Imperial College London

DrHirokoYaguchi

Faculty of MedicineFaculty of Medicine Centre

Teaching Fellow
 
 
 
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Contact

 

+44 (0)20 7594 8652h.yaguchi

 
 
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Location

 

121Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Publication Type
Year
to

18 results found

Prawiro C, Bunney TD, Kampyli C, Yaguchi H, Katan M, Bangham CRMet al., 2023, A frequent PLCγ1 mutation in adult T-cell leukemia/lymphoma determines functional properties of the malignant cells, BBA: Molecular Basis of Disease, Vol: 1869, ISSN: 0925-4439

BackgroundDevelopment of adult T-cell leukemia/lymphoma (ATL) involves human T-cell leukemia virus type 1 (HTLV-1) infection and accumulation of somatic mutations. The most frequently mutated gene in ATL (36 % of cases) is phospholipase C gamma1 (PLCG1). PLCG1 is also frequently mutated in other T-cell lymphomas. However, the functional consequences of the PLCG1 mutations in cancer cells have not been characterized.MethodsWe compared the activity of the wild-type PLCγ1 with that of a mutant carrying a hot-spot mutation of PLCγ1 (S345F) observed in ATL, both in cells and in cell-free assays. To analyse the impact of the mutation on cellular properties, we quantified cellular proliferation, aggregation, chemotaxis and apoptosis by live cell-imaging in an S345F+ ATL-derived cell line (KK1) and a KK1 cell line in which we reverted the mutation to the wild-type sequence using CRISPR/Cas9 and homology-directed repair.FindingsThe PLCγ1 S345F mutation results in an increase of basal PLC activity in vitro and in different cell types. This higher basal activity is further enhanced by upstream signalling. Reversion of the S345F mutation in the KK1 cell line resulted in reduction of the PLC activity, lower rates of proliferation and aggregation, and a marked reduction in chemotaxis towards CCL22. The PLCγ1-pathway inhibitors ibrutinib and ritonavir reduced both the PLC activity and the tested functions of KK1 cells.InterpretationConsistent with observations from clinical studies, our data provide direct evidence that activated variants of the PLCγ1 enzyme contribute to the properties of the malignant T-cell clone in ATL.

Journal article

Bangham CRM, Melamed A, Yaguchi H, Miura M, Witkover A, Fitzgerald T, Birney Eet al., 2018, The human leukemia virus HTLV-1 alters the structure and transcription of host chromatin in cis, eLife, Vol: 7, Pages: 1-20, ISSN: 2050-084X

Chromatin looping controls gene expression by regulating promoter-enhancer contacts, the spread of epigenetic modifications, and the segregation of the genome into transcriptionally active and inactive compartments. We studied the impact on the structure and expression of host chromatin by the human retrovirus HTLV-1. We show that HTLV-1 disrupts host chromatin structure by forming loops between the provirus and the host genome; certain loops depend on the critical chromatin architectural protein CTCF, which we recently discovered binds to the HTLV-1 provirus. We show that the provirus causes two distinct patterns of abnormal transcription of the host genome in cis: bidirectional transcription in the host genome immediately flanking the provirus, and clone-specific transcription in cis at non-contiguous loci up to >300 kb from the integration site. We conclude that HTLV-1 causes insertional mutagenesis up to the megabase range in the host genome in >104 persistently-maintained HTLV-1+ T-cell clones in vivo.

Journal article

Cook L, Melamed A, Yaguchi H, Bangham CRet al., 2017, The impact of HTLV-1 on the cellular genome., Current Opinion in Virology, Vol: 26, Pages: 125-131, ISSN: 1879-6265

Human T-lymphotropic virus type-1 (HTLV-1) is the causative agent of adult T-cell leukaemia/lymphoma (ATL), an aggressive CD4+ T-cell malignancy. The mechanisms of leukaemogenesis in ATL are incompletely understood. Insertional mutagenesis has not previously been thought to contribute to the pathogenesis of ATL. However, the recent discovery that HTLV-1 binds the key chromatin architectural protein CTCF raises the hypothesis that HTLV-1 deregulates host gene expression by causing abnormal chromatin looping, bringing the strong HTLV-1 promoter-enhancer near to host genes that lie up to 2Mb from the integrated provirus. Here we review current opinion on the mechanisms of oncogenesis in ATL, with particular emphasis on the local and distant impact of HTLV-1 on the structure and expression of the host genome.

Journal article

Satou Y, Miyazato P, Ishihara Y, Yaguchi H, Melamed A, Miura M, Fukuda A, Nosaka K, Watanabe T, Rowan A, Nakao M, Bangham Cet al., 2016, The retrovirus HTLV-1 inserts an ectopic CTCF-binding site into the human genome, Proceedings of the National Academy of Sciences of the United States of America, Vol: 113, Pages: 3054-3059, ISSN: 0027-8424

Human T-lymphotropic virus type 1 (HTLV-1) is a retrovirus thatcauses malignant and inflammatory diseases in 10% of infectedpeople. A typical host has between 104and 105clones of HTLV-1-infected T lymphocytes, each clone distinguished by the genomicintegration site of the single-copy HTLV-1 provirus. TheHBZgeneis constitutively expressed from the minus strand of the provirus,whereas plus-strand expression, required for viral propagation touninfected cells, is suppressed or intermittentin vivo, allowingescape from host immune surveillance. It remains unknown whatregulates this pattern of proviral transcription and latency. Herewe show that CTCF, a key regulator of chromatin structure andfunction, binds to the provirus at a sharp border in epigeneticmodifications in the pX region of the HTLV-1 provirus, in T cellsnaturally infected with HTLV-1. CTCF is a zinc-finger protein thatbinds to an insulator region in genomic DNA and plays a funda-mental role in controlling higher-order chromatin structure andgene expression in vertebrate cells. We show that CTCF boundto HTLV-1 acts as an enhancer blocker, regulates HTLV-1 mRNAsplicing, and forms long-distance interactions with flanking hostchromatin. CTCF binding sites have been propagated through-out the genome by transposons in certain primate lineages, butCTCF binding has not previously been described in present-dayexogenous retroviruses. The presence of an ectopic CTCF bindingsite introduced by the retrovirus in tens of thousands of genomiclocations has the potential to cause widespread abnormalities inhost cell chromatin structure and gene expression.

Journal article

Melamed A, Yaguchi H, Rowan A, Cook L, Bangham CRMet al., 2015, Comparative analysis of gene expression patterns in the HTLV-1 infected T-cell clones, Publisher: BIOMED CENTRAL LTD, ISSN: 1742-4690

Conference paper

Yaguchi H, Melamed A, Rowan A, Cook L, Satou Y, Bangham CRMet al., 2015, Identification of long-range chromatin interactions between HTLV-1 and the host genome, Publisher: BIOMED CENTRAL LTD, ISSN: 1742-4690

Conference paper

Eguren M, Alvarez-Fernandez M, Garcia F, Lopez-Contreras AJ, Fujimitsu K, Yaguchi H, Luis Luque-Garcia J, Fernandez-Capetillo O, Munoz J, Yamano H, Malumbres Met al., 2014, A Synthetic Lethal Interaction between APC/C and Topoisomerase Poisons Uncovered by Proteomic Screens, CELL REPORTS, Vol: 6, Pages: 670-683, ISSN: 2211-1247

Journal article

Shimazu S, Nagamura Y, Yaguchi H, Ohkura N, Tsukada Tet al., 2011, Correlation of mutant menin stability with clinical expression of multiple endocrine neoplasia type 1 and its incomplete forms, CANCER SCIENCE, Vol: 102, Pages: 2097-2102, ISSN: 1347-9032

Journal article

Kitoh A, Ono M, Naoe Y, Ohkura N, Yamaguchi T, Yaguchi H, Kitabayashi I, Tsukada T, Nomura T, Miyachi Y, Taniuchi I, Sakaguchi Set al., 2009, Indispensable Role of the Runx1-Cbfβ Transcription Complex for In Vivo-Suppressive Function of FoxP3<SUP>+</SUP> Regulatory T Cells, IMMUNITY, Vol: 31, Pages: 609-620, ISSN: 1074-7613

Journal article

Ono M, Yaguchi H, Ohkura N, Kitabayashi I, Nagamura Y, Nomura T, Miyachi Y, Tsukada T, Sakaguchi Set al., 2007, Foxp3 controls regulatory T-cell function by interacting with AML1/Runx1, NATURE, Vol: 446, Pages: 685-689, ISSN: 0028-0836

Journal article

Fukuuchi A, Nagamura Y, Yaguchi H, Ohkura N, Obara T, Tsukada Tet al., 2006, A whole <i>MEN1</i> gene deletion flanked by Alu repeats in a family with multiple endocrine neoplasia type 1, JAPANESE JOURNAL OF CLINICAL ONCOLOGY, Vol: 36, Pages: 739-744, ISSN: 0368-2811

Journal article

Takahashi M, Kikuchi M, Ohkura N, Yaguchi H, Nagamura Y, Ohnami S, Ushiama M, Yoshida T, Sugano K, Iwama T, Kosugi S, Tsukada Tet al., 2006, Detection of <i>APC</i> gene deletion by double competitive polymerase chain reaction in patients with familial adenomatous polyposis, INTERNATIONAL JOURNAL OF ONCOLOGY, Vol: 29, Pages: 413-421, ISSN: 1019-6439

Journal article

Ohkura N, Takahashi M, Yaguchi H, Nagamura Y, Tsukada Tet al., 2005, Coactivator-associated arginine methyltransferase 1, CARM1, affects pre-mRNA splicing in an isoform-specific manner, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 280, Pages: 28927-28935

Journal article

Yaguchi H, Ohkura N, Takahashi M, Nagamura Y, Kitabayashi I, Tsukada Tet al., 2004, Menin missense mutants associated with multiple endocrine neoplasia type 1 are rapidly degraded via the ubiquitin-proteasome pathway, MOLECULAR AND CELLULAR BIOLOGY, Vol: 24, Pages: 6569-6580, ISSN: 0270-7306

Journal article

Miyakoshi J, Mori Y, Yaguchi H, Ding GR, Fujimori Aet al., 2000, Suppression of heat-induced hsp-70 by simultaneous exposure to 50 mT magnetic field, LIFE SCIENCES, Vol: 66, Pages: 1187-1196, ISSN: 0024-3205

Journal article

MURAO M, TERAI T, ABE S, OSAKI Y, YAGUCHI H, OGI M, KON H, MIKAMI T, SUZUKI K, SASAKI Y, MORIYAMA Y, KURODA R, NASUHARA K, SHIMOMURA J, HARADA K, KONDO Ket al., 1980, CLINICAL-EVALUATION OF NEW CEPHALOSPORIN, CEFTIZOXIME, FOR RESPIRATORY-INFECTION, CHEMOTHERAPY-TOKYO, Vol: 28, Pages: 148-140, ISSN: 0009-3165

Journal article

KURIMURA O, TAMAKI K, SASAKI H, MURAI T, NOZAKI K, MATSUKI S, DOI H, YAGUCHI H, YOKOTA K, SHIMONAKA A, MORIOKA Yet al., 1980, LABORATORY AND CLINICAL-EVALUATION OF CEFOTAXIME, CHEMOTHERAPY-TOKYO, Vol: 28, Pages: 379-386, ISSN: 0009-3165

Journal article

KURIMURA O, TAMAKI K, SASAKI H, MORIOKA Y, DOI H, YAGUCHI H, SUEDA Ket al., 1980, BASIC AND CLINICAL-STUDIES ON CEFADROXIL, CHEMOTHERAPY-TOKYO, Vol: 28, Pages: 234-238, ISSN: 0009-3165

Journal article

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