 
 
//

Contact

 

+44 (0)20 7594 3775g.mclean

 
 
//

Location

 

Medical SchoolSt Mary's Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Narean:2019:10.1016/j.vaccine.2019.04.018,
author = {Narean, JS and Glanville, N and Nunn, CM and Niespodziana, K and Valenta, R and Johnston, SL and McLean, GR},
doi = {10.1016/j.vaccine.2019.04.018},
journal = {Vaccine},
pages = {2805--2813},
title = {Epitope mapping of antibodies induced with a conserved rhinovirus protein generating protective anti-rhinovirus immunity},
url = {http://dx.doi.org/10.1016/j.vaccine.2019.04.018},
volume = {37},
year = {2019}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Human rhinovirus (RV) infections are the principle cause of common colds and precipitate asthma and chronic obstructive pulmonary disease (COPD) exacerbations. Currently there is no vaccine for RV which is largely due to the existence of ∼160 serotypes/strains. We demonstrated previously that immunising mice with highly conserved VP4 and VP2 regions of the RV polyprotein (RV-A16 VP0) generated cross-reactive immunity to RV in vivo. The current study investigated and mapped the epitopes of RV-A16 VP0 that are targets for antibodies in serum samples from VP0 immunisation and RV challenge studies in mice. Recombinant capsid proteins, peptide pools and individual peptides spanning the immunogen sequence (RV-A16 VP0) were assessed for IgG binding sites to identify epitopes. We found that peptide pools covering the C-terminus of VP4, the N-terminus of VP2 and the neutralising NIm-II site within VP2 were bound by serum IgG from immunised mice. The NIm-II site peptide pool blocked IgG binding to the immunogen RV-A16 VP0 and individual peptides within the pool binding IgG were further mapped. Thus, we have identified immunodominant epitopes of RV vaccine candidate RV-A16 VP0, noting that strong IgG binding antibodies were observed that target a key neutralising epitope that is highly variable amongst RV serotypes.
AU  - Narean,JS
AU  - Glanville,N
AU  - Nunn,CM
AU  - Niespodziana,K
AU  - Valenta,R
AU  - Johnston,SL
AU  - McLean,GR
DO  - 10.1016/j.vaccine.2019.04.018
EP  - 2813
PY  - 2019///
SN  - 0264-410X
SP  - 2805
TI  - Epitope mapping of antibodies induced with a conserved rhinovirus protein generating protective anti-rhinovirus immunity
T2  - Vaccine
UR  - http://dx.doi.org/10.1016/j.vaccine.2019.04.018
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000468710400006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/71661
VL  - 37
ER  -
Download