Imperial College London
Emeritus Professor Herb Arst

Emeritus ProfessorHerbArst

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor of Microbial Genetics
 
 
 
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Contact

 

+44 (0)20 7594 2073h.arst

 
 
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Location

 

5.40Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Arst:2015:10.1111/mmi.13173,
author = {Arst, HN and Bussink, H-J and Bignell, EM and Munera, Huertas T and Lucena-Agell, D and Scazzocchio, C and Espeso, EA and Bertuzzi, M and Rudnicka, J and Negrete-Urtasun, S and Penas-Parilla, MM and Rainbow, L and Penalva, MA and Tilburn, J},
doi = {10.1111/mmi.13173},
journal = {Molecular Microbiology},
pages = {1051--1072},
title = {Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein},
url = {http://dx.doi.org/10.1111/mmi.13173},
volume = {98},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The Aspergillus nidulans PacC transcription factor mediates gene regulation in response to alkaline ambient pH which, signalled by the Pal pathway, results in the processing of PacC² to PacC² via PacC³. Here we investigate two levels at which the pH regulatory system is transcriptionally moderated by pH and identify and characterise a new component of the pH regulatory machinery, PacX. Transcript level analysis and overexpression studies demonstrate that repression of acid-expressed palF, specifying the Pal pathway arrestin, probably by PacC² and/or PacC³, prevents an escalating alkaline pH response. Transcript analyses using a reporter and constitutively expressed pacCtrans-alleles show that pacC preferential alkaline-expression results from derepression by depletion of the acid-prevalent PacC² form. We additionally show that pacC repression requires PacX. pacX mutations suppress PacC processing recalcitrant mutations, in part, through derepressed PacC levels resulting in traces of PacC² formed by pH-independent proteolysis. pacX was cloned by impala transposon mutagenesis. PacX, with homologues within the Leotiomyceta, has an unusual structure with an amino-terminal coiled-coil and a carboxy-terminal zinc binuclear cluster. pacX mutations indicate the importance of these regions. One mutation, an unprecedented finding in A. nidulans genetics, resulted from an insertion of an endogenous Fot1-like transposon.
AU  - Arst,HN
AU  - Bussink,H-J
AU  - Bignell,EM
AU  - Munera,Huertas T
AU  - Lucena-Agell,D
AU  - Scazzocchio,C
AU  - Espeso,EA
AU  - Bertuzzi,M
AU  - Rudnicka,J
AU  - Negrete-Urtasun,S
AU  - Penas-Parilla,MM
AU  - Rainbow,L
AU  - Penalva,MA
AU  - Tilburn,J
DO  - 10.1111/mmi.13173
EP  - 1072
PY  - 2015///
SN  - 1365-2958
SP  - 1051
TI  - Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/mmi.13173
UR  - http://hdl.handle.net/10044/1/27475
VL  - 98
ER  -
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