Imperial College London
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DrHarryWhitwell

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Lecturer in Proteomics and Integrative Data Analysis Proteom
 
 
 
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h.whitwell Website CV

 
 
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Location

 

312Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Whitwell:2016:10.1080/17435390.2016.1218080,
author = {Whitwell, H and Mackay, RM and Elgy, C and Morgan, C and Griffiths, M and Clark, H and Skipp, P and Madsen, J},
doi = {10.1080/17435390.2016.1218080},
journal = {Nanotoxicology},
pages = {1385--1394},
title = {Nanoparticles in the lung and their protein corona: the few proteins that count.},
url = {http://dx.doi.org/10.1080/17435390.2016.1218080},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The formation of protein coronae on nanoparticles (NPs) has been investigated almost exclusively in serum, despite the prevailing route of exposure being inhalation of airborne particles. In addition, an increasing number of nanomedicines, that exploit the airways as the site of delivery, are undergoing medical trials. An understanding of the effects of NPs on the airways is therefore required. To further this field, we have described the corona formed on polystyrene (PS) particles with different surface modifications and on titanium dioxide particles when incubated in human bronchoalveolar lavage fluid (BALF) from patients with pulmonary alveolar proteinosis (PAP). We show, using high-resolution quantitative mass spectrometry (MS(E)), that a large number of proteins bind with low copy numbers but that a few "core" proteins bind to all particles tested with high fidelity, averaging the surface properties of the different particles independent of the surface properties of the specific particle. The averaging effect at the particle surface means that differing cellular effects may not be due to the protein corona but due to the surface properties of the nanoparticle once inside the cell. Finally, the adherence of surfactant associated proteins (SP-A, B and D) suggests that there may be interactions with lipids and pulmonary surfactant (PSf), which could have potential in vivo health effects for people with chronic airway diseases such as asthma and chronic obstructive pulmonary disease (COPD), or those who have increased susceptibility toward other respiratory diseases.
AU  - Whitwell,H
AU  - Mackay,RM
AU  - Elgy,C
AU  - Morgan,C
AU  - Griffiths,M
AU  - Clark,H
AU  - Skipp,P
AU  - Madsen,J
DO  - 10.1080/17435390.2016.1218080
EP  - 1394
PY  - 2016///
SN  - 1743-5404
SP  - 1385
TI  - Nanoparticles in the lung and their protein corona: the few proteins that count.
T2  - Nanotoxicology
UR  - http://dx.doi.org/10.1080/17435390.2016.1218080
UR  - http://hdl.handle.net/10044/1/44800
VL  - 10
ER  -
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