Publications
673 results found
Tavare JM, Rutter GA, Griffiths MR, et al., 1996, Regulation of gene expression by insulin: Analysis in single living cells, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 24, Pages: 378-384, ISSN: 0300-5127
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- Citations: 4
Rutter GA, Burnett P, Brini M, et al., 1996, Imaging intramitochondrial Ca2+ in Chinese hamster ovary cells with recombinant target ed aequorin, JOURNAL OF PHYSIOLOGY-LONDON, Vol: 493P, Pages: P16-P17, ISSN: 0022-3751
Denton RM, McCormack JG, Rutter GA, et al., 1996, The hormonal regulation of pyruvate dehydrogenase complex, ADVANCES IN ENZYME REGULATION, VOL 36, Vol: 36, Pages: 183-198, ISSN: 0065-2571
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- Citations: 52
RUTTER GA, WHITE MRH, TAVARE JM, 1995, INVOLVEMENT OF MAP KINASE IN INSULIN SIGNALING REVEALED BY NONINVASIVE IMAGING OF LUCIFERASE GENE-EXPRESSION IN SINGLE LIVING CELLS (VOL 5, PG 890, 1995), CURRENT BIOLOGY, Vol: 5, Pages: 1072-1072, ISSN: 0960-9822
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- Citations: 2
RUTTER GA, WHITE MRH, TAVARE JM, 1995, INVOLVEMENT OF MAP KINASE IN INSULIN SIGNALING REVEALED BY NONINVASIVE IMAGING OF LUCIFERASE GENE-EXPRESSION IN SINGLE LIVING CELLS, CURRENT BIOLOGY, Vol: 5, Pages: 890-899, ISSN: 0960-9822
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- Citations: 67
RUTTER GA, GIROTTI M, SOOD T, et al., 1994, MAP KINASE IS ESSENTIAL FOR SIGNALING FROM THE INSULIN-RECEPTOR TO THE NUCLEUS, DIABETOLOGIA, Vol: 37, Pages: A31-A31, ISSN: 0012-186X
RUTTER GA, THELER JM, LI G, et al., 1994, CA2+ STORES IN INSULIN-SECRETING CELLS - LACK OF EFFECT OF CADP RIBOSE, CELL CALCIUM, Vol: 16, Pages: 71-80, ISSN: 0143-4160
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- Citations: 62
SEKINE N, CIRULLI V, REGAZZI R, et al., 1994, LOW LACTATE-DEHYDROGENASE AND HIGH MITOCHONDRIAL GLYCEROL PHOSPHATE DEHYDROGENASE IN PANCREATIC BETA-CELLS - POTENTIAL ROLE IN NUTRIENT SENSING, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 269, Pages: 4895-4902, ISSN: 0021-9258
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- Citations: 375
Rutter GA, Theler JM, Li G, et al., 1994, Ca<sup>2+</sup> stores in insulin-secreting cells: lack of effect of cADP ribose, Cell Calcium, Vol: 16, Pages: 71-80, ISSN: 0143-4160
Ca2+ stores were examined in several insulin secreting cell types by measuring uptake and release of Ca2+ by permeabilised cells. In pancreatic islet cells or INS-1 cells, <20% of the ATP-dependent, thapsigargin-sensitive Ca2+ pool could be released by saturating concentrations of inositol (1,4,5)P3 (InsP3). InsP3 released > 60% of the thapsigargin-sensitive Ca2+ pool in RINm5F cells. The total Ca2+ content of the thapsigargin-sensitive pool was similar in each of these cell types. Neither cADP ribose (cADPR; 1 μM) nor caffeine (10 mM) caused significant Ca2+ release from any of the permeabilised insulin-secreting cell preparations. ATP elicited similar increases in intracellular Ca2+ concentration ([Ca2+]i) in single, living INS-1 and RINm5F cells, and similar fold increases in InsP3 levels in cell populations. The Ca2+ ATPase inhibitor thapsigargin, added after ATP, caused smaller [Ca2+]i increases in RINm5F than in INS-1 cells. This is consistent with the presence of a smaller InsP3-sensitive Ca2+ pool in living INS-1 cells. The data indicate that InsP3 receptors are present in only a small subfraction of the Ca2+ ATPase-containing Ca2+ stores in INS-1 and pancreatic β-cells, and that cADP ribose/caffeine-sensitive Ca2+-induced Ca2+ release channels may be entirely absent from this endocrine cell type,. © 1994.
RUTTER GA, THELER JM, MURGIA M, et al., 1993, STIMULATED CA2+ INFLUX RAISES MITOCHONDRIAL FREE CA2+ TO SUPRAMICROMOLAR LEVELS IN A PANCREATIC BETA-CELL LINE - POSSIBLE ROLE IN GLUCOSE AND AGONIST-INDUCED INSULIN-SECRETION, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 268, Pages: 22385-22390, ISSN: 0021-9258
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- Citations: 174
SEKINE N, RUTTER GA, PRALONG WF, et al., 1993, EVALUATION OF NUTRIENT METABOLISM BY THE MTT TEST AND ITS CORRELATION TO INSULIN RELEASE IN INS-1 CELLS, DIABETOLOGIA, Vol: 36, Pages: A75-A75, ISSN: 0012-186X
LECLERC I, DAVIGNON I, LOPEZ D, et al., 1993, NO CHANGE IN GLUCOSE-TOLERANCE AND SUBSTRATE OXIDATION AFTER A HIGH-CARBOHYDRATE, LOW-FAT DIET, METABOLISM-CLINICAL AND EXPERIMENTAL, Vol: 42, Pages: 365-370, ISSN: 0026-0495
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- Citations: 11
RUTTER GA, PRALONG WF, WOLLHEIM CB, 1992, REGULATION OF MITOCHONDRIAL GLYCEROL-PHOSPHATE DEHYDROGENASE BY CA-2+ WITHIN ELECTROPERMEABILIZED INSULIN-SECRETING CELLS (INS-1), BIOCHIMICA ET BIOPHYSICA ACTA, Vol: 1175, Pages: 107-113, ISSN: 0006-3002
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- Citations: 74
RUTTER GA, LI G, PRALONG WF, et al., 1992, ALTERED SECRETORY RESPONSES OF INS-1 AND RINM5F CELLS ARE ASSOCIATED WITH DIFFERENCES IN INSP3 RECEPTOR FUNCTION, DIABETOLOGIA, Vol: 35, Pages: A99-A99, ISSN: 0012-186X
Rutter GA, Diggle TA, Denton RM, 1992, Regulation of pyruvate dehydrogenase by insulin and polyamines within electropermeabilized fat-cells and isolated mitochondria., Biochem J, Vol: 285 ( Pt 2), Pages: 435-439, ISSN: 0264-6021
1. Regulation of the mammalian pyruvate dehydrogenase (PDH) complex by insulin and polyamines has been examined by using electropermeabilized rat epididymal fat-cells and isolated mitochondria. The complex could be regulated within the permeabilized cells not only by insulin, but also by certain low-M(r) species, including Ca2+ and the polyamine spermidine. 2. Both spermine and spermidine increased the level of active dephosphorylated PDH (PDHa) in isolated adipose-tissue mitochondria 2-3-fold, with half-maximal effects at 0.9 mM and 1.7 mM respectively. By contrast, PDH activity in rat heart mitochondria was essentially insensitive to the effects of these polyamines. 3. The effects on PDH activity of incubation of adipose-tissue mitochondria with spermine persisted through re-isolation and re-incubation of the mitochondria in the absence of the polyamine. 4. No evidence was found of any increase in the concentration of spermine associated with purified mitochondrial fractions prepared from insulin-treated tissue. 5. Overall, the data provide further evidence against a role for polyamines in the rapid stimulation of PDH by insulin, but suggest that polyamines may be important in mediating longer-term changes in the activity of the complex.
RUTTER GA, DIGGLE TA, DENTON RM, 1992, REGULATION OF PYRUVATE-DEHYDROGENASE BY INSULIN AND POLYAMINES WITHIN ELECTROPERMEABILIZED FAT-CELLS AND ISOLATED-MITOCHONDRIA, BIOCHEMICAL JOURNAL, Vol: 285, Pages: 435-439, ISSN: 0264-6021
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- Citations: 28
MCCORMACK JG, DANIEL RL, OSBALDESTON NJ, et al., 1992, MITOCHONDRIAL CA2+ TRANSPORT AND THE ROLE OF MATRIX CA2+ IN MAMMALIAN-TISSUES, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 20, Pages: 153-159, ISSN: 0300-5127
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- Citations: 23
Rutter GA, Denton RM, 1992, Effects of insulin and guanosine 5'-[gamma-thio]triphosphate on fatty acid synthesis and lipolysis within electropermeabilized fat-cells., Biochem J, Vol: 281 ( Pt 2), Pages: 431-435, ISSN: 0264-6021
1. Exposure to electric fields ('electroporation') has been used to investigate the mechanism of the action of insulin on the regulation of fatty acid synthesis and lipolysis in isolated rat fat-cells. 2. Exposure of the cells to electric fields (six pulses at 2 or 3 kV/cm) permitted the uptake of a number of low-Mr molecules normally excluded from cells, including sucrose (Mr 342), EDTA (Mr 394) and propidium iodide (Mr 668). At least 90% of the cells were found to be permeable to these species. 3. Insulin stimulated the synthesis of fatty acids in electroporated (2 kV/cm) cells to a similar extent (0.68 +/- 0.19 to 6.7 +/- 0.7 micrograms-atoms of glucose carbon/h per g, or 3.3 +/- 0.6 to 11.2 +/- 1.6 micrograms-atoms of H2O hydrogen/h per g) to that observed in non-electroporated cells. Guanosine 5'-[gamma-thio]triphosphate (GTP[S]; 0.5 mM) also stimulated fatty acid synthesis (2-fold) and flux of glucose carbon into triacylglycerol glycerol (3-fold) in these cells, but had no effect on these parameters in non-electroporated cells. 4. Lipolysis in the electroporated cells was stimulated by isoprenaline and also by GTP[S], but only the effects of isoprenaline were inhibited by insulin. 5. Exposure to a higher field strength (3 kV/cm) gave results qualitatively similar to those described above, although the effects of insulin and isoprenaline were diminished. 6. These studies provide evidence against a role for changes in Ca2+, and probably also Na+, K+ or Mg2+, in insulin action on fat-cells, but may support a role for GTP-binding proteins.
RUTTER GA, DENTON RM, 1992, EFFECTS OF INSULIN AND GUANOSINE 5'-[GAMMA-THIO]TRIPHOSPHATE ON FATTY-ACID SYNTHESIS AND LIPOLYSIS WITHIN ELECTROPERMEABILIZED FAT-CELLS, BIOCHEMICAL JOURNAL, Vol: 281, Pages: 431-435, ISSN: 0264-6021
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- Citations: 18
RUTTER GA, BORTHWICK AC, DENTON RM, 1991, EFFECTS OF PROTEIN PHOSPHATASE INHIBITORS ON THE REGULATION OF INSULIN-SENSITIVE ENZYMES WITHIN RAT EPIDIDYMAL FAT-PADS AND CELLS, BIOCHEMICAL JOURNAL, Vol: 276, Pages: 649-654, ISSN: 0264-6021
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- Citations: 17
RUTTER GA, LEAKE MJ, MCCORMACK JG, et al., 1991, ROLE OF CA2+ AND MG2+ IN THE CONTROL OF THE PYRUVATE AND 2-OXOGLUTARATE DEHYDROGENASE COMPLEXES, INTERNATIONAL MEETING ON THE FUNCTION OF THIAMIN DIPHOSPHATE ENZYMES, Publisher: V C H PUBLISHERS, Pages: 221-231
RUTTER GA, OSBALDESTON NJ, MCCORMACK JG, et al., 1990, MEASUREMENT OF MATRIX FREE MG-2+ CONCENTRATION IN RAT-HEART MITOCHONDRIA BY USING ENTRAPPED FLUORESCENT-PROBES, BIOCHEMICAL JOURNAL, Vol: 271, Pages: 627-634, ISSN: 0264-6021
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- Citations: 65
RUTTER GA, OSBALDESTON NJ, MCCORMACK JG, et al., 1990, MEASUREMENT OF MATRIX MG-2+ CONCENTRATION OF RAT-HEART MITOCHONDRIA USING FLUORESCENT-PROBES, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 18, Pages: 894-895, ISSN: 0300-5127
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- Citations: 1
Rutter GA, 1990, Ca<sup>2+</sup> binding to citrate cycle dehydrogenases, International Journal of Biochemistry, Vol: 22, Pages: 1081-1088, ISSN: 0020-711X
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- Citations: 41
RUTTER GA, 1990, CA-2+-BINDING TO CITRATE CYCLE DEHYDROGENASES, INTERNATIONAL JOURNAL OF BIOCHEMISTRY, Vol: 22, Pages: 1081-1088, ISSN: 0020-711X
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- Citations: 45
RUTTER GA, MCCORMACK JG, MIDGLEY PJW, et al., 1989, THE ROLE OF CA-2+ IN THE HORMONAL-REGULATION OF THE ACTIVITIES OF PYRUVATE-DEHYDROGENASE AND OXOGLUTARATE DEHYDROGENASE COMPLEXES, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol: 573, Pages: 206-217, ISSN: 0077-8923
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- Citations: 14
DENTON RM, MIDGLEY PJW, RUTTER GA, et al., 1989, STUDIES INTO THE MECHANISM WHEREBY INSULIN ACTIVATES PYRUVATE-DEHYDROGENASE COMPLEX IN ADIPOSE-TISSUE, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol: 573, Pages: 285-296, ISSN: 0077-8923
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- Citations: 41
RUTTER GA, MIDGLEY PJW, DENTON RM, 1989, REGULATION OF THE PYRUVATE-DEHYDROGENASE COMPLEX BY CA-2+ WITHIN TOLUENE-PERMEABILIZED HEART-MITOCHONDRIA, BIOCHIMICA ET BIOPHYSICA ACTA, Vol: 1014, Pages: 263-270, ISSN: 0006-3002
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- Citations: 20
RUTTER GA, DENTON RM, 1989, RAPID PURIFICATION OF PIG-HEART NAD+-ISOCITRATE DEHYDROGENASE - STUDIES ON THE REGULATION OF ACTIVITY BY CA-2+, ADENINE-NUCLEOTIDES, MG-2+ AND OTHER METAL-IONS, BIOCHEMICAL JOURNAL, Vol: 263, Pages: 445-452, ISSN: 0264-6021
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- Citations: 19
RUTTER GA, DENTON RM, 1989, THE BINDING OF CA-2+ IONS TO PIG-HEART NAD+-ISOCITRATE DEHYDROGENASE AND THE 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, BIOCHEMICAL JOURNAL, Vol: 263, Pages: 453-462, ISSN: 0264-6021
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- Citations: 47
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