Imperial College London
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Dr Hannah M Cheeseman

Faculty of Medicine, Department of Infectious Disease

Postdoctoral Researcher & Clinical Trials Laboratory Manager
 
 
 
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Contact

 

+44 (0)20 7594 2540hannah.cheeseman

 
 
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Location

 

452 - Shattock GroupNorfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cole:2022:10.1007/978-1-0716-1900-1_23,
author = {Cole, ME and Guo, Y and Cheeseman, HM and Pollock, KM},
doi = {10.1007/978-1-0716-1900-1_23},
journal = {Methods Mol Biol},
pages = {433--447},
title = {Multicolor Flow Cytometry and High-Dimensional Data Analysis to Probe Complex Questions in Vaccinology.},
url = {http://dx.doi.org/10.1007/978-1-0716-1900-1_23},
volume = {2414},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Vaccines induce a highly complex immune reaction in secondary lymphoid organs to generate immunological memory against an antigen or antigens of interest. Measurement of post immunization immune responses generated by specialized lymphocyte subsets requires time-dependent sampling, usually of the blood. Several T and B cell subsets are involved in the reaction, including CD4 and CD8 T cells, T follicular helper cells (Tfh), and germinal center B cells alongside their circulating (c) counterparts; cTfh and antibody secreting cells. Multicolor flow cytometry of peripheral blood mononuclear cells (PBMC) coupled with high-dimensional analysis offers an opportunity to study these cells in detail. Here we demonstrate a method by which such data can be generated and analysed using software that renders multidimensional data on a two dimensional map to identify rare vaccine-induced T and B cell subsets.
AU  - Cole,ME
AU  - Guo,Y
AU  - Cheeseman,HM
AU  - Pollock,KM
DO  - 10.1007/978-1-0716-1900-1_23
EP  - 447
PY  - 2022///
SP  - 433
TI  - Multicolor Flow Cytometry and High-Dimensional Data Analysis to Probe Complex Questions in Vaccinology.
T2  - Methods Mol Biol
UR  - http://dx.doi.org/10.1007/978-1-0716-1900-1_23
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34784050
VL  - 2414
ER  -
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