Imperial College London
Emeritus Professor Herb Arst

Emeritus ProfessorHerbArst

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor of Microbial Genetics
 
 
 
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Contact

 

+44 (0)20 7594 2073h.arst

 
 
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Location

 

5.40Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hervás-Aguilar:2010:10.1016/j.fgb.2010.03.010,
author = {Hervás-Aguilar, A and Rodríguez-Galán, O and Galindo, A and Abenza, JF and Arst, HN and Peñalva, MA},
doi = {10.1016/j.fgb.2010.03.010},
journal = {Fungal Genet Biol},
pages = {636--646},
title = {Characterization of Aspergillus nidulans DidB Did2, a non-essential component of the multivesicular body pathway.},
url = {http://dx.doi.org/10.1016/j.fgb.2010.03.010},
volume = {47},
year = {2010}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ESCRT-III heteropolymers mediate membrane protein cargo sorting into multivesicular endosomes for subsequent vacuolar degradation. We studied the localization of largely uncharacterized Aspergillus nidulans ESCRT-III using its key structural component Vps32 and the 'associated' component DidB(Did2). Vps32-GFP localizes to motile early endosomes as reported, but predominates in aggregates often associated with vacuoles due to inability to dissociate from endosomes. DidB(Did)(2) regulating Vps4 (the ATPase disassembling ESCRT-III) is not essential. Consistent with this accessory role, didB Delta is unable to block the MVB sorting of the glutamate transporter AgtA, but increases its steady-state level and mislocalizes a fraction of the permease to the plasma membrane under conditions promoting its vacuolar targeting. didB Delta exacerbates the dominant-negative growth defect resulting from Vps32-GFP over-expression. A proportion of DidB-GFP is detectable in early endosomes colocalizing with RabA(Rab5) and accumulating in nudA1 tips, suggesting that ESCRT-III assembles on endosomes from the early steps of the endocytic pathway.
AU  - Hervás-Aguilar,A
AU  - Rodríguez-Galán,O
AU  - Galindo,A
AU  - Abenza,JF
AU  - Arst,HN
AU  - Peñalva,MA
DO  - 10.1016/j.fgb.2010.03.010
EP  - 646
PY  - 2010///
SN  - 1087-1845
SP  - 636
TI  - Characterization of Aspergillus nidulans DidB Did2, a non-essential component of the multivesicular body pathway.
T2  - Fungal Genet Biol
UR  - http://dx.doi.org/10.1016/j.fgb.2010.03.010
VL  - 47
ER  -
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