Imperial College London
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ProfessorHectorKeun

Faculty of Medicine, Department of Surgery & Cancer

Professor of Biochemistry
 
 
 
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Contact

 

+44 (0)20 7594 3161h.keun

 
 
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Location

 

officesInstitute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Siskos:2016:10.1021/acs.analchem.6b02930,
author = {Siskos, AP and Jain, P and Romisch-Margl, W and Bennet, M and Achaintre, D and Asad, Y and Marney, L and Richardson, L and Koulman, A and Griffin, JL and Raynaud, F and Scalbert, A and Adamski, J and Prehn, C and Keun, HC},
doi = {10.1021/acs.analchem.6b02930},
journal = {Analytical Chemistry},
pages = {656--665},
title = {Interlaboratory reproducibility of a targeted metabolomics platform for analysis of human serum and plasma},
url = {http://dx.doi.org/10.1021/acs.analchem.6b02930},
volume = {89},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A critical question facing the field of metabolomics is whether data obtained from different centers can be effectively compared and combined. An important aspect of this is the interlaboratory precision (reproducibility) of the analytical protocols used. We analyzed human samples in six laboratories using different instrumentation but a common protocol (the AbsoluteIDQ p180 kit) for the measurement of 189 metabolites via liquid chromatography (LC) or flow injection analysis (FIA) coupled to tandem mass spectrometry (MS/MS). In spiked quality control (QC) samples 82% of metabolite measurements had an interlaboratory precision of <20%, while 83% of averaged individual laboratory measurements were accurate to within 20%. For 20 typical biological samples (serum and plasma from healthy individuals) the median interlaboratory coefficient of variation (CV) was 7.6%, with 85% of metabolites exhibiting a median interlaboratory CV of <20%. Precision was largely independent of the type of sample (serum or plasma) or the anticoagulant used but was reduced in a sample from a patient with dyslipidaemia. The median interlaboratory accuracy and precision of the assay for standard reference plasma (NIST SRM 1950) were 107% and 6.7%, respectively. Likely sources of irreproducibility were the near limit of detection (LOD) typical abundance of some metabolites and the degree of manual review and optimization of peak integration in the LC–MS/MS data after acquisition. Normalization to a reference material was crucial for the semi-quantitative FIA measurements. This is the first interlaboratory assessment of a widely used, targeted metabolomics assay illustrating the reproducibility of the protocol and how data generated on different instruments could be directly integrated in large-scale epidemiological studies.
AU  - Siskos,AP
AU  - Jain,P
AU  - Romisch-Margl,W
AU  - Bennet,M
AU  - Achaintre,D
AU  - Asad,Y
AU  - Marney,L
AU  - Richardson,L
AU  - Koulman,A
AU  - Griffin,JL
AU  - Raynaud,F
AU  - Scalbert,A
AU  - Adamski,J
AU  - Prehn,C
AU  - Keun,HC
DO  - 10.1021/acs.analchem.6b02930
EP  - 665
PY  - 2016///
SN  - 1086-4377
SP  - 656
TI  - Interlaboratory reproducibility of a targeted metabolomics platform for analysis of human serum and plasma
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.6b02930
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000391346600050&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/44699
VL  - 89
ER  -
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