Publications
110 results found
Quraishi MN, Segal J, Mullish BH, et al., 2016, National survey of practice of faecal microbiota transplantation for Clostridium difficile infection in the United Kingdom, Journal of Hospital Infection, Vol: 95, Pages: 444-445, ISSN: 0195-6701
Mullish BH, Mcdonald JA, Pechlivanis A, et al., 2016, Understanding the efficacy of faecal microbiota transplantation in Clostridium difficile infection: re-establishment of gut microbiota with the ability to degrade bile?, British Society of Gastroenterology Annual Meeting 2016, Publisher: BMJ Publishing Group, ISSN: 1468-3288
Quraishi MN, Segal J, Mullish BH, et al., 2016, National survey of practice of faecal microbiota transplantation for Clostridium difficile infection in the United Kingdom, British Society of Gastroenterology Annual Meeting 2016, Publisher: BMJ Publishing Group, ISSN: 1468-3288
Fulton AL, Williams HR, Shah J, et al., 2016, Specialist advice for primary care: an evaluation of a gastroenterology email advice service., Future Hosp J, Vol: 3, Pages: 90-93, ISSN: 2055-3323
Communication between primary and secondary care -physicians is often unreliable and one sided in the form of clinic letters. Alternatively, general practitioners (GPs) may have difficulty contacting an on-call specialist via outdated hospital paging services. At Imperial College Healthcare NHS Trust, a gastroenterology email advice line was set up to promote dialogue and potentially help GPs deal with issues within their practices. The service has been evaluated both objectively through analysis of enquiries and subjectively through a -survey of GPs' views. Analysis showed a very high level of satisfaction among users of the service. There is also good evidence to suggest that the service has helped to streamline patient management and led to the avoidance of some outpatient appointments.
Jones A, Mullish BH, Williams HRT, et al., 2016, Improvements in Clostridium difficile infection in England and comparative epidemiology with the US, 33rd International Society for Quality in Health Care Conference 2016, Publisher: Oxford University Press (OUP), ISSN: 1464-3677
Gratton J, Phetcharaburanin J, Mullish BH, et al., 2016, An optimized sample handling strategy for metabolic profiling of human feces, Analytical Chemistry, Vol: 88, Pages: 4661-4668, ISSN: 0003-2700
Fecal metabolites are being increasingly studied to unravel the host-gut microbial metabolic interactions. However, there are currently no guidelines for fecal sample collection and storage based on a systematic evaluation of the effect of time, storage temperature, storage duration and sampling strategy. Here we derive an optimized protocol for fecal sample handling with the aim of maximizing metabolic stability and minimizing sample degradation. Samples obtained from five healthy individuals were analyzed to assess topographical homogeneity of feces, and to evaluate storage duration-, temperature- and freeze-thaw cycle-induced metabolic changes in crude stool and fecal water using a 1H NMR spectroscopy-based metabolic profiling approach. Inter-individual variation was much greater than that attributable to storage conditions. Individual stool samples were found to be heterogeneous and spot sampling resulted in a high degree of metabolic variation. Crude fecal samples were remarkably unstable over time and exhibited distinct metabolic profiles at different storage temperatures. Microbial fermentation was the dominant driver in time-related changes observed in fecal samples stored at room temperature and this fermentative process was reduced when stored at 4°C. Crude fecal samples frozen at -20°C manifested elevated amino acids and nicotinate and depleted short chain fatty acids compared to crude fecal control samples. The relative concentrations of branched-chain and aromatic amino acids significantly increased in the freeze-thawed crude fecal samples, suggesting a release of microbial intracellular contents. The metabolic profiles of fecal water samples were more stable compared to crude samples. Our recommendation is that intact fecal samples should be collected, kept at 4°C or on ice during transportation, and extracted ideally within 1 h of collection, or a maximum of 24 h. Fecal water samples should be extracted from a representative amount (~15 g)
Mullish BH, Williams HR, 2015, Obstacles to establishing an NHS faecal transplant programme., BMJ, Vol: 351, Pages: h6043-h6043
Hicks LC, Huang J, Kumar S, et al., 2015, Analysis of Exhaled Breath Volatile Organic Compounds in Inflammatory Bowel Disease: A Pilot Study, JOURNAL OF CROHNS & COLITIS, Vol: 9, Pages: 731-737, ISSN: 1873-9946
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- Citations: 46
Powles STR, Hicks LC, Jimenez B, et al., 2015, Effect of co-morbidities on urinary metabolic profiling in the characterisation of patients with inflammatory bowel disease, 2nd Digestive Disorders Federation Conference, Publisher: BMJ Publishing Group, Pages: A436-A437, ISSN: 0017-5749
Hicks LC, Ralphs SJL, Williams HRT, 2015, Metabonomics and diagnostics., Methods Mol Biol, Vol: 1277, Pages: 233-244
Metabonomic techniques have considerable potential in the field of clinical diagnostics, typifying the application of a translational research paradigm. Care must be taken at all stages to apply appropriate methodology with accurate patient selection and profiling, and rigorous data acquisition and handling, to ensure clinical validity.An ever-increasing number of publications in a wide range of diseases and diverse patient groups suggest a variety of potential clinical uses; prospective studies in large validation cohorts are required to bring metabonomics into routine clinical practice. In this chapter, the utility of metabonomics as a diagnostic tool will be discussed.
Mullish BH, Marchesi JR, Thursz MR, et al., 2014, The Potential of Microbiome Manipulation as a Therapeutic Strategy in Clostridium difficile Infection., QJM, ISSN: 1460-2725
Hawtin KE, Williams HRT, McKnight L, et al., 2014, Performance in the FRCR (UK) Part 2B examination: Analysis of factors associated with success, CLINICAL RADIOLOGY, Vol: 69, Pages: 750-757, ISSN: 0009-9260
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- Citations: 1
Walker DG, Williams HRT, Bancil AS, et al., 2013, Ethnicity Differences in Genetic Susceptibility to Ulcerative Colitis: A Comparison of Indian Asians and White Northern Europeans, INFLAMMATORY BOWEL DISEASES, Vol: 19, Pages: 2888-2894, ISSN: 1078-0998
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- Citations: 10
Patel N, Blackwell VJ, Patel P, et al., 2013, THE DIAGNOSTIC UTILITY OF ENDOSCOPIC DUODENAL BIOPSIES FOR GASTROINTESTINAL INVESTIGATION, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: BMJ PUBLISHING GROUP, Pages: A285-A286, ISSN: 0017-5749
Nayagam S, Lloyd K, Byrne E, et al., 2013, Investigations for Coeliac Disease in Iron Deficiency Anaemia - Are We Following BSG Guidelines?, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S252-S252, ISSN: 0016-5085
Walker MM, Lloyd K, Byrne E, et al., 2013, Clinical Follow up of Duodenal Biopsies Showing Possible Coeliac Disease Pathology - Is Serology Performed?, Digestive Disease Week / 28th Annual Residents and Fellows Research Conference of the Society-for-Surgery-of-the-Alimentary-Tract (SSAT), Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S654-S654, ISSN: 0016-5085
Hicks L, Walker DG, Eng D, et al., 2013, Urinary Metabolic Profiling of Inflammatory Bowel Disease in a South Asian Cohort, Digestive Disease Week / 28th Annual Residents and Fellows Research Conference of the Society-for-Surgery-of-the-Alimentary-Tract (SSAT), Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S653-S653, ISSN: 0016-5085
Williams HRT, Willsmore JD, Cox IJ, et al., 2012, Serum Metabolic Profiling in Inflammatory Bowel Disease, DIGESTIVE DISEASES AND SCIENCES, Vol: 57, Pages: 2157-2165, ISSN: 0163-2116
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- Citations: 72
Nayagam S, Selvapatt N, Auguste JL, et al., 2012, QUALITY OF COLONOSCOPIC PROCEDURES AMONG INDEPENDENTLY PRACTISING GASTROENTEROLOGY TRAINEES IN A NW LONDON COHORT: ARE THEY REACHING NATIONAL STANDARDS?, GUT, Vol: 61, Pages: A59-A60, ISSN: 0017-5749
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- Citations: 1
Nayagam S, Lloyd K, Byrne E, et al., 2012, INVESTIGATIONS FOR COELIAC DISEASE IN IRON DEFICIENCY ANAEMIA-ARE WE FOLLOWING BSG GUIDELINES?, GUT, Vol: 61, Pages: A370-A371, ISSN: 0017-5749
Cobbold JFL, Cox IJ, Brown AS, et al., 2012, Lipid profiling of pre-treatment liver biopsy tissue predicts sustained virological response in patients with chronic hepatitis C, Hepatology Research, Vol: 42, Pages: 714-720, ISSN: 1386-6346
Aim: Hepatic lipid is important in the pathogenesis and progression of hepatitis C-related liver disease. Polyunsaturated fatty acids have been shown to reduce viral replication in cell culture. Proton magic angle spinning magnetic resonance spectroscopy (1H MAS MRS) enables metabolic analysis of intact tissue. The aim was to examine the relationship between hepatic lipid composition by metabolic profiling of liver tissue at baseline and treatment response to pegylated-Interferon alfa2 and Ribavirin.Methods: Baseline liver biopsy samples from 31 patients with chronic hepatitis C were analyzed histologically and by 1H MAS MRS. Indices of lipid composition were derived and partial least squares discriminant analysis with cross-validation was used to predict treatment outcome.Results: Of 31 patients, 14 achieved sustained virological response (SVR). Lipid polyunsaturation (median (IQR)) was higher in SVR (3.41% (2.31)) than in treatment failure (TF) (2.15% (1.51)), P = 0.02. Lipid saturation was lower in SVR (85.9% (3.39)) than TF (86.7% (2.17)), P = 0.04. The total lipid content was lower in SVR (1.54% (0.81)) than TF (2.72% (3.47)), P = 0.004. Total choline to lipid ratio was higher in SVR (11.51% (9.99)) than TF (7.5% (6.82)), P = 0.007. Cross-validation correctly predicted the SVR group in 13 of 14 samples with 1 sample misclassified, and the TF group in all 17 samples.Conclusions: Lipid polyunsaturation was greater and total lipid lower in those with SVR, compared with TF. Metabolic profiling of intact liver biopsy samples predicted SVR with high accuracy. Hepatic lipid composition may impact on treatment success.
Walker DG, Williams HRT, Kane SP, et al., 2011, Differences in Inflammatory Bowel Disease Phenotype between South Asians and Northern Europeans Living in North West London, UK, AMERICAN JOURNAL OF GASTROENTEROLOGY, Vol: 106, Pages: 1281-1289, ISSN: 0002-9270
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- Citations: 51
AbdAlla MSH, Taylor-Robinson SD, Sharif AW, et al., 2011, Differences in phosphatidylcholine and bile acids in bile from Egyptian and UK patients with and without cholangiocarcinoma, HPB, Vol: 13, Pages: 385-390, ISSN: 1365-182X
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- Citations: 24
Shariff MIF, Gomaa AI, Cox IJ, et al., 2011, Urinary Metabolic Biomarkers of Hepatocellular Carcinoma in an Egyptian Population: A Validation Study, JOURNAL OF PROTEOME RESEARCH, Vol: 10, Pages: 1828-1836, ISSN: 1535-3893
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- Citations: 75
Walker DG, Bancil AS, Williams HR, et al., 2011, HOW HELPFUL ARE SEROLOGICAL MARKERS IN DIFFERENTIATING CROHN'S DISEASE FROM ULCERATIVE COLITIS IN INDIAN ASIAN INFLAMMATORY BOWEL DISEASE PATIENTS?, Annual Meeting on British-Society-of-Gasenterology, Publisher: B M J PUBLISHING GROUP, ISSN: 0017-5749
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- Citations: 2
Walker DG, Bancil AS, Rai PS, et al., 2011, ETHNIC VARIATION IN THE FREQUENCY OF IBD RELATED POLYMORPHISMS IN IRGM, ATG16L1 AND IL23R, Annual Meeting on British-Society-of-Gasenterology, Publisher: B M J PUBLISHING GROUP, Pages: A223-A223, ISSN: 0017-5749
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- Citations: 5
Davies MB, Cobbold JFL, Walker DG, et al., 2011, THE PREVALENCE OF ABNORMAL HEPATIC BIOCHEMISTRY AND HEPATOBILIARY MORBIDITY IN A COHORT OF PATIENTS WITH INFLAMMATORY BOWEL DISEASE, Annual Meeting on British-Society-of-Gasenterology, Publisher: B M J PUBLISHING GROUP, ISSN: 0017-5749
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- Citations: 1
Williams HRT, Cox IJ, Walker DG, et al., 2010, Differences in gut microbial metabolism are responsible for reduced hippurate synthesis in Crohn's disease, BMC GASTROENTEROLOGY, Vol: 10, ISSN: 1471-230X
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- Citations: 72
Sharif AW, Williams HRT, Lampejo T, et al., 2010, Metabolic profiling of bile in cholangiocarcinoma using <i>in vitro</i> magnetic resonance spectroscopy, HPB, Vol: 12, Pages: 396-402, ISSN: 1365-182X
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- Citations: 37
Williams HR, Cox IJ, Walker DG, et al., 2010, Differences in Gut Microbial Metabolism are Responsible for Reduced Hippurate Synthesis in Crohn's Disease, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: S579-S579, ISSN: 0016-5085
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- Citations: 1
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