Imperial College London
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DrGiovanniSena

Faculty of Natural Sciences, Department of Life Sciences

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 7448g.sena Website

 
 
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Location

 

450Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Sena:2002,
author = {Sena, G and Nakajima, N and Jung, N and Benfey, P},
pages = {486--487},
title = {Analysis of Arabidopsis root pattern formation: Tissue-specific ectopic expression of the "Moving" putative transcription factor short-root},
year = {2002}
}
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RIS format (EndNote, RefMan)

TY  - CPAPER
AB  - The Arabidopsis root radial pattern is formed and maintained by well-defined asymmetric divisions of a set of stem cells (initials) ocated in the apical meristem. While it has been shown that this process is primarily dependent on positional information, little is known about the actual signaling mechanism. The short-root (shr) mutant is defective in one asymmetric division in the root meri- stem, so that the resulting radial pattern is missing one tissue (endodermis). The SHR gene, a putative transcription factor, in the root is expressed in the central tissue (stele), but not in the stem cells nor in the endodermis. We have shown (1) that the SHR protein appears to move from the stele into all the “nearest- neighbor” adjacent cells, where it is localized in the nuclei. No SHR is detectable in tissues more distant from the source stele. Nothing is known about the mechanism responsible for such nearest-neighbor movement. Moreover, it has also been shown (1) that ectopic expression of SHR can result in altered cell fates and multiplication of cell layers. Tissue-specific competence to regu- late SHR movement and to respond to SHR with alteration of cell fates and/or cell divisions seems to be part of the regulation of the positional information required for the establishment of the root radial pattern. Here we present preliminary data about ectopic expression, through tissue-specific promoters, of a fully functional protein fusion SHR-GFP. Its effect on radial pattern modification, cell fate alteration, and SHR-GFP movement will be discussed. 1. K. Nakajima et al., 2001, Nature 413, 307-311.
AU  - Sena,G
AU  - Nakajima,N
AU  - Jung,N
AU  - Benfey,P
EP  - 487
PY  - 2002///
SN  - 0012-1606
SP  - 486
TI  - Analysis of Arabidopsis root pattern formation: Tissue-specific ectopic expression of the "Moving" putative transcription factor short-root
ER  -
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