Imperial College London
Emeritus Professor Herb Arst

Emeritus ProfessorHerbArst

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor of Microbial Genetics
 
 
 
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Contact

 

+44 (0)20 7594 2073h.arst

 
 
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Location

 

5.40Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{López-Berges:2017:femsle/fnx065,
author = {López-Berges, MS and Arst, HN and Pinar, M and Peñalva, MA},
doi = {femsle/fnx065},
journal = {FEMS Microbiology Letters},
title = {Genetic studies on the physiological role of CORVET in Aspergillus nidulans.},
url = {http://dx.doi.org/10.1093/femsle/fnx065},
volume = {364},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - CORVET and HOPS are protein complexes mediating the maturation of early endosomes (EEs) into late endosomes (LEs)/vacuoles. These hetero-hexamers share four 'core' components, Vps11, Vps16, Vps18 and Vps33, and differ in two specific subunits, CORVET Vps8 and Vps3 and HOPS Vps39 and Vps41. Whereas ablating HOPS-specific components has minor growth effects, ablating any CORVET constituent severely debilitates Aspergillus nidulans growth, buttressing previous work indicating that maturation of EEs into LEs is physiologically crucial. A genetic screen revealed that impairing the slt cation homeostasis pathway rescues the growth defect resulting from inactivation of the 'core' protein Vps33. Subsequent genetic analyses showed that the defect resulting from lack of any one of the five other CORVET components could similarly be rescued by sltAΔ eliminating the slt regulator SltA. Whereas double deletants lacking functionally non-equivalent components of the CORVET and HOPS complexes are rescued by sltAΔ, those lacking functionally equivalent components are not, suggesting that intermediate 'hybrid' complexes previously detected in yeast are physiologically relevant. vps3Δ, vps8Δ, vps39Δ and vps41Δ result in small vacuoles. This phenotype is remediable by sltAΔ in the case of CORVET-specific, but not in the case of HOPS-specific deletants, indicating that the slt- effect on vacuolar size necessitates HOPS.
AU  - López-Berges,MS
AU  - Arst,HN
AU  - Pinar,M
AU  - Peñalva,MA
DO  - femsle/fnx065
PY  - 2017///
SN  - 0378-1097
TI  - Genetic studies on the physiological role of CORVET in Aspergillus nidulans.
T2  - FEMS Microbiology Letters
UR  - http://dx.doi.org/10.1093/femsle/fnx065
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28379362
UR  - http://hdl.handle.net/10044/1/46187
VL  - 364
ER  -
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