Imperial College London
Alternate

DrIainDunlop

Faculty of Engineering, Department of Materials

Reader in Biomaterials and Cell Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6731i.dunlop

 
 
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Location

 

1.02Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kwong:2019:10.1038/s41598-019-50412-9,
author = {Kwong, Hong Tsang D and Lieberthal, T and Watts, C and Del, Rio Hernandez A and Dunlop, I and Ramadan, S and Klein, N},
doi = {10.1038/s41598-019-50412-9},
journal = {Scientific Reports},
title = {Chemically functionalised graphene FET biosensor for the label-free sensing of exosomes},
url = {http://dx.doi.org/10.1038/s41598-019-50412-9},
volume = {9},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A   graphene   field-effect   transistor   (gFET)   was non-covalently   functionalised   with   1-pyrenebutyric  acid  N-hydroxysuccinimide  ester  and  conjugated  with  anti-CD63  antibodiesfor  the  label-free  detection of  exosomes.Using  a  microfluidic  channel,  part  of  a  graphene film  was  exposed  to solution.  The  change  in  electrical  properties  of  the  exposed  graphene created anadditional minimum alongside the original Dirac point inthe drain-source current(Ids)-back-gate voltage (Vg) curve. When phosphate buffered saline (PBS) was present in the  channel,  the  additional  minimum  was  present  at  a Vglower  than  the  original  Dirac point  and  shifted  with  time  when  exosomes  were  introduced into  the  channel.Thisshift of  the  minimum  from  the  PBS  reference  point  reached saturationafter  30  minutesand was  observed  for  multiple  exosome  concentrations. Upon  conjugation  with  an  isotype control, sensor  responsetothe  highest  concentration  ofexosomes  was  negligible  in comparison to that with anti-CD63antibody, indicatingthat thefunctionalised gFETcan specifically detect exosomes at least down to 0.1μg/mLand is sensitive to concentration. Such  a  gFET  biosensor  has  not  been  used before for  exosome  sensing  and  could  be  an effective  tool  for  the  liquid-biopsy  detection  of  exosomes  as  biomarkers  for  early-stage identification of diseases such as cancer.
AU  - Kwong,Hong Tsang D
AU  - Lieberthal,T
AU  - Watts,C
AU  - Del,Rio Hernandez A
AU  - Dunlop,I
AU  - Ramadan,S
AU  - Klein,N
DO  - 10.1038/s41598-019-50412-9
PY  - 2019///
SN  - 2045-2322
TI  - Chemically functionalised graphene FET biosensor for the label-free sensing of exosomes
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-019-50412-9
UR  - http://hdl.handle.net/10044/1/73270
VL  - 9
ER  -
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