Imperial College London
Alternate

DrHutanAshrafian

Faculty of Medicine, Department of Surgery & Cancer

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 3312 7651h.ashrafian

 
 
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Location

 

1089Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wei:2022:2050-6120/ac9513,
author = {Wei, J and Monfort, Sanchez E and Avery, J and Barbouti, O and Hoare, J and Ashrafian, H and Darzi, A and Thompson, A},
doi = {2050-6120/ac9513},
journal = {Methods and Applications in Fluorescence},
pages = {1--9},
title = {Non-invasive assessment of intestinal permeability in healthy volunteers using transcutaneous fluorescence spectroscopy},
url = {http://dx.doi.org/10.1088/2050-6120/ac9513},
volume = {10},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The permeability of the intestinal barrier is altered in a multitude of gastrointestinal conditions such as Crohn's and coeliac disease. However, the clinical utility of gut permeability is currently limited due to a lack of reliable diagnostic tests. To address this issue, we report a novel technique for rapid, non-invasive measurement of gut permeability based on transcutaneous ('through-the-skin') fluorescence spectroscopy. In this approach, participants drink an oral dose of a fluorescent dye (fluorescein) and a fibre-optic fluorescence spectrometer is attached to the finger to detect permeation of the dye from the gut into the blood stream in a non-invasive manner. To validate this technique, clinical trial measurements were performed in 11 healthy participants. First, after 6 h of fasting, participants ingested 500 mg of fluorescein dissolved in 100 ml of water and fluorescence measurements were recorded at the fingertip over the following 3 h. All participants were invited back for a repeat study, this time ingesting the same solution but with 60 g of sugar added (known to transiently increase intestinal permeability). Results from the two study datasets (without and with sugar respectively) were analysed and compared using a number of analysis procedures. This included both manual and automated calculation of a series of parameters designed for assessment of gut permeability. Calculated values were compared using Student's T-tests, which demonstrated significant differences between the two datasets. Thus, transcutaneous fluorescence spectroscopy shows promise in non-invasively discriminating between two differing states of gut permeability, demonstrating potential for future clinical use.
AU  - Wei,J
AU  - Monfort,Sanchez E
AU  - Avery,J
AU  - Barbouti,O
AU  - Hoare,J
AU  - Ashrafian,H
AU  - Darzi,A
AU  - Thompson,A
DO  - 2050-6120/ac9513
EP  - 9
PY  - 2022///
SN  - 2050-6120
SP  - 1
TI  - Non-invasive assessment of intestinal permeability in healthy volunteers using transcutaneous fluorescence spectroscopy
T2  - Methods and Applications in Fluorescence
UR  - http://dx.doi.org/10.1088/2050-6120/ac9513
UR  - https://iopscience.iop.org/article/10.1088/2050-6120/ac9513/meta
UR  - http://hdl.handle.net/10044/1/104611
VL  - 10
ER  -
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