Publications
495 results found
Garner OB, Aguilar HC, Fulcher JA, et al., 2010, Endothelial Galectin-1 Binds to Specific Glycans on Nipah Virus Fusion Protein and Inhibits Maturation, Mobility, and Function to Block Syncytia Formation, PLOS PATHOGENS, Vol: 6, ISSN: 1553-7366
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- Citations: 52
Takamatsu S, Antonopoulos A, Ohtsubo K, et al., 2010, Physiological and glycomic characterization of <i>N</i>-acetylglucosaminyltransferase-IVa and -IVb double deficient mice, GLYCOBIOLOGY, Vol: 20, Pages: 485-497, ISSN: 0959-6658
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- Citations: 46
Canis K, McKinnon TAJ, Nowak A, et al., 2010, The plasma von Willebrand factor <i>O</i>-glycome comprises a surprising variety of structures including ABH antigens and disialosyl motifs, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 8, Pages: 137-145, ISSN: 1538-7933
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- Citations: 58
Peyfoon E, Meyer B, Hitchen PG, et al., 2010, The <i>S</i>-Layer Glycoprotein of the Crenarchaeote <i>Sulfolobus acidocaldarius</i> Is Glycosylated at Multiple Sites with Chitobiose-Linked <i>N</i>-Glycans, ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL, Vol: 2010, ISSN: 1472-3646
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- Citations: 64
Rogers DW, Baldini F, Battaglia F, et al., 2009, Transglutaminase-Mediated Semen Coagulation Controls Sperm Storage in the Malaria Mosquito, PLOS BIOLOGY, Vol: 7, ISSN: 1545-7885
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- Citations: 96
Pang P, Tissot B, Drobnis EZ, et al., 2009, Analysis of the Human Seminal Plasma Glycome Reveals the Presence of Immunomodulatory Carbohydrate Functional Groups, Journal of Proteome Research, Vol: 8, Pages: 4906-4915, ISSN: 1535-3893
Mitoma J, Miyazaki T, Sutton-Smith M, et al., 2009, The <i>N</i>-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells, GLYCOCONJUGATE JOURNAL, Vol: 26, Pages: 511-523, ISSN: 0282-0080
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- Citations: 24
Tissot B, North SJ, Ceroni A, et al., 2009, Challenges in quantitative proteomics and posttranslational protein modifications, 34th Congress of the Federation-of-European-Biochemical-Societies, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 89-89, ISSN: 1742-464X
Tissot B, North SJ, Ceroni A, et al., 2009, Glycoproteomics: Past, present and future, FEBS Letters, Vol: 583, Pages: 1728-1735, ISSN: 0014-5793
This invited paper reviews the study of protein glycosylation, commonly known as glycoproteomics, beginning with the origins of the subject area in the early 1970s shortly after mass spectrometry was first applied to protein sequencing. We go on to describe current analytical approaches to glycoproteomic analyses, with exemplar projects presented in the form of the complex story of human glycodelin and the characterisation of blood group H eptitopes on the O-glycans of gp273 from Unio elongatulus. Finally, we present an update on the latest progress in the field of automated and semi-automated interpretation and annotation of these data in the form of GlycoWorkBench, a powerful informatics tool that provides valuable assistance in unravelling the complexities of glycoproteomic studies.
Lee C, Pang P, Yeung WSB, et al., 2009, Effects of Differential Glycosylation of Glycodelins on Lymphocyte Survival, Journal of Biological Chemistry, Vol: 284, Pages: 15084-15096
Glycodelin is a human glycoprotein with four reported glycoforms, namely glycodelin-A (GdA), glycodelin-F (GdF), glycodelin-C (GdC), and glycodelin-S (GdS). These glycoforms have the same protein core and appear to differ in their N-glycosylation. The glycosylation of GdA is completely different from that of GdS. GdA inhibits proliferation and induces cell death of T cells. However, the glycosylation and immunomodulating activities of GdF and GdC are not known. This study aimed to use ultra-high sensitivity mass spectrometry to compare the glycomes of GdA, GdC, and GdF and to study the relationship between the immunological activity and glycosylation pattern among glycodelin glycoforms. Using MALDI-TOF strategies, the glycoforms were shown to contain an enormous diversity of bi-, tri-, and tetra-antennary complex-type glycans carrying Galβ1–4GlcNAc (lacNAc) and/or GalNAcβ1–4GlcNAc (lacdiNAc) antennae backbones with varying levels of fucose and sialic acid substitution. Interestingly, they all carried a family of Sda (NeuAcα2–3(GalNAcβ1–4)Gal)-containing glycans, which were not identified in the earlier study because of less sensitive methodologies used. Among the three glycodelins, GdA is the most heavily sialylated. Virtually all the sialic acid on GdC is located on the Sda antennae. With the exception of the Sda epitope, the GdC N-glycome appears to be the asialylated counterpart of the GdA/GdF glycomes. Sialidase activity, which may be responsible for transforming GdA/GdF to GdC, was detected in cumulus cells. Both GdA and GdF inhibited the proliferation, induced cell death, and suppressed interleukin-2 secretion of Jurkat cells and peripheral blood mononuclear cells. In contrast, no immunosuppressive effect was observed for GdS and GdC.
Pang PC, Tissot B, Drobnis EZ, et al., 2009, GLYCOMIC ANALYSIS OF HUMAN SEMINAL PLASMA REVEALS THE PRESENCE OF IMMUNOMODULATORY CARBOHYDRATE FUNCTIONAL GROUPS, 34th Annual Meeting of the American-Society-of-Andrology, Publisher: AMER SOC ANDROLOGY, INC, Pages: 54-55, ISSN: 0196-3635
Qazi O, Hitchen P, Tissot B, et al., 2009, Mass spectrometric analysis of the S-layer proteins from <i>Clostridium difficile</i> demonstrates the absence of glycosylation, JOURNAL OF MASS SPECTROMETRY, Vol: 44, Pages: 368-374, ISSN: 1076-5174
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- Citations: 16
Wehmeier S, Varghese AS, Gurcha SS, et al., 2009, Glycosylation of the phosphate binding protein, PstS, in <i>Streptomyces coelicolor</i> by a pathway that resembles protein O-mannosylation in eukaryotes, MOLECULAR MICROBIOLOGY, Vol: 71, Pages: 421-433, ISSN: 0950-382X
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- Citations: 44
Tissot B, Ceroni A, Powell AK, et al., 2008, Software Tool for the Structural Determination of Glycosaminoglycans by Mass Spectrometry, ANALYTICAL CHEMISTRY, Vol: 80, Pages: 9204-9212, ISSN: 0003-2700
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- Citations: 31
Huang L, Hollingsworth RI, Haslam SM, et al., 2008, The Lan3-2 glycoepitope of <i>Hirudo medicinalis</i> consists of β-(1,4)-linked mannopyranose Cell type-specific glycans of <i>H-medicinalis</i>, JOURNAL OF NEUROCHEMISTRY, Vol: 107, Pages: 1448-1456, ISSN: 0022-3042
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- Citations: 3
Yurist-Doutsch S, Abu-Qarn M, Battaglia F, et al., 2008, <i>aglF</i>, <i>aglG</i> and <i>aglI</i>, novel members of a gene island involved in the N-glycosylation of the <i>Haloferax volcanii</i> S-layer glycoprotein, MOLECULAR MICROBIOLOGY, Vol: 69, Pages: 1234-1245, ISSN: 0950-382X
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- Citations: 60
Mishra AK, Klein C, Gurcha SS, et al., 2008, Structural characterization and functional properties of a novel lipomannan variant isolated from a <i>Corynebacterium glutamicum pimB</i>′ mutant, ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, Vol: 94, Pages: 277-287, ISSN: 0003-6072
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- Citations: 27
Aleksic M, Pease CK, Basketter DA, et al., 2008, Mass spectrometric identification of covalent adducts of the skin allergen 2,4-dinitro-1-chlorobenzene and model skin proteins, TOXICOLOGY IN VITRO, Vol: 22, Pages: 1169-1176, ISSN: 0887-2333
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- Citations: 35
Sun W, Parry S, Panico M, et al., 2008, <i>N</i>-glycans and the N terminus of protein C inhibitor affect the cofactor-enhanced rates of thrombin inhibition, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 283, Pages: 18601-18611
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- Citations: 17
Shi W, Huang Y, Sutton-Smith M, et al., 2008, A filovirus-unique region of Ebola virus nucleoprotein confers aberrant migration and mediates its incorporation into virions, JOURNAL OF VIROLOGY, Vol: 82, Pages: 6190-6199, ISSN: 0022-538X
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- Citations: 31
Abu-Qarn M, Giordano A, Battaglia F, et al., 2008, Identification of AglE, a second glycosyltransferase involved in N glycosylation of the <i>Haloferax volcanii</i> S-layer glycoprotein, JOURNAL OF BACTERIOLOGY, Vol: 190, Pages: 3140-3146, ISSN: 0021-9193
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- Citations: 57
Abu-Qarn M, Yurist-Doutsch S, Giordano A, et al., 2007, <i>Haloferax volcanii</i> AglB and AglD are involved in N-glycosylation of the S-layer glycoprotein and proper assembly of the surface layer, JOURNAL OF MOLECULAR BIOLOGY, Vol: 374, Pages: 1224-1236, ISSN: 0022-2836
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- Citations: 112
Pang P, Tissot B, Drobnis EZ, et al., 2007, Expression of Bisecting Type and Lewisx/Lewisy Terminated N-Glycans on Human Sperm, Journal of Biological Chemistry, Vol: 282, Pages: 36593-36602
Human sperm lack major histocompatibility class I molecules, making them susceptible to lysis by natural killer (NK) cells. Major histocompatibility class I negative tumor cells block NK cell lysis by expressing sufficient amounts of bisecting type N-glycans on their surfaces. Therefore, sperm could employ the same strategy to evade NK cell lysis. The total N-glycans derived from sperm were sequenced using ultrasensitive mass spectrometric and conventional approaches. Three major classes of N-glycans were detected, (i) high mannose, (ii) biantennary bisecting type, and (iii) biantennary, triantennary, and tetraantennary oligosaccharides terminated with Lewisx and Lewisy sequences. Immunostaining of normal sperm showed that glycoproteins bearing Lewisy sequences are localized to the acrosome and not the plasma membrane. In contrast, defective sperm showed distinct surface labeling with anti-Lewisy antibody. The substantial expression of high mannose and complex type N-glycans terminated with Lewisx and Lewisy sequences suggests that sperm glycoproteins are highly decorated with ligands for DC-SIGN. Based on previous studies, the addition of such carbohydrate signals should inhibit antigen-specific responses directed against sperm glycoproteins in both the male and female reproductive systems. Thus, the major N-glycans of human sperm are associated with the inhibition of both innate and adaptive immune responses. These results provide more support for the eutherian fetoembryonic defense system hypothesis that links the expression of carbohydrate functional groups to the protection of gametes and the developing human in utero. This study also highlights the usefulness of glycomic profiling for revealing potential physiological functions of glycans expressed in specific cell types.
Julien S, Grimshaw MJ, Sutton-Smith M, et al., 2007, Sialyl-lewis<SUP>x</SUP> on P-selectin glycoprotein ligand-1 is regulated during differentiation and maturation of dendritic cells:: A mechanism involving the glycosyltransferases C2GnT1 and ST3Gal I, JOURNAL OF IMMUNOLOGY, Vol: 179, Pages: 5701-5710, ISSN: 0022-1767
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- Citations: 38
Goldberg D, Bern M, Parry S, et al., 2007, Automated N-glycopeptide identification using a combination of single- and tandem-MS, JOURNAL OF PROTEOME RESEARCH, Vol: 6, Pages: 3995-4005, ISSN: 1535-3893
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- Citations: 84
Parry S, Wong N-K, Easton RL, et al., 2007, The sperm agglutination antigen-1 (SAGA-1) glycoforms of CD52 are O-glycosylated, GLYCOBIOLOGY, Vol: 17, Pages: 1120-1126, ISSN: 0959-6658
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- Citations: 19
Tissot B, Gasiunas N, Powell AK, et al., 2007, Towards GAG glycomics: Analysis of highly sulfated heparins by MALDI-TOF mass spectrometry, GLYCOBIOLOGY, Vol: 17, Pages: 972-982, ISSN: 0959-6658
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- Citations: 54
Tatituri RVV, Alderwick LJ, Mishra AK, et al., 2007, Structural characterization of a partially arabinosylated lipoarabinomannan variant isolated from a <i>Corynebacterium glutamicum</i> <i>ubiA</i> mutant, MICROBIOLOGY-SGM, Vol: 153, Pages: 2621-2629, ISSN: 1350-0872
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- Citations: 14
Dell A, Chalabi S, Hitchen PG, et al., 2007, Mass Spectrometry of Glycoprotein Glycans: Glycomics and Glycoproteomics, Comprehensive glycoscience, Editors: Kamerling, Boons, Kamerling, Publisher: Elsevier Science Ltd, Pages: 69-100, ISBN: 9780444527462
This work has a very broad scope and will appeal to a wide audience as it explores theinteractions between biology, chemistry and molecular biology towards ...
Sutton-Smith M, Wong NK, Khoos K-H, et al., 2007, Analysis of protein-linked glycosylation in a sperm-somatic cell adhesion system, GLYCOBIOLOGY, Vol: 17, Pages: 553-567, ISSN: 0959-6658
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- Citations: 19
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