Imperial College London
Portrait Picture

Guy-Bart Stan

Faculty of Engineering, Department of Bioengineering

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 6375g.stan Website

 
 
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Location

 

B703Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Kylilis:2017:10.1101/231597,
author = {Kylilis, N and Stan, G-B and Polizzi, K},
doi = {10.1101/231597},
publisher = {bioRxiv},
title = {Tools for engineering coordinated system behaviour in synthetic microbial consortia},
url = {http://dx.doi.org/10.1101/231597},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - Advancing synthetic biology to the multicellular level requires the development of multiple orthogonal cell-to-cell communication channels to propagate information with minimal signal interference. The development of quorum sensing devices, the cornerstone technology for building microbial communities with coordinated system behaviour, has largely focused on reducing signal leakage between systems of cognate AHL/transcription factor pairs. However, the use of non-cognate signals as a design feature has received limited attention so far. Here, we demonstrate the largest library of AHL-receiver devices constructed to date with all cognate and non-cognate chemical signal interactions quantified and we develop a software tool that allows automated selection of orthogonal chemical channels. We use this approach to identify up to four orthogonal channels in silico and experimentally demonstrate the simultaneous use of three channels in co-culture. The development of multiple non-interfering cell-to-cell communication channels will facilitate the design of synthetic microbial consortia for novel applications including distributed bio-computation, increased bioprocess efficiency, cell specialisation, and spatial organisation.
AU  - Kylilis,N
AU  - Stan,G-B
AU  - Polizzi,K
DO  - 10.1101/231597
PB  - bioRxiv
PY  - 2017///
TI  - Tools for engineering coordinated system behaviour in synthetic microbial consortia
UR  - http://dx.doi.org/10.1101/231597
UR  - https://www.biorxiv.org/content/early/2017/12/11/231597
UR  - http://hdl.handle.net/10044/1/63256
ER  -
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