Publications
137 results found
THOMAS HJW, 1993, THE TIMING OF P53 INACTIVATION IN CHRONIC ULCERATIVE-COLITIS, GASTROENTEROLOGY, Vol: 104, Pages: 1889-1890, ISSN: 0016-5085
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- Citations: 1
THOMAS HJW, FISH DE, PRICE AB, 1993, IMMUNOHISTOCHEMICAL DETECTION OF P53 IN EPITHELIAL DYSPLASIA ASSOCIATED WITH ULCERATIVE-COLITIS, GASTROENTEROLOGY, Vol: 104, Pages: A455-A455, ISSN: 0016-5085
HAMPTON GM, WARD J, COTTRELL S, et al., 1992, YEAST ARTIFICIAL CHROMOSOMES FOR THE MOLECULAR ANALYSIS OF THE FAMILIAL POLYPOSIS APC GENE REGION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 89, Pages: 8249-8253, ISSN: 0027-8424
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- Citations: 11
Hampton GM, Ward JR, Cottrell S, et al., 1992, Yeast artificial chromosomes for the molecular analysis of the familial polyposis APC gene region., Proc Natl Acad Sci U S A, Vol: 89, Pages: 8249-8253, ISSN: 0027-8424
Two yeast artificial chromosomes (YACs) spanning a total distance of 1.1 megabase pairs of DNA around the MCC (for mutated in colorectal carcinoma) and APC (for adenomatous polyposis coli) genes at 5q21 have been isolated and characterized. Starting from the MCC gene, a strategy was undertaken to identify constitutional submicroscopic deletions in familial adenomatous polyposis patients that might considerably narrow down the position of the APC gene. To this end, YACs identified by the MCC gene were screened across a chromosome 5-specific cosmid library to provide a source of DNA probes for genomic scanning. The cosmids isolated from these experiments were used to screen a panel of somatic cell hybrids containing chromosome 5 segregated from patients suspected to carry putative interstitial deletions. This screening approach led to the confirmation of a small heterozygous deletion in a polyposis patient that overlaps one of the two isolated YACs. This YAC has been shown to contain the entire APC gene, in addition to a significant portion of DNA flanking the 5' end of the gene, and should therefore prove a valuable resource for functional studies by transfer to colorectal tumor-derived cell lines.
Thomas HJ, 1991, The genetics and molecular biology of colorectal cancer., Curr Opin Oncol, Vol: 3, Pages: 702-710, ISSN: 1040-8746
Progress has continued in explicating the genetics of inherited predispositions to colorectal cancer and the molecular events that lead to tumorigenesis. New polymorphic DNA markers have been described for the presymptomatic diagnosis of familial adenomatous polyposis and a candidate gene has been characterized. Pedigree studies have suggested that there may be an inherited predisposition to many apparently nonfamilial colorectal cancers and a genetic model of tumorigenesis in common colorectal cancer has been proposed that includes the activation of dominantly acting oncogenes and the inactivation of growth suppressor genes. Two features of colorectal cancer make it a particularly good model for studying neoplasia. There are several well-defined rare inherited syndromes that predispose to the development of colorectal cancer in an autosomal dominant manner, and the majority of carcinomas are thought to arise from preexisting benign adenomatous polyps, allowing the analysis of genetic changes during tumorigenesis.
WIRTH BI, RUTHER K, THOMAS HJ, et al., 1991, MOLECULAR GENETIC-STUDY OF THE REGION AROUND THE SPINAL MUSCULAR-ATROPHY GENE - ENRICHING THE REGION WITH FURTHER MARKERS, CYTOGENETICS AND CELL GENETICS, Vol: 58, Pages: 1906-1906, ISSN: 0301-0171
WARD JR, THOMAS HJW, COTTRELL S, et al., 1991, PHYSICAL MAPPING OF 3 DELETIONS INCLUDING THE APC GENE, CYTOGENETICS AND CELL GENETICS, Vol: 58, Pages: 1905-1905, ISSN: 0301-0171
Jeremiah SJ, Abbott CM, Murad Z, et al., 1990, The assignment of the genes coding for human complement components C6 and C7 to chromosome 5., Ann Hum Genet, Vol: 54, Pages: 141-147, ISSN: 0003-4800
A panel of 19 somatic cell hybrids was tested for the presence of human sequences coding for complement components C6 and C7 by restriction enzyme digestion and Southern blots probed with human C6 and C7 cDNA probes. C7 was also detected by amplifying part of the human gene in hybrid DNA using the polymerase chain reaction. Detection of human C6 and C7 was completely correlated with the presence of chromosome 5.
JEREMIAH SJ, ABBOTT CM, MURAD Z, et al., 1990, THE ASSIGNMENT OF THE GENES-CODING FOR HUMAN-COMPLEMENT COMPONENT-C6 AND COMPONENT-C7 TO CHROMOSOME-5, ANNALS OF HUMAN GENETICS, Vol: 54, Pages: 141-147, ISSN: 0003-4800
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- Citations: 30
BISHOP DT, THOMAS HJW, 1990, THE GENETICS OF COLORECTAL-CANCER, CANCER SURVEYS, Vol: 9, Pages: 585-604, ISSN: 0261-2429
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- Citations: 46
Bishop DT, Thomas HJ, 1990, The genetics of colorectal cancer., Cancer Surv, Vol: 9, Pages: 585-604, ISSN: 0261-2429
Two features of colorectal cancer have greatly aided the recent progress in understanding its genetics: firstly the majority of colorectal cancers arise from premalignant adenomatous polyps allowing the analysis of somatic genetic changes during tumorigenesis, and secondly there are several well defined inherited syndromes that predispose to colorectal cancer in an autosomal dominant manner. The familial polyposis gene has been mapped to chromosome 5q and loss of material on chromosome 5 shown in a large proportion of sporadic (non-familial) adenomas and carcinomas. Allele loss has also been found in a high proportion of colorectal cancers on chromosomes 17 and 18 and the respective genes involved identified as that coding for the oncoprotein p53 on 17p and the DCC ('deleted in colorectal carcinomas') gene on 18q. In addition activation of k-ras is found frequently in colorectal adenomas and carcinomas. The development of colorectal neoplasia is associated with the accumulation of genetic changes. Family studies of apparently sporadic colorectal cancer probands have shown an increased incidence of adenomas and carcinomas in first degree relatives. More recently pedigree studies have suggested that an inherited predisposition may be responsible for the majority of colorectal tumours.
VARESCO L, THOMAS HJW, COTTRELL S, et al., 1989, CPG ISLAND CLONES FROM A DELETION ENCOMPASSING THE GENE FOR ADENOMATOUS POLYPOSIS COLI, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 86, Pages: 10118-10122, ISSN: 0027-8424
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- Citations: 45
VARESCO L, THOMAS HJ, WILLIAMS S, et al., 1989, CLONES FROM A DELETION ENCOMPASSING THE ADENOMATOUS POLYPOSIS COLI GENE (APC), CYTOGENETICS AND CELL GENETICS, Vol: 51, Pages: 1098-1098, ISSN: 0301-0171
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- Citations: 9
MURDAY V, COTTRELL S, BODMER WF, et al., 1989, FINE LINKAGE MAP AROUND THE ADENOMATOUS POLYPOSIS (APC) GENE, CYTOGENETICS AND CELL GENETICS, Vol: 51, Pages: 1049-1049, ISSN: 0301-0171
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- Citations: 4
JEREMIAH SJ, WEST LF, ABBOTT CM, et al., 1989, 3 GENES-CODING FOR LATE ACTING COMPONENTS OF COMPLEMENT ASSIGNED TO CHROMOSOME-5, CYTOGENETICS AND CELL GENETICS, Vol: 51, Pages: 1019-1019, ISSN: 0301-0171
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- Citations: 7
Mason AC, Thomas HJ, Barras C, et al., 1987, A study of the use of intravenous cannulas for medical emergencies in Newham--implications for financial savings., Postgrad Med J, Vol: 63, Pages: 467-469, ISSN: 0032-5473
A study of intravenous (i.v.) cannula usage for medical emergencies admitted to hospitals in the Newham Health District was undertaken during two defined periods (24 and 35 days). Almost half the cannulas inserted (47%) were not flushed following an initial bolus injection of heparinized saline. The duration that cannulas remained in a vein ranged from 24 hours to 8 days (median 2 days) and inflammation around the cannula site was related to the length of time since insertion but unrelated to whether the cannula was flushed regularly or to the type of fluid used. Our findings indicate a substantial wastage of i.v. cannulas due to difficulties with insertion and suggest that isotonic saline, without heparin, is effective in maintaining cannula patency for 48 hours. It is concluded that these findings are not unique to the Newham Health District and worthwhile financial savings should be achieved throughout the NHS if clinicians reconsider the indications and use of i.v. cannulas for their patients.
MASON AC, THOMAS HJW, BARRAS C, et al., 1987, A STUDY OF THE USE OF INTRAVENOUS CANNULAS FOR MEDICAL EMERGENCIES IN NEWHAM - IMPLICATIONS FOR FINANCIAL SAVINGS, POSTGRADUATE MEDICAL JOURNAL, Vol: 63, Pages: 467-469, ISSN: 0032-5473
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- Citations: 3
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