Imperial College London
Emeritus Professor Herb Arst

Emeritus ProfessorHerbArst

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor of Microbial Genetics
 
 
 
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Contact

 

+44 (0)20 7594 2073h.arst

 
 
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Location

 

5.40Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hernandez-Gonzalez:2018:10.1371/journal.pgen.1007291,
author = {Hernandez-Gonzalez, M and Bravo-Plaza, I and Pinar, M and de, los Rios V and Arst, HN and Penalva, MA},
doi = {10.1371/journal.pgen.1007291},
journal = {PLoS Genetics},
title = {Endocytic recycling via the TGN underlies the polarized hyphal mode of growth},
url = {http://dx.doi.org/10.1371/journal.pgen.1007291},
volume = {14},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Intracellular traffic in Aspergillus nidulans hyphae must cope with the challenges that the high rates of apical extension (1μm/min) and the long intracellular distances (>100 μm) impose. Understanding the ways in which the hyphal tip cell coordinates traffic to meet these challenges is of basic importance, but is also of considerable applied interest, as fungal invasiveness of animals and plants depends critically upon maintaining these high rates of growth. Rapid apical extension requires localization of cell-wall-modifying enzymes to hyphal tips. By combining genetic blocks in different trafficking steps with multidimensional epifluorescence microscopy and quantitative image analyses we demonstrate that polarization of the essential chitin-synthase ChsB occurs by indirect endocytic recycling, involving delivery/exocytosis to apices followed by internalization by the sub-apical endocytic collar of actin patches and subsequent trafficking to TGN cisternae, where it accumulates for ~1 min before being re-delivered to the apex by a RAB11/TRAPPII-dependent pathway. Accordingly, ChsB is stranded at the TGN by Sec7 inactivation but re-polarizes to the apical dome if the block is bypassed by a mutation in geaAgea1 that restores growth in the absence of Sec7. That polarization is independent of RAB5, that ChsB predominates at apex-proximal cisternae, and that upon dynein impairment ChsB is stalled at the tips in an aggregated endosome indicate that endocytosed ChsB traffics to the TGN via sorting endosomes functionally located upstream of the RAB5 domain and that this step requires dynein-mediated basipetal transport. It also requires RAB6 and its effector GARP (Vps51/Vps52/Vps53/Vps54), whose composition we determined by MS/MS following affinity chromatography purification. Ablation of any GARP component diverts ChsB to vacuoles and impairs growth and morphology markedly, emphasizing the important physiological role played by this pathway that, we propose, is ce
AU  - Hernandez-Gonzalez,M
AU  - Bravo-Plaza,I
AU  - Pinar,M
AU  - de,los Rios V
AU  - Arst,HN
AU  - Penalva,MA
DO  - 10.1371/journal.pgen.1007291
PY  - 2018///
SN  - 1553-7390
TI  - Endocytic recycling via the TGN underlies the polarized hyphal mode of growth
T2  - PLoS Genetics
UR  - http://dx.doi.org/10.1371/journal.pgen.1007291
UR  - http://hdl.handle.net/10044/1/58386
VL  - 14
ER  -
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