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@article{Sena:2011:10.1371/journal.pone.0021303,
author = {Sena, G and Frentz, Z and Birnbaum, KD and Leibler, S},
doi = {10.1371/journal.pone.0021303},
journal = {PLOS One},
pages = {e21303--e21303},
title = {Quantitation of cellular dynamics in growing Arabidopsis roots with light sheet microscopy.},
url = {http://dx.doi.org/10.1371/journal.pone.0021303},
volume = {6},
year = {2011}
}

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TY  - JOUR
AB  - To understand dynamic developmental processes, living tissues have to be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image, at cellular resolution, a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, to track cellular nuclei and to identify cell divisions. We demonstrate the system's capabilities by collecting data on divisions and nuclear dynamics.
AU  - Sena,G
AU  - Frentz,Z
AU  - Birnbaum,KD
AU  - Leibler,S
DO  - 10.1371/journal.pone.0021303
EP  - 21303
PY  - 2011///
SN  - 1932-6203
SP  - 21303
TI  - Quantitation of cellular dynamics in growing Arabidopsis roots with light sheet microscopy.
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0021303
UR  - http://hdl.handle.net/10044/1/21884
VL  - 6
ER  -

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