Imperial College London
Emeritus Professor Herb Arst

Emeritus ProfessorHerbArst

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor of Microbial Genetics
 
 
 
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Contact

 

+44 (0)20 7594 2073h.arst

 
 
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Location

 

5.40Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lucena-Agell:2015:10.1128/EC.00031-15,
author = {Lucena-Agell, D and Galindo, A and Arst, HN and Penalva, MA},
doi = {10.1128/EC.00031-15},
journal = {Eukaryotic Cell},
pages = {545--553},
title = {Aspergillus nidulans ambient pH signaling does not require endocytosis},
url = {http://dx.doi.org/10.1128/EC.00031-15},
volume = {14},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Aspergillus nidulans (Pal) ambient pH signaling takes place in cortical structures containing components of the ESCRT pathway, which are hijacked by the alkaline pH-activated, ubiquitin-modified version of the arrestin-like protein PalF and taken to the plasma membrane. There, ESCRTs scaffold the assembly of dedicated Pal proteins acting downstream. The molecular details of this pathway, which results in the two-step proteolytic processing of the transcription factor PacC, have received considerable attention due to the key role that it plays in fungal pathogenicity. While current evidence strongly indicates that the pH signaling role of ESCRT complexes is limited to plasma membrane-associated structures where PacC proteolysis would take place, the localization of the PalB protease, which almost certainly catalyzes the first and only pH-regulated proteolytic step, had not been investigated. In view of ESCRT participation, this formally leaves open the possibility that PalB activation requires endocytic internalization. As endocytosis is essential for hyphal growth, nonlethal endocytic mutations are predicted to cause an incomplete block. We used a SynA internalization assay to measure the extent to which any given mutation prevents endocytosis. We show that none of the tested mutations impairing endocytosis to different degrees, including slaB1, conditionally causing a complete block, have any effect on the activation of the pathway. We further show that PalB, like PalA and PalC, localizes to cortical structures in an alkaline pH-dependent manner. Therefore, signaling through the Pal pathway does not involve endocytosis.
AU  - Lucena-Agell,D
AU  - Galindo,A
AU  - Arst,HN
AU  - Penalva,MA
DO  - 10.1128/EC.00031-15
EP  - 553
PY  - 2015///
SN  - 1535-9778
SP  - 545
TI  - Aspergillus nidulans ambient pH signaling does not require endocytosis
T2  - Eukaryotic Cell
UR  - http://dx.doi.org/10.1128/EC.00031-15
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000355543000003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/33695
VL  - 14
ER  -
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