Imperial College London
Alternate

DrIainDunlop

Faculty of Engineering, Department of Materials

Reader in Biomaterials and Cell Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6731i.dunlop

 
 
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Location

 

1.02Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Güney:2021:10.1016/j.isci.2021.103061,
author = {Güney, TG and Herranz, AM and Mumby, S and Dunlop, IE and Adcock, IM},
doi = {10.1016/j.isci.2021.103061},
journal = {iScience},
pages = {1--16},
title = {Epithelial-stromal cell interactions and ECM mechanics drive the formation of airway-mimetic tubular morphology in lung organoids},
url = {http://dx.doi.org/10.1016/j.isci.2021.103061},
volume = {24},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Complex human airway cellular organisation where extracellular matrix (ECM), epithelial and stromal lineages interact present challenges for organ study in vitro. Current in vitro lung models, that focus on the lung epithelium do not represent complex airway morphology and cell-ECM interactions seen in vivo.Models including stromal populations often separate them via a semipermeable barrier precluding cell-cell interaction or the effect of ECM mechanics. We investigated the effect of stromal cells on basal epithelial cell-derived bronchosphere structure and function through a triple culture of human bronchial epithelial, lung fibroblast and airway smooth muscle cells. Epithelial-stromal cross-talk resulted in epithelial cell-driven branching tubules with stromal cells surrounding epithelial cells termed bronchotubules. Agarose-matrigel scaffold (Agrigel) formed a mechanically tuneable ECM, with adjustable viscoelasticity and stiffness enabling long-term tubule survival. Bronchotubule models may enable research into how epithelial-stromal cell and cell-ECM communication drive tissue patterning, repair and development of disease.
AU  - Güney,TG
AU  - Herranz,AM
AU  - Mumby,S
AU  - Dunlop,IE
AU  - Adcock,IM
DO  - 10.1016/j.isci.2021.103061
EP  - 16
PY  - 2021///
SN  - 2589-0042
SP  - 1
TI  - Epithelial-stromal cell interactions and ECM mechanics drive the formation of airway-mimetic tubular morphology in lung organoids
T2  - iScience
UR  - http://dx.doi.org/10.1016/j.isci.2021.103061
UR  - https://www.sciencedirect.com/science/article/pii/S2589004221010294?via%3Dihub
UR  - http://hdl.handle.net/10044/1/91469
VL  - 24
ER  -
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