Imperial College London
Alternate

Professor Graham P Taylor

Faculty of Medicine, Department of Infectious Disease

Professor of Human Retrovirology
 
 
 
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Contact

 

+44 (0)20 7594 3910g.p.taylor Website

 
 
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Location

 

443Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rosadas:2023:10.1515/cclm-2022-0904,
author = {Rosadas, C and Taylor, GP},
doi = {10.1515/cclm-2022-0904},
journal = {Clinical Chemistry and Laboratory Medicine},
pages = {1230--1234},
title = {Pre-analytical long-term stability of neopterin and neurofilament light in stored cerebrospinal fluid samples},
url = {http://dx.doi.org/10.1515/cclm-2022-0904},
volume = {61},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ObjectivesThe aim of this study was to evaluate the impact of long-term sample storage on the concentrations of neopterin and neurofilament light (Nfl) in cerebrospinal fluid (CSF) samples. These are useful markers of neuroinflammation and neuronal damage and have been applied as biomarkers for several neurological diseases. However, different pre-analytical variables have potential to influence results.MethodsTwenty-one CSF samples donated by patients with HTLV-1-associated myelopathy (HAM) and stored for up to 11 years at −80 °C were retested after three-years for neopterin (n=10) and Nfl (n=11) by ELISA.ResultsThere was a strong correlation between the paired results (r>0.98, p<0.0001). Neopterin concentrations (nmol/L) ranged from 12.4 to 64 initially and from 11.5 to 64.4 when retested, with means (SD) of 30 (18.4) 1st test and 33 (19.1) 2nd test. Nfl concentrations (pg/mL) ranged from 79.9 to 3,733 initially and from 86.3 to 3,332, when retested with means (SD) of 1,138 (1,272) 1st test and 1,009 (1,114) at re-test.ConclusionsStoring CSF samples at −80 °C appears not to impact the quantification of neopterin and Nfl allowing confidence in the reporting of archived samples.
AU  - Rosadas,C
AU  - Taylor,GP
DO  - 10.1515/cclm-2022-0904
EP  - 1234
PY  - 2023///
SN  - 1434-6621
SP  - 1230
TI  - Pre-analytical long-term stability of neopterin and neurofilament light in stored cerebrospinal fluid samples
T2  - Clinical Chemistry and Laboratory Medicine
UR  - http://dx.doi.org/10.1515/cclm-2022-0904
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000922163800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.degruyter.com/document/doi/10.1515/cclm-2022-0904/html
UR  - http://hdl.handle.net/10044/1/102745
VL  - 61
ER  -
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