Imperial College London
Alternate

DrHelenaCocheme

Faculty of Medicine, Institute of Clinical Sciences

Honorary Senior Lecturer
 
 
 
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Contact

 

helena.cocheme Website

 
 
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Location

 

4.15ELMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Logan:2013:10.1016/j.bbagen.2013.05.026,
author = {Logan, A and Cochemé, HM and Li, Pun PB and Apostolova, N and Smith, RA and Larsen, L and Larsen, DS and James, AM and Fearnley, IM and Rogatti, S and Prime, TA and Finichiu, PG and Dare, A and Chouchani, ET and Pell, VR and Methner, C and Quin, C and McQuaker, SJ and Krieg, T and Hartley, RC and Murphy, MP},
doi = {10.1016/j.bbagen.2013.05.026},
journal = {Biochimica et Biophysica Acta - General Subjects},
pages = {923--930},
title = {Using exomarkers to assess mitochondrial reactive species in vivo},
url = {http://dx.doi.org/10.1016/j.bbagen.2013.05.026},
volume = {1840},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: The ability to measure the concentrations of small damaging and signalling molecules such as reactive oxygen species (ROS) in vivo is essential to understanding their biological roles. While a range of methods can be applied to in vitro systems, measuring the levels and relative changes in reactive species in vivo is challenging. SCOPE OF REVIEW: One approach towards achieving this goal is the use of exomarkers. In this, exogenous probe compounds are administered to the intact organism and are then transformed by the reactive molecules in vivo to produce a diagnostic exomarker. The exomarker and the precursor probe can be analysed ex vivo to infer the identity and amounts of the reactive species present in vivo. This is akin to the measurement of biomarkers produced by the interaction of reactive species with endogenous biomolecules. MAJOR CONCLUSIONS AND GENERAL SIGNIFICANCE: Our laboratories have developed mitochondria-targeted probes that generate exomarkers that can be analysed ex vivo by mass spectrometry to assess levels of reactive species within mitochondria in vivo. We have used one of these compounds, MitoB, to infer the levels of mitochondrial hydrogen peroxide within flies and mice. Here we describe the development of MitoB and expand on this example to discuss how better probes and exomarkers can be developed. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.
AU  - Logan,A
AU  - Cochemé,HM
AU  - Li,Pun PB
AU  - Apostolova,N
AU  - Smith,RA
AU  - Larsen,L
AU  - Larsen,DS
AU  - James,AM
AU  - Fearnley,IM
AU  - Rogatti,S
AU  - Prime,TA
AU  - Finichiu,PG
AU  - Dare,A
AU  - Chouchani,ET
AU  - Pell,VR
AU  - Methner,C
AU  - Quin,C
AU  - McQuaker,SJ
AU  - Krieg,T
AU  - Hartley,RC
AU  - Murphy,MP
DO  - 10.1016/j.bbagen.2013.05.026
EP  - 930
PY  - 2013///
SN  - 0006-3002
SP  - 923
TI  - Using exomarkers to assess mitochondrial reactive species in vivo
T2  - Biochimica et Biophysica Acta - General Subjects
UR  - http://dx.doi.org/10.1016/j.bbagen.2013.05.026
UR  - http://hdl.handle.net/10044/1/40460
VL  - 1840
ER  -
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