Imperial College London
Alternate

DrHelenaCocheme

Faculty of Medicine, Institute of Clinical Sciences

Honorary Senior Lecturer
 
 
 
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Contact

 

helena.cocheme Website

 
 
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Location

 

4.15ELMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Logan:2015:10.1016/j.cmet.2015.11.014,
author = {Logan, A and Pell, VR and Shaffer, KJ and Evans, C and Stanley, NJ and Robb, EL and Prime, TA and Chouchani, ET and Cochemé, HM and Fearnley, IM and Vidoni, S and James, AM and Porteous, CM and Partridge, L and Krieg, T and Smith, RA and Murphy, MP},
doi = {10.1016/j.cmet.2015.11.014},
journal = {Cell Metabolism},
pages = {379--385},
title = {Assessing the mitochondrial membrane potential in cells and in vivo using targeted click chemistry and mass spectrometry},
url = {http://dx.doi.org/10.1016/j.cmet.2015.11.014},
volume = {23},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The mitochondrial membrane potential (Δψm) is a major determinant and indicator of cell fate, but it is not possible to assess small changes in Δψm within cells or in vivo. To overcome this, we developed an approach that utilizes two mitochondria-targeted probes each containing a triphenylphosphonium (TPP) lipophilic cation that drives their accumulation in response to Δψm and the plasma membrane potential (Δψp). One probe contains an azido moiety and the other a cyclooctyne, which react together in a concentration-dependent manner by "click" chemistry to form MitoClick. As the mitochondrial accumulation of both probes depends exponentially on Δψm and Δψp, the rate of MitoClick formation is exquisitely sensitive to small changes in these potentials. MitoClick accumulation can then be quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This approach enables assessment of subtle changes in membrane potentials within cells and in the mouse heart in vivo.
AU  - Logan,A
AU  - Pell,VR
AU  - Shaffer,KJ
AU  - Evans,C
AU  - Stanley,NJ
AU  - Robb,EL
AU  - Prime,TA
AU  - Chouchani,ET
AU  - Cochemé,HM
AU  - Fearnley,IM
AU  - Vidoni,S
AU  - James,AM
AU  - Porteous,CM
AU  - Partridge,L
AU  - Krieg,T
AU  - Smith,RA
AU  - Murphy,MP
DO  - 10.1016/j.cmet.2015.11.014
EP  - 385
PY  - 2015///
SN  - 1932-7420
SP  - 379
TI  - Assessing the mitochondrial membrane potential in cells and in vivo using targeted click chemistry and mass spectrometry
T2  - Cell Metabolism
UR  - http://dx.doi.org/10.1016/j.cmet.2015.11.014
UR  - http://hdl.handle.net/10044/1/31385
VL  - 23
ER  -
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