Imperial College London
Alternate

DrIainDunlop

Faculty of Engineering, Department of Materials

Reader in Biomaterials and Cell Engineering
 
 
 
//

Contact

 

+44 (0)20 7594 6731i.dunlop

 
 
//

Location

 

1.02Royal School of MinesSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Shahal:2012:10.1007/s13758-012-0023-0,
author = {Shahal, T and Geiger, B and Dunlop, IE and Spatz, JP},
doi = {10.1007/s13758-012-0023-0},
journal = {Biointerphases: an open access journal for the biomaterials interface community},
title = {Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor},
url = {http://dx.doi.org/10.1007/s13758-012-0023-0},
year = {2012}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Substrates coated with specific bioactiveligands are important for tissue engineering, enabling the local presentation of extracellular stimulants at controlled positions and densities. In this study, we examined the cross-talk between integrin and epidermal growth factor (EGF) receptors following their interaction with surfaceimmobilized Arg-Gly-Asp (RGD) and EGF ligands, respectively. Surfaces of glass coverslips, modified with biotinylated silane-polyethylene glycol, were functionalized by either biotinylated RGD or EGF (or both) via the biotin–NeutrAvidin interaction. Fluorescent labeling of the adhering A431 epidermoid carcinoma cells for zyxin or actin indicated that EGF had a dual effect on focal adhesions (FA) and stress fibers: at low concentrations (0.1; 1 ng/ml), it stimulated their growth; whereas at higher concentrations, on surfaces with low to intermediate RGD densities, it induced their disassembly, leading to cell detachment. The EGF- dependent dissociation of FAs was, however, attenuated on higher RGD density surfaces. Simultaneous stimulation by both immobilized RGD and EGF suggest a strong synergy between integrin and EGFR signaling, in FA induction and cell spreading. A critical threshold level of EGF was required to induce significant variation in cell adhesion; beyond this critical density, the immobilized molecule had a considerably stronger effect on cell adhesion than did soluble EGF. The mechanismsunderlying this synergy
AU  - Shahal,T
AU  - Geiger,B
AU  - Dunlop,IE
AU  - Spatz,JP
DO  - 10.1007/s13758-012-0023-0
PY  - 2012///
TI  - Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor
T2  - Biointerphases: an open access journal for the biomaterials interface community
UR  - http://dx.doi.org/10.1007/s13758-012-0023-0
ER  -
Download