Imperial College London
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ProfessorHectorKeun

Faculty of Medicine, Department of Surgery & Cancer

Professor of Biochemistry
 
 
 
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Contact

 

+44 (0)20 7594 3161h.keun

 
 
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Location

 

officesInstitute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bravo-Santano:2018:10.1128/mSphere.00374-18,
author = {Bravo-Santano, N and Ellis, JK and Mateos, LM and Calle, Y and Keun, HC and Behrends, V and Letek, M},
doi = {10.1128/mSphere.00374-18},
journal = {MSPHERE},
title = {Intracellular Staphylococcus aureus Modulates Host Central Carbon Metabolism To Activate Autophagy},
url = {http://dx.doi.org/10.1128/mSphere.00374-18},
volume = {3},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Staphylococcus aureus is a facultative intracellular pathogen that invades and replicates within many types of phagocytic and nonphagocytic cells. During intracellular infection, S. aureus is capable of subverting xenophagy and escaping to the cytosol of the host cell. Furthermore, drug-induced autophagy facilitates the intracellular replication of S. aureus, but the reasons behind this are unclear. Here, we have studied the host central carbon metabolism during S. aureus intracellular infection. We found extensive metabolic rerouting and detected several distinct metabolic changes that suggested starvation-induced autophagic flux in infected cells. These changes included increased uptake but lower intracellular levels of glucose and low abundance of several essential amino acids, as well as markedly upregulated glutaminolysis. Furthermore, we show that AMP-activated protein kinase (AMPK) and extracellular signal-regulated kinase (ERK) phosphorylation levels are significantly increased in infected cells. Interestingly, while autophagy was activated in response to S. aureus invasion, most of the autophagosomes detected in infected cells did not contain bacteria, suggesting that S. aureus induces the autophagic flux during cell invasion for energy generation and nutrient scavenging. Accordingly, AMPK inhibition halted S. aureus intracellular proliferation.
AU  - Bravo-Santano,N
AU  - Ellis,JK
AU  - Mateos,LM
AU  - Calle,Y
AU  - Keun,HC
AU  - Behrends,V
AU  - Letek,M
DO  - 10.1128/mSphere.00374-18
PY  - 2018///
SN  - 2379-5042
TI  - Intracellular Staphylococcus aureus Modulates Host Central Carbon Metabolism To Activate Autophagy
T2  - MSPHERE
UR  - http://dx.doi.org/10.1128/mSphere.00374-18
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000441058200036&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/63005
VL  - 3
ER  -
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